中国组织工程研究

中国组织工程研究

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

电针对骶髓损伤后神经源性膀胱容量及其组织形态学的影响

李景兴1，张  泓2，艾  坤2，张雨辰2，匡静之2，鲍秋影2   

  1. 1衡阳市中心医院儿童康复中心，湖南省衡阳市  421000；2湖南中医药大学针灸推拿学院，湖南省长沙市  410208
  • 修回日期:2013-10-17 出版日期:2013-12-10 发布日期:2013-12-10
  • 通讯作者: 张泓，博士，教授，博士研究生导师，湖南中医药大学针灸推拿学院，湖南省长沙市 410007 zh5381271@qq.com
  • 作者简介:李景兴★，男，1983年生，湖南省郴州市人，硕士，医师，主要从事针灸治病机制及儿童成人康复的研究。 283962818@qq.com
  • 基金资助:

    湖南省自然科学基金项目(13JJ3098)*；湖南省中医药管理局重点项目(201109)*；湖南省教育厅科研项目(1200264)*

EIectroacupuncture effects on bladder capacity and tissue morphology of the neurogenical bladder after sacral spinal cord injury

Li Jing-xing1, Zhang Hong2, Ai Kun2, Zhang Yu-chen2, Kuang Jing-zhi2, Bao Qiu-ying2   

  1. 1Rehabilitation Center for Children, Hengyang Central Hospital, Hengyang  421000, Hunan Province, China; 2College of Acupuncture, Moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha  410007, Hunan Province, China
  • Revised:2013-10-17 Online:2013-12-10 Published:2013-12-10
  • Contact: Zhang Hong, M.D., Professor, Doctoral supervisor, College of Acupuncture, Moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China zh5381271@qq.com
  • About author:Li Jing-xing★, Master, Physician, Rehabilitation Center for Children, Hengyang Central Hospital, Hengyang 421000, Hunan Province, China 283962818@qq.com
  • Supported by:

    the Natural Science Foundation of Hunan Province, No. 13JJ3098*; the Major Project of Hunan Provincial Administration of Traditional Chinese Medicine, No. 201109*; the Research Project of Hunan Provincial Education Bureau, No. 1200264*

被引次数

18

摘要：

背景：骶段的脊髓和腰椎病变导致脊髓损伤使骶髓初级排尿中枢受损或其周围神经(副交感和体神经)病变引起逼尿肌无反射，会导致尿潴留，进而导致膀胱组织细胞形态学也发生了病理性变化。
目的：观察电针次髎、中极、三阴交穴对骶髓损伤后尿潴留模型大鼠最大膀胱容量及其组织形态学的影响。
方法：SD雌性大鼠40只，随机抽取10只为空白组，其余制备骶髓损伤模型后随机摸球法均分为模型组、电针穴位组和电针对照点组。模型组大鼠只捆绑不针刺，穴位组取大鼠“次髎”、“中极”、“三阴交”，对照点组取非穴对照点，针刺后加电针，均治疗20 min。且所有大鼠在治疗后第14天和第22天行膀胱容量检测，治疗结束后取膀胱组织行苏木精-伊红染色观察大鼠膀胱组织形态学变化。
结果与结论：①电针穴位组最大膀胱容量较治疗前明显降低(P < 0.01)，并且较对照点组疗效更明显(P < 0.05)，穴位组膀胱容量差值(d值)较对照点组差异更明显(P < 0.05)。②电针穴位组膀胱组织形态学较模型组及对照点组有明显改善。说明电针次髎、中极、三阴交穴可有效降低骶髓损伤后尿潴留大鼠最大膀胱容量并使受损的膀胱组织细胞得到一定程度修复。



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

关键词: 组织构建, 组织构建基础实验, 电针, 骶髓损伤, 尿潴留, 膀胱容量, 膀胱组织形态, 逼尿肌, 神经反射, 省级基金

Abstract:

BACKGROUND: Spinal cord injuries induced by sacral segment and lumbar lesions can damage the primary sacral micturition center or its surrounding nerves (parasympathetic and somatic nerve) to cause detrusor areflexia, thereby leading to urinary retention and further pathological changes in bladder tissue morphology.
OBJECTIVE: To observe the effects of electroacupuncture at the Ciliao (BL32), Zhongji (RN3), Sanyinjiao (SP6) acupoints on bladder capacity and bladder tissue morphology of rats with urinary retention after sacral spinal cord injury.
METHODS: Ten of 40 female Sprague-Dawley rats were randomly selected as blank group, and other 30 rats were randomly divided into model group, acupoint group and non-acupoint group. In the model group, the rats received no electroacupuncture. In the non-acupoint group, acupuncture and electroacupuncture at non-acupoint points were performed, respectively, for 20 minutes. In the acupoint group, acupuncture and electroacupuncture were performed at the Ciliao, Zhongji, and Sanyinjiao acupoints. Bladder capacity was measured at days 14 and 22 after treatment. After treatment, bladder tissues were extracted for morphological observation with hematoxylin-eosin staining.
RESULTS AND CONCLUSION: (1) The maximum bladder capacity in the acupoint group was obviously reduced after treatment (P < 0.01); the curative effect in the acupoint group was more obvious than the non-acupoint group (P < 0.05); the difference between preoperative and postoperative bladder capacity (d value) was more significant in the acupoint group than the non-acupoint group (P < 0.05). (2) Compared with the model and non-acupoint groups, morphological changes in the bladder tissue were improved more significantly in the acupoint group. These findings indicate that electroacupuncture at the Ciliao, Zhongji, and Sanyinjiao acupoints can effectively reduce the maximum bladder capacity and repair the damaged bladder tissue after sacral spinal cord injury.



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

Key words: electroacupuncture, acupuncture points, urinary bladder, spinal cord injuries, urinary retention

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