中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (15): 2684-2687.doi: 10.3969/j.issn.1673-8225.2010.15.006

• 骨组织构建 bone tissue construction • 上一篇    下一篇

脊髓完全横断损伤大鼠的骨转换：超早期高压氧干预之效应

童  敏1，伍贤平2，陈  军1，刘  敏3   

  1. 中南大学湘雅二医院，1动物实验室，2代谢内分泌所，3康复科，湖南省长沙市 410011
  • 出版日期:2010-04-09 发布日期:2010-04-09
  • 通讯作者: 刘 敏，硕士，副教授，中南大学湘雅二医院康复科，湖南省长沙市 410011 liumin22626@126.com
  • 作者简介:童 敏★，女，1971年生，湖南省长沙市人，汉族，2008年中南大学毕业，硕士，主管技师，主要从事实验动物药理学的研究。 tongmin22626@163.com
  • 基金资助:

    湖南省科技厅资助项目(2008SK3108)。

Bone turnover in complete spinal cord transaction rats: Influence of ultra-early hyperbaric oxygen treatment 

Tong Min1, Wu Xian-ping2, Chen Jun1, Liu Min3   

  1. 1 Animal Laboratory, 2 Institute of Endocrine and Metabolic Disease, 3 Department of Rehabilitation, Second Xiangya Hospital, Central South University, Changsha   410011, Hunan Province, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Liu Min, Master, Associate professor, Department of Rehabilitation, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China liumin22626@126.com
  • About author:Tong Min★, Master, Technician-in-charge, Animal Laboratory, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China tongmin22626@163.com
  • Supported by:

    the Program of Department of Science and Technology of Hunan Province, No. 2008SK3108*

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摘要：

背景：有文献表明高压氧治疗对不完全脊髓损伤功能恢复有促进作用，且越早效果越好。
目的：进一步探讨超早期高压氧治疗对脊髓完全横断损伤大鼠骨转换的影响。
方法：SD大鼠随机分为假手术组、模型组和高压氧组，假手术组仅行椎板切除术，其余2组均行T10椎板水平脊髓完全横向切断术。高压氧组于术后第3小时置于动物舱内开始高压氧治疗，舱内达到222.92 kPa个绝对大气压，吸氧浓度97%~99%，10 d 1疗程，共3疗程，其中第1疗程2次/d，第2，3疗程1次/d，每疗程间休息6 d。模型组不做高压氧治疗。分别于建立模型后第6周处理，用ELISA法测骨形成指标血清骨特异性碱性磷酸酶及血清骨钙素，骨吸收指标血清Ⅰ型胶原氨基末端肽及尿脱氧吡啶啉的浓度。
结果与结论：高压氧组大鼠血清骨特异性碱性磷酸酶，血清骨钙素值显著高于模型组(P < 0.05)，模型组显著高于假手术组  (P < 0.05)。高压氧组血清Ⅰ型胶原氨基末端肽，尿清脱氧吡啶的值显著低于模型组(P < 0.05)，模型组显著高于假手术组(P < 0.05)。结果表明超早期高压氧治疗可影响脊髓完全横断损伤大鼠骨转换的改变，对骨形成有促进作用，对骨吸收有抑制作用。

关键词: 高压氧, 脊髓损伤, 骨转换, 碱性磷酸酶, Ⅰ型胶原氨基末端肽, 血清骨钙素

Abstract:

BACKGROUND: Hyperbaric oxygen (HBO) treatment can promote the recovery of incomplete spinal cord injury, and the earlier, the better was.
OBJECTIVE: To explore the influence of ultra-early HBO treatment on bone turnover in complete spinal cord transaction rats.
METHODS: Sprague Dawley rats were randomly divided into sham operation, model and HBO groups. Rats in sham operation group underwent laminectomy, while rats in the other 2 groups underwent complete spinal cord transection at the level of the tenth thoracic vertebra. Rats in HBO group were put in animal oxygen chamber at 3 hours after operation, 222.92 kPa atmosphaera absolutus, oxygen concentration 97%-99%, 10 days as 1 course of treatment, for 3 courses, twice a day in first course, once a day in second and third courses, respectively, with a 6 days’ interval between courses. Model group did not receive HBO treatment. At 6 weeks after model estahblishment, the level of the serum bone alkaline phosphatase (BAP), serum osteocalcin (sOC), serum cross-linked N-telopeptide of collagen type Ⅰ(sNTX) and urinary deoxypyridinoline (DPD) were measured by enzyme linked immunosorbent assay.
RESULTS AND CONCLUSION: The levels of BAP and sOC of HBO group was much higher than that of the model group (P < 0.05). The level of BAP and sOC of the model group was much higher than that of the sham operation group (P < 0.05). The level of sNTX and urine DPD of HBO group was much lower than that of the model group (P < 0.05). The level of sNTX and urine DPD of the model group was much higher than that of the sham operation group (P < 0.05). These suggested that ultra-early HBO treatment affects bone turnover in rats with complete spinal cord injury, contributes to bone formation, and inhibits bone resorption.

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