中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (21): 3851-3854.doi: 10.3969/j.issn.1673-8225.2011.21.012

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

改良自乳化溶剂扩散法制备MePEG-PLA纳米粒对成骨细胞的毒性

王忠磊，高  岩，赖春花，卢海宾，李少冰，蒋  颖，韦丛云，周  磊   

  1. 南方医科大学附属口腔医院，广东省口腔医院，广东省广州市   510280
  • 收稿日期:2011-02-24 修回日期:2011-04-25 出版日期:2011-05-21 发布日期:2011-05-21
  • 通讯作者: 周磊，博士，主任医师，教授，博士生导师，南方医科大学附属口腔医院，广东省口腔医院，广东省广州市510280 zho668@263.net
  • 作者简介:王忠磊★，男，1983年生，山东省烟台市人，南方医科大学在读硕士，主要从事口腔种植临床研究。 wangzhl_2008@sina.com
  • 基金资助:

    国家”十一五”科技支撑计划(2007BAI18BO6)“牙列缺损及缺失的种植修复与功能重建的研究”。

Cytotoxicity effect of MePEG-PLA nanoparticles prepared by modified self-emulsion solvent diffusion technique on osteoblasts in vitro

Wang Zhong-lei, Gao Yan, Lai Chun-hua, Lu Hai-bin, Li Shao-bing, Jiang Ying, Wei Cong-yun, Zhou Lei   

  1. The Affiliated Stomatological Hospital of Southern Medical University & Guangdong Provincial Stomatological Hospital, Guangzhou  510280, Guangdong Province, China
  • Received:2011-02-24 Revised:2011-04-25 Online:2011-05-21 Published:2011-05-21
  • Contact: Zhou Lei, Doctor, Chief physician, Doctoral supervisor, the Affiliated Stomatological Hospital of Southern Medical University & Guangdong Provincial Stomatological Hospital, Guangzhou 510280, Guangdong Province, China zho668@263.net
  • About author:Wang Zhong-lei★, Studying for master’s degree, the Affiliated Stomatological Hospital of Southern Medical University & Guangdong Provincial Stomatological Hospital, Guangzhou 510280, Guangdong Province, China wangzhl_2008@sina.com
  • Supported by:

    the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period, No. 2007BA I18BO6*

PDF

376

被引次数

4

摘要：

背景：两亲性嵌段聚合物由于其较强的载药能力强、纳米级大小、血液中长循环等优点在载药系统中得到广泛的应用。
目的：评估改良自乳化溶剂扩散法制备的甲氧基封端的聚乙二醇-聚乳酸 (MePEG-PLA)纳米粒对人骨肉瘤细胞MG63的毒性。
方法：通过改良自乳化溶剂扩散法制备MePEG-PLA纳米粒，MTS法测定纳米粒培养1，2，3 d后对MG63的毒性。激光粒度分析仪测定纳米颗粒的粒径大小、粒径分布及Zeta电位；透射电镜表征纳米胶束外观形态；酶标仪检测培养1，2，3 d细胞吸光度值。
结果与结论：MePEG-PLA纳米粒的平均粒径为25.7 nm，分布均匀，呈球形，Zeta电位为-8.06 mV，MePEG-PLA毒性为 0级。提示改良自乳化溶剂扩散法制备纳米粒简单易行，制备的纳米粒无毒，具有良好的应用前景。

关键词: 纳米粒, 药物载体, 聚乳酸, 聚乙二醇, 改良自乳化溶剂扩散法

Abstract:

BACKGROUND: Amphiphilic block copolymers have been widely applied in the drug delivery system because of the high capacity of drug carriers, nanoparticles and long circulation time.
OBJECTIVE: To evaluate the cytotoxicity effect of MePEG-PLA nanoparticles prepared by modified self-emulsion solvent diffusion technique on MG63 in vitro.
METHODS: MePEG-PLA nanoparticles were prepared by modified self-emulsion solvent diffusion technique. The cytotoxicity effect of MePEG-PLA nanoparticles on MG63 was detected using MTS assay at days 1, 2, 3. Particles size, polydispersity index and Zeta electric potential were measured by laser particle size analzer. The morphology of MePEG-PLA nanoparticles were observed by transmission electron microscope. Absorbance values at days 1, 2, 3 were detected by microplate reader.
RESULTS AND CONCLUSION: MePEG-PLA nanoparticles had a regular spherical shape with the mean diameter of 25.7 nm and scattered evenly. The Zeta potential was -8.06 mV. The cytotoxicity grade of MePEG-PLA nanoparticles was 0. The results indicate that MePEG-PLA nanoparticles prepared easily by modified self-emulsion solvent diffusion technique are nontoxic to the MG63 and have a good prospect.

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