中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (20): 3785-3788.doi: 10.3969/j.issn.1673-8225.2011.20.043

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

Fas及FasL和Caspase-3在青蒿琥酯诱导人胚肺成纤维细胞凋亡中的表达

王昌明，黎洪秀，张孝飞   

  1. 桂林医学院附属医院呼吸科，广西壮族自治区桂林市  541001
  • 收稿日期:2011-01-06 修回日期:2011-04-09 出版日期:2011-05-14 发布日期:2011-05-14
  • 通讯作者: 黎洪秀，桂林医学院在读硕士，桂林医学院附属医院呼吸科，广西壮族自治区桂林市 541001
  • 作者简介:王昌明☆，1963年生，博士，教授，硕士生导师，主要从事呼吸科疾病的诊治工作。 并列第一作者：黎洪秀，桂林医学院在读硕士，桂林医学院附属医院呼吸科，广西壮族自治区桂林市 541001
  • 基金资助:

    课题受广西科学研究与技术开发计划课题资助(桂科攻10124008-4)。课题名称：青蒿琥酯对肺成纤维细胞增殖影响的分子生物学机制研究。

Role of Fas, FasL and Caspase-3 in artesunate-induced apoptosis of human embryonic lung fibroblasts

Wang Chang-ming, Li Hong-xiu, Zhang Xiao-fei   

  1. Department of Respiration, the Affiliated Hospital of Guilin Medical College, Guilin  541001, Guangxi Zhuang Autonomous Region, China
  • Received:2011-01-06 Revised:2011-04-09 Online:2011-05-14 Published:2011-05-14
  • Contact: Li Hong-xiu, Studying for master’s degree, Department of Respiration, the Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region, China 763460155@qq.com
  • About author:Wang Chang-ming☆, Doctor, Professor, Master’s supervisor, Department of Respiration, the Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region, China wcm@glmc.edu.cn Li Hong-xiu, Studying for master’s degree, Department of Respiration, the Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region, China 763460155@qq.com Wang Chang-ming and Li Hong-xiu contributed equally to this paper.
  • Supported by:

    Science Research and Technology Development Plan of Guangxi Province, No. 10124008-4*

PDF

335

被引次数

11

摘要：

背景: 青蒿琥酯具有减轻肺纤维化的作用，但相关机制的研究罕见报道。
目的：探讨青蒿琥酯对人胚肺成纤维细胞凋亡的作用及其与Fas，FasL，Caspase-3表达的关系。
方法：用1，10，100 mg/L青蒿琥酯分别干预体外培养的人胚肺成纤维细胞。采用CCK-8法检测青蒿琥酯对人胚肺成纤维细胞增殖的影响，流式细胞术测定细胞凋亡率，RT-PCR法测定Fas，FasL，Caspase-3 的mRNA的表达。
结果与结论：青蒿琥酯呈浓度依赖性抑制人胚肺成纤维细胞增殖，细胞经青蒿琥酯作用后凋亡率明显增加(P  < 0.05或P  < 0.01)，Fas，FasL，Caspase-3 mRNA的表达显著高于对照组(P < 0.05)。结果证实，青蒿琥酯可通过上调Fas，FasL，Caspase-3 mRNA的表达抑制人胚肺成纤维细胞增殖、并促进细胞凋亡，发挥抗肺纤维化作用。

关键词: 青蒿琥酯, 人胚肺成纤维细胞, 凋亡, 增殖, 组织工程

Abstract:

BACKGROUND: Artesunate can relieve pulmonary fibrosis, but its mechanisms are rarely reported.
OBJECTIVE: To explore the effect of artesunate on apoptosis of HFL-I cells and the role of Fas, FasL and Caspase-3 in the artesunate-mediated apoptosis of HFL-I cells.
METHODS: Cell counting kit-8 (CCK-8) assay was used to determine the effects of artesunate at 1, 10, 100 mg/L on the growth of HFL-I cells in vitro. Apoptosis ratio was examined by flow cytometry (FCM). The mRNA level of Fas, FasL and Caspase-3, were assessed by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS AND CONCLUSION: Artesunate had a significantly inhibitory effect on the proliferation of HFL-I cells in a dose-dependent manner in vitro. The apoptosis rate of HFL-I cells was significantly increased in the artesunate-treated group compared with the control group (P < 0.05 or P  < 0.01). The mRNA levels of Fas, FasL and Caspase-3 were significantly higher in the artesunate-treated group than the control group (P < 0.05). The findings of this study demonstrate that artesunate can exert a marked anti-pulmonary fibrosis effect by up-regulating mRNA levels of Fas, FasL and Caspase-3, which can induce the growth inhibition and apoptosis in HFL-I cells.

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