中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (20): 3728-3732.doi: 10.3969/j.issn.1673-8225.2011.20.028

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

三七总皂苷干预股骨头缺血性坏死兔成骨细胞护骨素基因的表达 

王大伟，史宝明，李小峰，张  爽，莫  坚，顾国龙，林宝举，马开好，惠宁军   

  1. 广西中医学院附属瑞康医院，广西壮族自治区南宁市  530001
  • 收稿日期:2011-03-10 修回日期:2011-03-19 出版日期:2011-05-14 发布日期:2011-05-14
  • 通讯作者: 史宝明，广西中医学院附属瑞康医院，广西壮族自治区南宁市 530001 26826713@qq. com
  • 作者简介:王大伟，男，1956年生，上海市人，汉族，教授，主任医师，主要从事骨与关节退变与缺血性疾病的防治研究。 wdw1956@yeah.net
  • 基金资助:

    广西卫生厅重点科研课题(重200853) ，三七总皂苷对兔股骨头缺血性坏死成骨细胞护骨素基因表达的影响。

Total saponins of Panax notoginseng effects on mRNA expression of osteoprotegerin in osteoblasts from rabbits with avascular necrosis of the femoral head

Wang Da-wei, Shi Bao-ming, Li Xiao-feng, Zhang Shuang, Mo Jian, Gu Guo-long, Lin Bao-ju, Ma Kai-hao, Hui Ning-jun   

  1. Ruikang Hospital, Guangxi College of Traditional Chinese Medicine, Nanning  530011, Guangxi Zhuang Autonomous Region, China
  • Received:2011-03-10 Revised:2011-03-19 Online:2011-05-14 Published:2011-05-14
  • Contact: Shi Bao-ming, Studying for master’s degree, Ruikang Hospital, Guangxi College of Traditional Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China 26826713@qq.com
  • About author:Wang Da-wei, Professor, Chief physician, Ruikang Hospital, Guangxi College of Traditional Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China wdw1956@yeah.net
  • Supported by:

    Key Science and Technology Program of Guangxi Health Bureau, No. 200853*

PDF

495

摘要：

背景：前期研究已明确三七总皂苷能抑制乙醇诱导的兔骨髓基质干细胞成脂分化。
目的：进一步观察三七总皂苷对兔成骨细胞的增殖和分化以及骨保护素、细胞核因子κB受体活化因子配体mRNA表达的影响。
方法：白酒灌胃4周制备新西兰兔股骨头缺血坏死模型，随机分为生理盐水组、复方骨肽组和三七总皂苷组。通过组织块培养法提取各组兔成骨细胞，检测细胞的分化，骨保护素、细胞核因子κB受体活化因子配体基因表达情况，检测各目的基因杂交信号的强弱。
结果与结论：三七总皂苷组兔碱性磷酸酶活性、钙结节计数及骨保护素、细胞核因子κB受体活化因子配体的表达强度、骨保护素mRNA/细胞核因子κB受体活化因子配体mRNA均高于生理盐水组(P < 0.01)；与复方骨肽组效果接近。证实三七总皂苷能够促进兔成骨细胞的增殖和分化，并能提高成骨细胞的骨保护素mRNA的相对表达量而对其细胞核因子κB受体活化因子配体mRNA有抑制作用。

关键词: 三七总皂苷, 乙醇中毒, 股骨头坏死, 骨细胞, 骨保护素

Abstract:

BACKGROUND: Previous studies have demonstrated that total saponins of Panax notoginseng (PNS) can inhibit the ethanol-induced adipogenic differentiation of rabbit bone marrow stromal cells (BMSCs).
OBJECTIVE: To observe PNS effects on osteoblast proliferation and mRNA expressions of osteoprotegerin (OPG) and receptor activator of nuclear factor κB ligand (RANKL).
METHODS: Rabbit models of alcohol-induced avascular necrosis of the femoral head were divided randomly into saline group, compound bone peptide group, PNS group. Rabbit osteoblasts were extracted for measurement of OPG and RANK mRNA expressions and hybridizing signal intensity.
RESULTS AND CONCLUSION: In the PNS group, alkaline phosphatase activity, calcium nodule count, OPG and RANK mRNA expressions and their ratio were significantly higher than those in the saline group (P < 0.01), and similar to those in the compound bone peptide group. The experiment confirmed that PNS can promote the proliferation and differentiation of rabbit osteoblasts, and can improve the OPG mRNA relative expression in osteoblasts so as to inhibit RANKL mRNA expression.

中图分类号: