中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 5011-5015.doi: 10.3969/j.issn.1673-8225.2011.27.016

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

骨髓单个核细胞移植对心肌基质细胞衍生因子1-CXCR4轴的影响

张云强，徐爱国，齐向前   

  1. 天津医科大学心血管临床学院、泰达国际心血管病医院，天津市，300457
  • 收稿日期:2011-03-11 修回日期:2011-05-20 出版日期:2011-07-02 发布日期:2011-07-02
  • 作者简介:张云强☆，男，1975年生，天津市人，汉族，2009年天津医科大学毕业，博士，主治医师，主要从事心血管内科学方面的研究。 zhangyqby@126.com

Influence of autologous bone marrow mononuclear cells transplantation on SDF-1-CXCR4

Zhang Yun-qiang, Xu Ai-guo, Qi Xiang-qian   

  1. Cardiology Clinical Institute of Tianjin Medical University, TEDA International Cardiology and Vascular Hospital, Tianjin  300457, China
  • Received:2011-03-11 Revised:2011-05-20 Online:2011-07-02 Published:2011-07-02
  • About author:Zhang Yun-qiang☆, Doctor, Attending physician, Cardiology Clinical Institute of Tianjin Medical University, TEDA International Cardiology and Vascular Hospital, Tianjin 300457, China zhangyqby@126.com

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被引次数

1

摘要：

背景：心脏干细胞移植后心肌基质细胞衍生因子1-CXCR4轴表达及其作用越来越受到人们的关注。
目的：观察经心外膜注骨髓单个核细胞对心衰犬心脏基质细胞衍生因子1-CXCR4轴 mRNA表达的影响。
方法：16只杂种犬随机数字表法均分为移植组和对照组，植入永久起搏器。右室快速起搏三四周后建立心衰模型。移植组犬经心外膜多点注射骨髓单个核细胞悬液，对照组注射等量生理盐水。
结果与结论：快速起搏三四周后，各项超声参数及血流动力学参数较起搏前改变明显，差异有显著性意义。定量PCR检测细胞移植组基质细胞衍生因子1 mRNA及CXCR4 mRNA表达水平高于对照组(P < 0.01)。说明经心外膜注射的骨髓单个核细胞可提高心肌基质细胞衍生因子1 mRNA及CXCR4 mRNA表达水平。

关键词: 快速右室起搏, 骨髓单个核细胞, 基质细胞衍生因子1, CXCR4, mRNA表达

Abstract:

BACKGROUND: Recently, the SDF-1-CXCR4 mRNA expression and the effect after stem cell transplantation have obtained more attention.
OBJECTIVE: To observe the changes of SDF-1 mRNA and CXCR4 mRNA expression in the myocardium after bone marrow mononuclear cells (BM-MNCs) transplantation.
METHODS: Sixteen mongrel dogs were randomly divided into the graft group and the control group. Each of dog was implanted a permanent pacemaker for rapid right ventricular pacing for 3 to 4 weeks. CM-DiI 1abled BM-MNCs or saline were transplanted into the myocardium via epicardium injection.
RESULTS AND CONCLUSION: After 3-4 weeks of rapid pacing, the cardiac function of the dogs in both two groups significantly decreased in hemodynamic and in ultrasound parameters. The mRNA levels of SDF-1 and CXCR4 were higher in the graft group than those in the control group (P < 0.01). Autologous BM-MNCs transplantation improves the mRNA level of SDF-1 and CXCR4.

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