中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (8): 1499-1503.doi: 10.3969/j.issn.1673-8225.2010.08.041

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

用复合表面活性剂制备更安全的异种骨移植材料

陈  磊，孙  磊，陶剑峰，江  健，高新生，杰永生，田  伟   

  1. 北京积水潭医院/北京市创伤骨科研究所，北京市  100035
  • 出版日期:2010-02-19 发布日期:2010-02-19
  • 通讯作者: 孙 磊，博士，副研究员，北京积水潭医院/北京市创伤骨库研究所，北京市 100035
  • 作者简介:陈 磊，男，1979年生，北京市人，汉族，2005年中国协和医科大学毕业，技师，主要从事骨组织工程、骨移植相关研究。
  • 基金资助:

    北京市科技新星培养计划资助项目(H013610310113)。

Xenographic bone graft materials safely prepared by compound surfactant

Chen Lei, Sun Lei, Tao Jian-feng, Jiang Jian, Gao Xin-sheng, Jie Yong-sheng, Tian Wei   

  1. Beijing Jishuitan Hospital, Beijing Research Institute of Trauma Orthopedics, Beijing  100035, China
  • Online:2010-02-19 Published:2010-02-19
  • Contact: Sun Lei, Doctor, Associate investigator, Beijing Jishuitan Hospital, Beijing Research Institute of Trauma Orthopedics, Beijing 100035, China dr_sunlei@263.net
  • About author:Chen Lei, Technician, Beijing Jishuitan Hospital, Beijing Research Institute of Trauma Orthopedics, Beijing 100035, China chenlei1979@126.com
  • Supported by:

    Beijing Technology New Star Project, No.HO 13610310113*

PDF

448

被引次数

14

摘要：

背景：表面活性剂脱细胞的效果和骨移植材料的生物安全性与表面活性剂的选择有较大关系。因此，实验采用新型的复合表面活性剂制备脱细胞骨移植材料。
目的：应用新型表面活性剂处理生物源性骨组织，并进行脱细胞效果及生物安全性评价，以期得到一种更安全、可靠的骨植入材料。
方法：2 种阴离子表面活性剂十二烷基苯磺酸钠和脂肪醇聚氧乙烯醚硫酸钠以及蒸馏水以质量比13∶7∶80 的比例配制复合表面活性剂。以新鲜的牛松质骨为原料，复合表面活性剂脱脂脱细胞两步法工艺，制备新型生物源性骨植入材料。
结果与结论：复合表面活性剂生物源性骨颜色呈乳白色，未见杂质，组织学及超微结构观察可见骨陷窝内细胞结构消失，骨小管空虚，胶原纤维排列整齐。生物安全性实验表明：按GB/T16886.11-1997标准急性全身毒性试验合格，溶血试验< 5%，细胞毒性试验0级。复合表面活性剂生物源性骨组织中表面活性剂的残留量低于0.1 g/L。骨长期植入实验表明：植入材料与宿主骨融合良好，24 周后被机体完全吸收。说明复合表面活性剂处理的生物源性骨移植材料具有良好的生物相容性和生物降解性，是一种安全、可靠的骨植入材料。

关键词: 脱细胞, 表面活性剂, 骨移植, 骨移植材料

Abstract:

BACKGROUND: Effect of acellular surfactant and biological safety of bone graft materials highly correlated with selection of surfactant; therefore, a novel compound surfactant was used to prepare acellular bone graft materials in this study.
OBJECTIVE: To evaluate acellular effect and biological safety of bio-derived bone tissue treated by a novel surfactant in order to obtain a safe and reliable bone graft material.
METHODS: Surfactant was prepared with anionic surfactant sodium dodecyl benzene sulfonate (ABS), anionic surfactant sodium fatty alcohol ether sulfate (AES) and distilled water at the ratio of 13:7:80. Fresh bovine cancellous bone and surfactant which was used to remove cells and lipid by two-step flow were used to prepare a novel bio-derived bone graft material. The histological and microscopic observations of microstructure were made. Also acute body toxicity test, hematolysis experiment, cell toxicity test and biological safety were assessed on surfactant-treated bio-derived bone graft material (STBB). A long-term animal experiment was conducted to observe the biocompatibility and biodegradability of STBB. The ultraviolet dispersion of light luminosity method was employed to measure the residual amount of surfactant in STBB.
RESULTS AND CONCLUSION: STBB was a whitish porous cancellous bone. No cell was found in bone lacuna, bone canaliculus was empty, and the collagen fiber had an order arrangement. Acute body toxicity test was qualified according to GB/T16886.11-1997 standard, hematolysis experiment was < 5%, and cell toxicity test was grade 0, confirming that STBB was safe. The remaining surfactant in STBB was lower than 0.1 g/L. The long-term animal experiment demonstrated that fiber was present at 4 weeks, bone lacuna had cellular growth and the fusion of STBB and host appeared. The STBB was partial absorbed by organism at 8 weeks and completely absorbed at 24 hours. The results indicated that STBB had an excellent biocompatibility and biodegradability. As a new bone implant material, STBB was safe and dependable for transplantation.

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