中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (21): 3887-3890.doi: 10.3969/j.issn.1673-8225.2011.21.020

• 膜生物材料 membrane biomaterials • 上一篇    下一篇

壳聚糖/聚己内酯共混膜的制备及性能

闵  翔1，2，唐敏健1，2，焦延鹏1，2，周长忍1，2   

  1. 暨南大学，1材料科学与工程系，2人工器官及材料教育部工程研究中心，广东省广州市  510632
  • 收稿日期:2011-01-26 修回日期:2011-04-25 出版日期:2011-05-21 发布日期:2011-05-21
  • 通讯作者: 周长忍，教授，博士生导师，暨南大学材料科学与工程系，人工器官及材料教育部工程研究中心，广东省广州市 510630 tcrz9@jnu.edu.cn
  • 作者简介:闵翔★，男，1986年生，安徽省合肥市人，汉族，暨南大学理工学院在读硕士，主要从事生物材料的研究。 minxiang@yeah.net
  • 基金资助:

    国家自然科学基金(50903039)；高等教育博士计划研究基金(20094401120006)；广东省自然科学基金(9451063201002459)；重点院校基础研究基金(21609205)。

Preparation and properties of chitosan/poly(ε-caprolactone) blend films

Min Xiang1,2, Tang Min-jian1,2, Jiao Yan-peng1,2 , Zhou Chang-ren1,2   

  1. 1Department of Materials Science and Engineering, Jinan University, Guangzhou  510632, Guangdong Province, China; 2Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou  510632, Guangdong Province, China
  • Received:2011-01-26 Revised:2011-04-25 Online:2011-05-21 Published:2011-05-21
  • Contact: Zhou Chang-ren, Professor, Doctoral supervisor, Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, Guangdong Province, China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, Guangdong Province, China tcrz9@jnu.edu.cn
  • About author:Min Xiang★, Studying for master’s degree, Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, Guangdong Province, China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, Guangdong Province, China minxiang@yeah.net
  • Supported by:

    the National Natural Science Foundation of China, No. 50903039*; Doctoral Program of Higher Education Research Funding, No. 20094401120006*; the Natural Science Foundation of Guangdong Province, No. 9451063201002459*; Basic Research Funding for Key Colleges, No. 21609205*

PDF

409

被引次数

15

摘要：

背景：壳聚糖/聚己内酯共混材料在生物材料领域具有广泛的应用前景，但与蛋白、细胞反应机制尚不明确。
目的：观察壳聚糖/聚己内酯共混膜表面蛋白黏附和细胞活性。
方法：将不同配比的壳聚糖/聚己内酯混合溶液旋转涂膜法成膜。分别通过原子力显微镜、滴形分析仪、石英晶体天平和MTT比色法测量膜的表面形貌、亲疏水性、蛋白吸附和细胞增殖活性。
结果与结论：膜的表面形貌、亲疏水性、蛋白吸附和细胞增殖活性在很大程度上取决于壳聚糖和聚己内酯的质量配比。细胞在壳聚糖膜上具有较好的伸展形态，在聚己内酯膜上具有较高的增殖活性。

关键词: 壳聚糖, 聚己内酯, 共混膜, 蛋白质黏附, 牛血清白蛋白, 细胞活性

Abstract:

BACKGROUND: Chitosan/poly(ε-caprolactone) (Chitosan/PCL) blend has a perspective in biomaterials. However, it is still unknown that the correlation between protein adsorption and cell activity on blends.
OBJECTIVE: To investigation the correlation between protein adsorption behaviors and cell activities on chitosan/PCL blend films
METHODS: Chitosan/PCL films with different mass ratios were prepared by spin coating with chitosan/PCL mixture solution. Atomic force microscopy (AFM), drop shape analyzer (DSA), quartz crystal microbalance (QCM) and MTT method were used to analyze the surface morphology, hydrophilicity, protein adsorption and cell metabolic activity of films.
RESULTS AND CONCLUSION: The topography, hydrophilicity, protein adsorption and cell metabolic activity of films are largely related to the mass ratio of chitosan and PCL. The cell metabolic activity observed on hydrophobic PCL films is superior and cell adhesion level on hydrophilic chitosan films is excellent.

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