中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (20): 3733-3736.doi: 10.3969/j.issn.1673-8225.2011.20.029

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

犬切牙压低移动过程中牙周组织骨保护素/核因子κB受体活化因子配体的表达

葛振林1，杨彩霞1，卢嘉静2，祁  涛3，田佳灵1   

  1. 1兰州大学口腔医学院正畸科，甘肃省兰州市730000
    2泰州职业技术学院，江苏省泰州市 225300
    3兰州大学第一医院正畸科，甘肃省兰州市 730000
  • 收稿日期:2011-01-06 修回日期:2011-04-14 出版日期:2011-05-14 发布日期:2011-05-14
  • 作者简介:葛振林，男，汉族，1967年生，甘肃省人，1990年兰州医学院毕业，副教授。主要从事口腔正畸学基础与临床研究。 gezhl@lzu.edu. cn
  • 基金资助:

    课题受甘肃省科技支撑计划项目(090NKCA111)及兰州市科技发展计划项目(2008-1-90)资助。

Expression of osteoprotegerin/receptor activator of nuclear factor kappa B ligand in the periodontal tissue incident to incisors intrusion on dog

Ge Zhen-lin1, Yang Cai-xia1, Lu Jia-jing2, Qi Tao3, Tian Jia-ling1   

  1. 1Department of Orthodontics, School of Stomatology, Lanzhou University, Lanzhou  730000, Gansu Province, China
    2Department of Stomatology, Taizhou Polytechnic College, Taizhou 225300, Jiangsu Province, China
    3Department of Orthodontics, First Hospital of Lanzhou University, Lanzhou  730000, Gansu Province, China
  • Received:2011-01-06 Revised:2011-04-14 Online:2011-05-14 Published:2011-05-14
  • About author:Ge Zhen-lin, Associate professor, Department of Orthodontics, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China gezhl@lzu.edu.cn
  • Supported by:

    the Scientific and Technological Support Project of Gansu Province, No. 090NKCA111*; Technology Development Project of Lanzhou, No. 2008-1-90*

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摘要：

背景：骨保护素及核因子κB受体活化因子配体是调控破骨细胞生成和活化的关键因子，机械力可影响牙周膜细胞和成骨细胞骨保护素及核因子κB受体活化因子配体的表达。
目的：观察犬切牙压低移动过程中牙周组织中骨保护素/核因子κB受体活化因子配体的表达。
方法：采用微型种植体作为支抗，将犬切牙分别施加牵引力1，2，4，12周，并设置对照组进行比较。牵引后切取犬切牙连同牙龈及牙槽骨组织块，制作组织切片进行骨保护素及核因子κB受体活化因子配体免疫组织化学染色，用Image-Proplus软件半定量分析图像平均吸光度值。
结果与结论：与对照组相比，核因子κB受体活化因子配体和骨保护素分别在在施加牵引力1和2周表达最显著，其平均吸光度值在施加牵引力1和2周时可达到峰值(P < 0.05)，随后逐渐下降，施加牵引力12周恢复至对照组水平。结果证实，正畸牙压低移动过程中核因子κB受体活化因子配体及骨保护素的表达变化规律与骨改建过程一致，骨保护素/核因子κB受体活化因子配体系统是牙周组织改建的重要调节因素。

关键词: 正畸牙移动, 骨改建, 骨保护素, 核因子&kappa, B受体活化因子配体, 组织构建

Abstract:

BACKGROUND: Osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) play important role in osteoclast formation and activation. The expression of OPG/RANKL in the periodontal tissues during orthodontic tooth movement is to be studied.
OBJECTIVE: To observe the expression of OPG/RANKL in the periodontal tissue incident to incisors intrusion on dogs.
METHODS: Nine healthy hybrid dogs were selected and randomly divided into 5 groups. The control group contained 1 dog with no force loaded; Applied force for 1 week, 2 weeks, 4 weeks and 12 weeks (4 weeks after activation, then retention for 8 weeks) were the four experimental groups, which were contained 2 dogs respectively. The dog of the control group was killed immediately. Dogs of other groups were killed after the force was applied 1 week, 2 weeks, 4 weeks and 12 weeks. Then the gums and alveolar bone tissues were completely cut to make the specimens. An immunohistochemical staining technique was utilized to detect the expression of OPG/RANKL. The average optical density images were semi-quantitative analyzed by Image-Proplus software to reflect the expression levels of RANKL and OPG in the periodontal tissues in each group.
RESULTS AND CONCLUSIONS: The expression of RANKL in the no force loaded group (the control group) in the periodontal ligament tissues showed weak positive. The expression of RANKL was the most significant after applying force for 1 week. The expression still showed positive, but became weak after applying force for 2 weeks. The expression was decreased significantly after applying force for 4 weeks. The expression was close to the control group after activation 4 weeks then retention for 8 weeks. The average optical density level was measured by image analysis and demonstrated that it reached peak in the 1 week (P < 0.05), then declined gradually. There were significant differences compared with the control group (P < 0.05). Expression of OPG in the no force loaded group (the control group) in the periodontal ligament tissues showed weak positive. The expression of OPG was increased significantly after applying force for 1 week. And in 2 weeks, the expression reached peak. Then the expression was decreased after applying force for 4 weeks. The positive expression regained to the level of the control group after activation 4 weeks then retention for 8 weeks. The average optical density value reached peak in the 2 weeks and it was significant stronger than the control group (P < 0.05). The results confirm that the expression change characters of RANKL and OPG are the same as the reconstruction of alveolar bone. RANKL and OPG participate in the reconstruction of periodontal tissues.

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