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    19 November 2011, Volume 15 Issue 47 Previous Issue    Next Issue
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    Choukroun’s platelet-rich fibrin combined with autogenic morselized bone promotes rabbit skull regeneration
    Wang De-li, Kong Xiang-pan, Luan Hai-rong, Lu Shan, Shang Wei-rong
    2011, 15 (47):  8741-8744.  doi: 10.3969/j.issn.1673-8225.2011.47.001
    Abstract ( 281 )   PDF (1298KB) ( 590 )   Save

    BACKGROUND: Autogenic morselized bone is an ideal substitute for tissue engineered stent. Choukroun’s platelet-rich fibrin (PRF) can enhance the bone regeneration. Whether the combination of the two materials can achieve a better effect need to be explored.
    OBJECTIVE: To investigate the effect of Choukroun’s PRF combined with autogenic morselized bone in repairing skull bone defects.
    METHODS: Two standard defects were made by grinded osteotomy on each animal’s skull and then Choukroun’s PRF combined with autogenic morselized bone were used to filled the defect sites on one side as the experimental group and Choukroun’s PRF alone was used on the another side as control group. At postoperatively 4, 6, and 8 weeks, soft X-ray and histological staining were applied.
    RESULTS AND CONCLUSION: The new bone area was significantly larger in the experimental group than in the control group as well as bone healing, bone trabacula density and shape, volume and structure of new bone and number of osteoblasts (P < 0.05 or P < 0.01). It is indicated that Choukroun’s PRF combined with autogenic morselized bone can significantly improve the repair of bone defects superior to Choukroun’s PRF alone.

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    Antibacterial property and biocompatibility of digital silver loaded hydroxyapatite as artificial bone
    Zhu Hui, Yin Qing-shui, Zhang Yu, Zhang Yu, Zhao Hua-fu, Zhou Ye
    2011, 15 (47):  8745-8748.  doi: 10.3969/j.issn.1673-8225.2011.47.002
    Abstract ( 308 )   PDF (1565KB) ( 565 )   Save

    BACKGROUND: The silver loaded coral hydroxyapatite (CHA-Ag) has received increasing attention as a new type of antibacterial bone graft material. Implant materials need well biocompatibility with human body.
    OBJECTIVE: To observe the antibacterial property and biocompatibility of digital CHA-Ag as artificial bone.
    METHODS: The coral hydroxyapatite powder was immersed in silver nitrate solution (AgNO3) with different concentrations in order to prepare CHA-Ag containing different contents of Ag+. Then the prepared CHA-Ag was mixed with poly lactic acid and burned by selective laser to prepare digital artificial bone material containing silver with special shape rapidly.
    RESULTS AND CONCLUSION: The continuum source atomic absorption spectrometer showed that the Ag+ contents of CHA-Ag prepared with 10-2, 10-3, 10-4 and 10-5 mol/L AgNO3 were 2.31×10-1%, 3.18×10-2%, 6.75×10-3%, and 6.05×10-4% respectively. The inhibition ring test demonstrated that the inhibition ring diameter of CHA-Ag by 10-2 mol/L AgNO3 against staphylococcus aureus and Escherichia coli were (13.00±1.52) mm and (12.30 ±1.65) mm respectively; while that of 10-3 mol/L were (11.50±0.73) mm and (11.00±0.4) mm respectively, and no inhibition ring occurred in that of 10-4 and 10-5 mol/L AgNO3. Cytotoxicity test manifested that the cytotoxicity grades were 3, 1, 0 and 0 respectively according to the CHA-Ag prepared with 10-2, 10-3, 10-4 and 10-5 mol/L AgNO3. General toxicity test indicated that the artificial bone leaching liquor with 10-3 mol/L AgNO3 had no obvious acute cytotoxicity in mice. The CHA-Ag prepared with 10-3 mol/L AgNO3 has good biocompatibility and biological security, can inhibit the growth of staphylococcus aureus and Escherichia coli in vitro.

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    Biocompatibility of xenogeneic bone materials prepared by different ways
    Xu Hui-fen, He Hui-yu, Tang Xiao-xue, Cui Jie
    2011, 15 (47):  8749-8752.  doi: 10.3969/j.issn.1673-8225.2011.47.003
    Abstract ( 306 )   PDF (1437KB) ( 615 )   Save

    BACKGROUND: Xenogeneic bone morphology, structure and composition are similar to human bone tissue, and the antigenicity of xenogeneic bone can be decreased after physicochemical treatment, with a natural porous structure. Xenogeneic bone is considered to be an effective way to solve the shortage of the autogenous bone and allograft bone.
    OBJECTIVE: To detect the biocompatibility of xenogenic bone materials prepared by three different ways.
    METHODS: Three sheep vertebral bone xenograft materials were prepared through physical, chemical and physical combined with chemical methods, and they were made extracted liquid and transplanted into the New Zealand white rabbits in vivo. Acute toxicity test, pyrogen test, intramuscular stimulation test and cytotoxicity test were used to evaluate the biocompatibility to sheep vertebral bone xenograft materials of different treatment groups.
    RESULTS AND CONCLUSION: There was no acute toxicity, pyrogen reaction, irritation reaction or cytotoxicity of sheep vertebral bone xenograft materials prepared through physical, physical combined with chemical methods; While there was acute toxicity, pyrogen reaction, mild irritation and cytotoxicity of sheep vertebral bone xenograft materials prepared through chemical methods. The xenogeneic bone materials prepared in the study showed the good biocompatibility through physical, physical combined with chemical methods. However, xenogeneic bone materials prepared through chemical methods, which had poor biocompatibility, could not qualify for the safety standards of the biological materials.

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    Matching ratio of beta tricalcium phosphate/calcium polyphosphate fiber/polylactic acid composite scaffolds for cartilage tissue engineering
    Wang Yan-ping, Zhu Ling-yun, Zhang Hong-mei
    2011, 15 (47):  8753-8756.  doi: 10.3969/j.issn.1673-8225.2011.47.004
    Abstract ( 306 )   PDF (1237KB) ( 337 )   Save

    BACKGROUND: Nowadays, conventional polylactic acid scaffolds have many disadvantages, such as low stiffness, slow degradation, acid degradation solution and induced aseptic inflammation reaction.
    OBJECTIVE: Based on previous study, to optimize the matching ratio of β-tricalcium phosphate/calcium polyphosphate fiber/ polylactic acid scaffolds.
    METHODS: Taking β-tricalcium phosphate and calcium polyphosphate fiber as reinforced materials and polylactic acid as matrix materials in a ratio of 20/30/50, the composite scaffolds for cartilage tissue engineering were fabricated by solvent-casting particulate-leaching and gas foam methods, and their physical and mechanical properties and degradation rate and microstructure were tested.
    RESULTS AND CONCLUSION: The scaffolds with porosity of 70%-95% possessed three-dimensional, interconnecting, micro-hole network structure; Compared with comparable polylactic acid scaffolds, the scaffolds had higher compressive modulus; The degradation rate of scaffolds could be controlled by adding fiber and adjusting porosity of scaffolds; Adding β-tricalcium phosphate could make the pH value of degradation solution maintain at 6.0 to 7.0 and avoid aseptic inflammation reaction caused by acidic degradation products. Therefore, the physical and mechanical properties and degradation rate of the composite scaffolds can meet the demand of cartilage tissue engineering.

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    Preparation of surface-patterned biodegradable poly(organophosphazene) and its affinity to osteoblasts
    Cai Qing, Qi Bing, Duan Shun, Mao Ji-fu, Shen Hong, Yang Fei, Yang Xiao-ping, Wang Shen-guo
    2011, 15 (47):  8757-8761.  doi: 10.3969/j.issn.1673-8225.2011.47.005
    Abstract ( 298 )   PDF (1606KB) ( 403 )   Save

    BACKGROUND: It has been identified that surface topographical structures in nano- or micro-scale can significantly affect the adhesion and proliferation of cells on artificial matrixes, and promote cell behaviors.   
    OBJECTIVE: To investigate the effect of preparation parameters on the surface characteristics of poly(glycine ethyl ester-co- alaine ethyl ester)phosphazene (PGAP) films, and the osteoblasts behaviors on PGAP films.  
    METHODS: PGAP was dissolved in chloroform and polymeric solutions with different concentrations were obtained. Then the solutions were cast onto PTFE plates under relative humidity (RH) of 20% or 80% at 25 ℃. After 2 hours of solvent evaporation, the samples were vacuum-dried at 25 ℃ to constant weight. The resulted films were characterized with scanning electron microscope, atomic force microscope, and X-ray diffraction and water contact angle measurement to determine their surface morphology, composition and hydrophilicity. Mouse osteoblast-like cells OCT-1 were seeded onto the PGAP films, and the effect of surface features of PGAP films on cell behaviors were evaluated via determination of cell adhesion efficiency, proliferation and morphology.  
    RESULTS AND CONCLUSION: The surface morphology and composition of solvent-cast PGAP films depended closely on the solution concentration and environmental RH. In comparison with smooth PGAP films prepared under low RH, surface-patterned PGAP films were obtained under high RH which contributed high surface roughness. The porous structure turned more regular as the solution concentration increasing, a kind of honeycomb structure was observed at the concentration of 120 g/L. However, the surface pattern was blurred when the solution concentration was further increased to 160 g/L, owing to the increased viscosity and the slowed solvent evaporation. Accumulation of polar phosphorous and nitrogen atoms on film surface has been found in the cases of PGAP films prepared under RH of 80%, which resulted in high protein absorption ability in combination with the increased surface roughness. These features facilitated the adhesion, spread and proliferation of mouse osteoblast-like cells OCT-1. All the results suggested that parameters like RH and solution concentration could be an easy way to alter the surface properties of PGAP films and thus could be used in the study of cell-matrix interaction.

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    Expression of integrin alpha 5 beta 1 during the adhesion of osteoblasts to bio-derived materials
    Qi Jie, Zhang Shu-jiang, Liu Zong-zhi
    2011, 15 (47):  8762-8764.  doi: 10.3969/j.issn.1673-8225.2011.47.006
    Abstract ( 287 )   PDF (1273KB) ( 347 )   Save

    BACKGROUND: Integrin plays an important role in the cell adhesion process.
    OBJECTIVE: To explore the expressions and function of integrinα5β1 during the adhesion of osteoblasts to bio-derived materials.
    METHODS: Fetal osteoblasts as seed cells were used to construct tissue engineered bone and periosteum following incubation on the bio-derived materials, which were cultured for 2, 4, 6, 8, 10 days in vitro. Osteoblasts cultured alone served as controls.
    RESULTS AND CONCLUSION: Integrinα5 did not express at the early-stage. Integrinβ1 kept expressing in all groups, and expressed higher on bio-derived bone than on tissue-engineered periosteum, especially at the 2nd and 6th days. Integrinβ1 expressed stably in the control group. The bio-derived materials promote osteoblasts adhesion but fibronectin is not essential to the adhesion of osteoblasts.

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    Complications occurrence at 6 months to 8 years following augmentation mammoplasty with silicone gel implants in 19 cases
    Wang Lu, Liu Lin-bo, Chen Min-jing
    2011, 15 (47):  8765-8768.  doi: 10.3969/j.issn.1673-8225.2011.47.007
    Abstract ( 298 )   PDF (1415KB) ( 451 )   Save

    BACKGROUND: There are many therapies for correction of complications following augmentation mammoplasty with silicone gel implants.
    OBJECTIVE: To observe the effect of second augmentation mammoplasty on patients with complications following augmentation mammoplasty with silicone gel implants.
    METHODS: Nineteen patients with displacement of implants, breasts sagging or capsular contracture at 6 months to 8 years secondary to augmentation mammoplasty with silicone gel implants were selected from the Department of Plastic Surgery of the First Affiliated Hospital of Zhengzhou University from September 2005 to September 2009. The silicone prosthesis was placed into the interspace of the pectoralis major from a specially designed incision, and 50 mg triamcinolone acetonide acetate injection and 160 mg gentamycin sulfate injection were injected into the cavity in one stage.
    RESULTS AND CONCLUSION: All the deformations of 19 cases disappeared at 8-10 weeks, and displacement of implants, breasts sagging and capsular contracture were not observed. All the 19 cases were followed up for over 6 months, and the effects were satisfactory.

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    In vitro construction of decellularized vascular xenograft by improved enzymatic method
    Wu Xin, Gu Yong-quan, Duan Hong-yong, Chen Bing, Li Jian-xin, Wu Ying-feng, Zhang Shu-wen, Wang Zhong-gao, Zhang Jian
    2011, 15 (47):  8769-8772.  doi: 10.3969/j.issn.1673-8225.2011.47.008
    Abstract ( 283 )   PDF (1389KB) ( 414 )   Save

    BACKGROUND: How to expand the pore size and porosity of the acellular matrix to facilitate the post-cell re-cultivation requirement, and simultaneously the graft must ensure its mechanical requirements, is one of the hotspots in vascular tissue engineering.
    OBJECTIVE: To observe the mechanical properties and histocompatibility of decellularized vascular xenograft vascular grafts constructed by the improved enzymatic method.
    METHODS: Porcine carotid arteries were decellularized using conventional trypsin-EDTA plus 1% TritonX-100 and 0.1% ammonia in order for 4, 5, and 6 hours.
    RESULTS AND CONCLUSION: Porcine carotid arteries were decellularized by trypsin, ammonium hydroxide and non-ionic detergent to extract all the cell components of the grafts successfully. To obtain better pore size and porosity of the acellular scaffold, we changed the methods by extending the time of trypsin. By extending the time of trypsin methods, the better pore size and porosity of the acellular scaffold was obtained. Biomechanics, porosity characteristics of scaffold can meet the requirements of tissue engineered vessel.

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    Fibrin glue combined with bone marrow mesenchymal stem cells to repair multi-layer soft tissue defects in rabbits
    Xie Fu-rong, Lin Chun-bo, Yan Dong-xue, Yang Yuan
    2011, 15 (47):  8773-8776.  doi: 10.3969/j.issn.1673-8225.2011.47.009
    Abstract ( 285 )   PDF (1300KB) ( 349 )   Save

    BACKGROUND: Fibrin glue meeting the physical structure of the soft tissue is appropriately used as a carrier material to repair soft tissue defects.
    OBJECTIVE: To investigate the feasibility of bone marrow mesenchymal stem cells combined with fibrin glue to repair soft tissue defects.
    METHODS: Soft tissue defects were made on the deep thigh muscle layer of New Zealand white rabbits with diameter greater than 3 cm. Twenty-four model rabbits were randomly divided into three groups: in experimental group, allogeneic bone marrow mesenchymal stem cells combined with fibrin glue were injected into the soft tissue defects; in control group, the fibrin glue was injected into soft tissue defects of rabbits; in model group, nothing was injected into soft tissue defects of rabbits.
    RESULTS AND CONCLUSION: Wound contraction rates at 14 and 21 days after operation were the experimental group > control group > the model group (P < 0.01); the healing time was the experimental group < control group < model group (P < 0.01). The results showed that the fibrin glue combined with bone marrow mesenchymal stem cells can rapidly repair multi-layer soft tissue defects.

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    Role of adipose-derived mesenchynal stem cell combined with silk fibroin scaffold in enhancing vascular formation in rabbit urethral repair
    Zhang Ya, Zhou Yun, Jia Li-shan, Zhai Jing-mei, Miao Xiao-fen, Wang Rui
    2011, 15 (47):  8777-8781.  doi: 10.3969/j.issn.1673-8225.2011.47.010
    Abstract ( 291 )   PDF (1307KB) ( 383 )   Save

    BACKGROUND: We searched the relevant research papers on main domestic and foreign online retrieval databases in recent five years, and no publication was found on the uretral defect repair with adipose-derived mesenchymal stem cells (ADMSCs) on silk fibroin scaffold.
    OBJECTIVE: To study the effect of ADMSCs combined with a porous silk fibroin scaffold (PSFS) on enhancing vascular formation in rabbit urethral repair.
    METHODS: The ADMSCs were separated from the epididymal fat pads of the rabbits, and then treated with cell isolation and culture. In animal study, the rabbits were assigned into group A with the urethral defects, group B with urethral defects repaired by PSFS, and group C with urethral defects repaired by ADMSCs seeded on PSFS.
    RESULTS AND CONCLUSION: The expression of CD44 and Vimentin was positive on ADMSCs of third passage by indirect immunoflurescence methods. On histological examination, blood vessel formation in group C was significantly more abundant than groups A and B in the 2nd to 6th week after operation. The FⅧ-RAg positive microvessel density was also significantly more than groups A and B in the 4th week after operation (P < 0.05). ADMSCs combined with silk fibroin scaffold can promote in vivo vascularization for urethral repair.

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    Inflammatory response evaluation for electrospun polycaprolactone/silk sericin membranes in vivo
    Lü Yong-gang, Lin Chong-wen, Li Lin-hao, Qian Yu-na, Zhang Xiao-mei, Yang Li, He Jian-hua
    2011, 15 (47):  8782-8786.  doi: 10.3969/j.issn.1673-8225.2011.47.011
    Abstract ( 358 )   PDF (1445KB) ( 413 )   Save

    BACKGROUND: Studies have shown that silk sericin (SS) can accelerate the proliferation of mammalian cells and promote wound healing. SS has good activities for antibacteria, antioxidation and anticancer. SS also has a good biocompatibility and biodegradability. It is an ideal material for tissue engineering, however, there is still a controversy of whether SS causes inflammatory response so far.
    OBJECTIVE: To evaluate the inflammatory response of electrospun polycaprolactone (PCL)/SS membranes.
    METODS: Materials with different PCL/SS ratios were implanted into the back muscles of rats and were taken out after 4 weeks. The inflammatory response was evaluated by hematoxylin eosin staining and macrophage antibody CD68 immunohistochemistry.
    RESULTS AND CONCLUSION: The inflammatory response of the 7:3 group of PCL/SS membranes is similar to that of silk fibroin membrane and was less than those of the 6:4, 5:5 and PCL groups. The 6:4 and 5:5 groups have similar inflammatory response with PCL group. Electrospun PCL/SS membranes can not cause significant inflammatory response. A little amount of SS can decrease the inflammatory response of PCL membrane. However, the inflammatory response of PCL/SS membrane can increase as the ratio of SS to PCL increased.

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    Histological study of enhancing the healing of anterior cruciate ligament autogenous graft with gene activated matrix materials
    Qin Jian, Hou Zhi-qi, Wang Xin-liang, Liu Yong-yi, Gao Xing-hua, Lin Yu-jin, Huang Bao-hua
    2011, 15 (47):  8787-8791.  doi: 10.3969/j.issn.1673-8225.2011.47.012
    Abstract ( 258 )   PDF (1664KB) ( 508 )   Save

    BACKGROUND: The basic concept of gene activated matrix (GAM) is a complex of biological materials and DNA plasmids to form a local gene delivery system.
    OBJECTIVE: To learn the histological changes of rabbit anterior cruciate ligament reconstruction model treated with GAM materials, and to determine the difference in collagen express and miscrostrcture observation.
    METHODS: Forty-eight New Zealand white rabbits were divided into two groups: experiment group and control group. The rabbit anterior cruciate ligament reconstruction model was established according to the previous method. In the experimental group, transforming growth factor β1 was locally injected in the bone tunnel but in the control group the wound was injected with blank plasmids.
    RESULTS AND CONCLUSION: By light miscroscopy the number of fibroblasts in the control group was less than that in the experimental group, and the fibers arranged irregularly at different periods. In the experimental group, the number and appearance of fibroblasts were similar to normal strcture. There were more fibroblasts and collagens which became larger and arranged regularly. By electron miscroscopy we found the karyokinesis of cell miscrostructure was more active and some fibroblasts had a vibrant metabolism in the experimental group. There were plenty of endoplasm and mitochondria in cytoplast and more extracellularmatrix was filled in fibers. The findings indicate that transforming growth factor β1 can enhance the healing of reconstrctured ligament.

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    Adhesiveness and biocompatibility of plant adhesive
    Yu Xue-song, Huang Chi-bing, Zhang Yin-fu, Fan Ming-qi, Wang Ping-xian, Feng Jia-yu, Xiao Ya
    2011, 15 (47):  8792-8796.  doi: 10.3969/j.issn.1673-8225.2011.47.013
    Abstract ( 459 )   PDF (1274KB) ( 430 )   Save

    BACKGROUND: Previous studies have shown that an absorbable hemostatic adhesive with strong adhesiveness was prepared with raw materials extracted from plants.
    OBJECTIVE: To detect the adhesiveness of plant adhesive on kidney and analyze its biocompatibility.
    METHODS: The plant adhesive was diluted into different concentration and used on the surface of rabbit kidney. The adhesiveness in the same concentration was recorded. KM mice were divided into experimental group with the plant adhesive and control group without the plant adhesive.
    RESULTS AND CONCLUSION: There were different adhesion forces with different concentrations of plant adhesive. When 0.6 mL distilled water was added to 1 g plant adhesive, the mixture had good adhesiveness. The incisions of the mice were well-healed after plant adhesive implantation, and there were no difference in blood biochemistry and immunology examinations between the experiment and control groups (P > 0.05). The histopathological staining of the experiment group did not show any inflammatory reaction in incision muscles, liver and kidney tissues. These findings indicate that the plant adhesive has good adhesiveness and biocompatibility.

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    Growth and differentiation of endothelial progenitor cells on the bladder extracellular matrix
    Xiao An-ge, Zhao Kai-liang, Yang Si-xing, Li Yong-wei, Liao Wen-biao, Meng Ling-chao
    2011, 15 (47):  8797-8800.  doi: 10.3969/j.issn.1673-8225.2011.47.014
    Abstract ( 270 )   PDF (1123KB) ( 295 )   Save

    BACKGROUND: Traditional bladder tissue engineering scaffold materials have no vascular structures, and insufficient vascularization is the problem after implantation in vivo.
    OBJECTIVE: To evaluate the biocompatibility of bladder extracellular matrix with endothelial progenitor cells of rabbits.
    METHODS: Rabbit’s endothelial progenitor cells were isolated and cultured. The endothelial progenitor cells combined with the bladder extracellular matrix were cultured and seeded in vitro. The histocompatibility of the complex was evaluated after implantation into the rabbit back.
    RESULTS AND CONCLUSION: Endothelial progenitor cells were able to adhere, grow, and proliferate on the bladder extracellular matrix surface, showing a good cell shape. At 1 week after the endothelial progenitor cells combined with the bladder extracellular matrix were implanted in rabbits, the distinctive inflammatory reaction around the material could be seen, with severe adhesion and mass bleeding. The hematoxylin-eosin staining showed many inflammatory cells infiltrating tissues, collagen and loose arrangement of elastic fibers. After 8 weeks, the implanted materials had been degraded to be broken filaments, fused with the surrounding tissue, with crisp texture and easy bleeding; the hematoxylin-eosin staining showed no obvious tissue infiltration of inflammatory cells in response, closely arranged collagen and elastic fibers and neovasculature could be seen in the materials. The results indicate that the endothelial progenitor cells have a good compatibility with the bladder extracellular matrix, and the complex has a good compatibility with body tissues.

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    Preparation and biological safety of basic fibroblast growth factor/double-layered collagen composite
    Wang Jian-hua, Li Xue-min, Tao Xiao-jun, Chen Hui-juan, Shi Jia-wei, Liu Ling-rong, Zhang Qi-qing
    2011, 15 (47):  8801-8804.  doi: 10.3969/j.issn.1673-8225.2011.47.015
    Abstract ( 303 )   PDF (1352KB) ( 442 )   Save

    BACKGROUND: Collagen is a common used scaffold in tissue engineering, its specific molecular structure and biological activity are conducive to a variety of cell adhesion, proliferation and differentiation, and can speed up wound healing and degradation to provide sufficient space for new tissues.
    OBJECTIVE: To prepare a kind of inhomogeneous double layered collagen composite incorporated with basic fibroblast growth factor (bFGF) loaded chitosan-heparin (CS-Hep) nanoparticles, and to evaluate its biological safety.
    METHODS: The air-dried dense layer collagen membranes and freeze-dried loose layer collagen membrane were prepared respectively and then cross-linked by D-ribose (termed as CAM and CFM respectively). CS-Hep nanoparticles were prepared and then dropped on the surface of CFM. The wet CAM was laid on the surface of CFM with nanoparticles, and then air dried completely at 4 ℃. The obtained material was termed as bDM. Its physical and chemical properties were investigated. In addition, its bio-safety was also examined through acute systemic toxicity, pyrogen, hemolysis and cellular toxicity tests.
    RESULTS AND CONCLUSION: The bFGF/double-layered collagen composite was made up of double layers with one dense layer (CAM) and another loose layer (CFM). As part of bDM, CS-Hep nanopartiles with irregular global shape were aligned in the scope of D-period structure of the inner surface of CFM; the bFGF/double-layered collagen composite had no systemic acute toxicity, no cytotoxicity and pyrogen reactions as well as no hemolytic effect. The bFGF/double-layered collagen composite is prepared and found to have good biocompatibility and safety. Moreover, this material conforms to the ISO 10993-1, and can be used as a basic scaffold material in tissue engineering.

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    Preparation, structure and properties of HAF/YSZ gradient composite coatings
    Li Su-min, Zhang Qin, Zhao Yu-tao, Da Zu-lin, Liu Xiao-yan, Zhang Zhao
    2011, 15 (47):  8805-8808.  doi: 10.3969/j.issn.1673-8225.2011.47.016
    Abstract ( 379 )   PDF (1504KB) ( 604 )   Save

    BACKGROUND: Plasma spraying, sol-gel coating process and ion beam sputtering are often used for making coatings on the surface of titanium alloy, but the properties of the coatings are unstable, the composition is homogeneous, and the control of the phase composition is difficult.
    OBJECTIVE: To prepare HAF/YSZ gradient composite coatings on Ti6Al4V substrate by radio frequency magnetron sputtering.
    METHODS: The HAF/YSZ gradient composite coatings were deposited on Ti6Al4V substrate by radio frequency magnetron sputtering. X-ray photoelectron spectrum, electron probe, and scanning electron microscope were used to investigate the composition distribution, surface morphology and interface morphology of the coatings. The biological activity and stability of HAF/YSZ coatings, HAF1.2 coatings and HA coatings were analyzed and evaluated in siulated body fluid.
    RESULTS AND CONCLUSION: The prepared HAF/YSZ gradient coatings could provide well grow conditions for fresh osseous tissue based on their porous structure and rough surface. The interface was closely combined with the coatings. Interdiffusion existed between layers, and the overall consistency was better. New substances deposited on the surfaces after immersing in siulated body fluid, which showed better biological activity and stability of HAF/YSZ gradient coatings. The anti-solubility and stability of HAF/YSZ coatings were better than that of single fluorine content coatings.

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    Histocompatibility of implantable glucose sensor membrane materials: Comparison of chitosan and Nafion
    Liu Bo-ji, Sha Xian-zheng
    2011, 15 (47):  8809-8812.  doi: 10.3969/j.issn.1673-8225.2011.47.017
    Abstract ( 401 )   PDF (1943KB) ( 568 )   Save

    BACKGROUND: Chitosan is a natural polysaccharide polymer, and can be applied alone or combined with other materials to prepare dressings, drugs, gene vectors, biological coatings, tissue engineering scaffolds, sensors membranes, etc.
    OBJECTIVE: To determine the soft-tissue histocompatibility of chitosan (CS) and Nafion that can be used to optimize the properties of implantable glucose sensors.
    METHODS: CS membrane was prepared, characterized, and subcutaneously implanted to investigate the biodegradation property in vivo. Muscularly implanted in mice, the histocompatibility of CS was compared with common sensor material Nafion.
    RESULTS AND CONCLUSION: The properties of CS membrane could be controlled through preparation techniques. Compared with muscularly implanted, CS membrane subcutaneously implanted resulted in heavier inflammatory reaction and the average degradation rate was (17.0±9.9)% at 63 days after subcutaneously implanted. The fibrous capsule (FC) thickness was comparable with no statistic difference (P > 0.05) for CS and Nafion, and both became stable after 15 days. Compared with muscularly implanted, FC thickness of CS subcutaneously implanted was thicker. As a result, it is proved that CS and Nafion are both biocompatible to be used in implantable glucose sensor.

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    Preparation and biocompatibility of negative pressure adsorption hemostasis application
    Li Ming-yang, Huang Chi-bing, Fan Ming-qi, Feng Jia-yu, Zhang Jia-xi, Gao Yu, Chen Yi-rong
    2011, 15 (47):  8813-8816.  doi: 10.3969/j.issn.1673-8225.2011.47.018
    Abstract ( 325 )   PDF (1368KB) ( 386 )   Save

    BACKGROUND: Some hemostatic materials applied at present have many shortages, such as toxicity and high cost.
    OBJECTIVE: To study the biocompatibility of negative pressure adsorption hemostasis application in animals.
    METHODS: A strip-shaped incision was created in the liver and kidney, 1 cm in length and 0.5 cm in depth. Negative pressure adsorption hemostasis application was used on the wound area of six healthy pigs. General state of the healthy pigs was observed before and after the operation. Blood routine examination, liver and kidney function, body fluid immunology before and 14 days after the operation were detected. Histopathological changes at 14 days were observed.
    RESULTS AND CONCLUSION: There were no significant differences in blood biochemistry and immunology between the two groups (P > 0.05) and the histopathological results did not show any inflammatory reaction in incisions of liver and kidney tissues. There was no inflammation or fibrous tissue envelope. Negative pressure adsorption hemostasis application has good biocompatibility without any toxicity, reject reaction, immunological reaction and anaphylactic response in animals.

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    Effect of surface treatment on the hemocompatibility of Ni-Ti shape memory alloy
    Lu Tan, Tian Bo, Zhang Jiang-feng, Wu Da-peng, Xu Hai-bin
    2011, 15 (47):  8817-8820.  doi: 10.3969/j.issn.1673-8225.2011.47.019
    Abstract ( 351 )   PDF (1400KB) ( 617 )   Save

    BACKGROUND: Implantable Ni-Ti shape memory alloys must have better biocompatibility and biological security.
    OBJECTIVE: To investigate the effect of surface treatment on the biocompatibility of Ni-Ti shape memory alloy.
    METHODS: Ni-Ti shape memory alloy was randomly divided into two groups: experiment group’s materials were surface-treated by cathode electrodeposition; the others not treated were belonged to blank group. We observed the changes of material surface by scanning electron microscope and evaluated the hemolysis rate and kinetic coagulation time. The bone marrow stromal stem cells were cultured with two groups’ materials in vitro, and then we evaluated the MTT value of the two groups.
    RESULTS AND CONCLUSION: After surface treatment of Ti-Ni shape memory alloy, there were many nano-size particles on its surface that formatting Ti-O. In the experimental group, the hemolysis rate was decreased and the kinetic coagulation time was prolonged. The MTT values in the experimental group were significantly higher than those in the blank group at the 2nd, 4th and 6th days after co-culture (P < 0.05). Ni-Ti shape memory alloy treated by cathode electrodeposition has the better biocompatibility and biological security.

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    Preparation of three-dimensional silk fibroin/chitosan scaffolds
    She Rong-feng, Deng Jiang, Huang Wen-liang, Yuan Cheng, Dong Zhi-jun
    2011, 15 (47):  8821-8824.  doi: 10.3969/j.issn.1673-8225.2011.47.020
    Abstract ( 324 )   PDF (1298KB) ( 673 )   Save

    BACKGROUND: Many studies have shown that silk fibroin (SF) and chitosan (CS) as natural polymer materials are non-toxic and tasteless, and they have good physical and chemical properties and biological characteristics.
    OBJECTIVE: To discuss the preparation methods of three-dimensional SF/CS scaffolds which meet the requirements of cartilage tissue engineering scaffolds.
    METHODS: SF and CS were mixed at different ratios such as 3 to 1, 1 to 1, 1 to 3 and 0 to 3, then to make composite materials. In order to find out the best mixing ratio of SF and CS, the following indexes including pore size, porosity, the water absorption and loss rate in hot water were determined.
    RESULTS AND CONCLUSION: It was more suitable to meet the requirements when the mixed ratio of SF to CS was 1 to 1. The pore size was 90-280 μm and the average pore size was 151.72 μm. The porosity was (92.72±4.78)%. The water absorption was (92.72±4.78)%. The loss rate in hot water after crosslinking was decreased compared before crosslinking, and there was a significant difference (P < 0.05). The mixed ratio of SF to CS is at 1 to 1 meeting the physicochemical requirements of cartilage tissue engineering scaffolds. The SF/CS composite material is expected to be an ideal scaffold material for cartilage tissue engineering.

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    Prevention and treatment of post-traumatic orbital tissue adhesion by using chitosan membrane
    Ren Ming-yu, Zhao Hong, Li Jing
    2011, 15 (47):  8825-8828.  doi: 10.3969/j.issn.1673-8225.2011.47.021
    Abstract ( 383 )   PDF (1523KB) ( 341 )   Save

    BACKGROUND: The macromolecular biomaterial, chitosan membrane (CM) with low immunogenicity, can be resolved and generally used in the field of soft tissue adhesion. But the orbit included much subtle texture and owned the minor space, the application of CM in the orbit has not been general at present.
    OBJECTIVE: To observe the influence and mechanism of CM in prevention and treatment of soft tissue adhesion after orbital trauma.
    METHODS: A total of 10 rabbits with 20 orbits were randomly divided into two groups, namely, CM group and control group. The adhesion models of rabbits were established first, then the adhesion tissues were separated after two weeks, and CM about   15 mm×15 mm were implanted between the lesion and periost. The CM was not used in the control group.
    RESULTS AND CONCLUSION: Four weeks after experiment, the adhesion degree was lower in the CM group than the control group (P < 0.01). The quantity of fibroblasts in unit area and absorbance value in unit area of collagen fibers were both significantly lower in the CM group than the control group (P < 0.01). Relative area of collagen fibers was also lower in the CM group as compared with the control group (P < 0.01). The structures of retina and optic nerve were not changed in histopathology. CM can inhibit the proliferation of fibroblasts, and decrease the synthesis of collagen fibers, which is effective on preventing adhesion after the orbital trauma. And it has no effect to the retina and optic nerve.

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    Effect of cerium oxide and erbium oxide as colorants on the chromaticity and mechanical properties of dental zirconia ceramic
    Li De-chao, Liu Wen-bin, Zhao Shu-jin, Feng Hui-shan, Yang Dong-hong, Gao Shi-jun
    2011, 15 (47):  8829-8832.  doi: 10.3969/j.issn.1673-8225.2011.47.022
    Abstract ( 543 )   PDF (1223KB) ( 1029 )   Save

    BACKGROUND: Some studies have proposed adding rare earth oxides into zirconia, to change the color of zirconia oxide in order to meet the clinical requirements of the mouth for beautility.
    OBJECTIVE: To evaluate the chromatic value and mechanical properties of zirconia added with rare earth oxides.
    METHODS: ShadeEye computer colorimetric meter was used to test the L * a * b * values of the specimens. X-ray diffraction (D/Max-RB) was used to analyze the crystalline structure of materials. Using EZ-100 universal experiment machine, three-point bending strength was measured. The bending fracture microscopic form was observed under scanning electron microscope.
    RESULTS AND CONCLUSION: ①The main crystalline structure of sintered ziconia specimens was tetragonal zirconia, and there was no another crystalline structure. ②The mixture of cerium oxide and ziconia presented with pale yellow; the mixture of erbium oxide and ziconia presented with pale pink, and the color deepened with the dose increase. Two hybrid specimens made ziconia presenting orange, and with the color deepened with the dose increase. ③The flexural strength of the materials added with cerium dioxide or erbium oxide alone was in downtrend. The flexural strength of the specimens added with the mixture of cerium dioxide and erbium oxide was decreased with the increase of cerium dioxide and erbium oxide. However, the single cerium dioxide only led to a small decrease in the flexural strength, and erbium oxide significantly increased the decrease of flexural strength. The color of zironia mixed with rare earth oxides can be changed, and a certain flexural resistance appears.

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    Heparin immobilization on the surface of expanded polytetrafluoroethylene vascular grafts and its anticoagulation effect with replacement of canine inferior vena cava
    Zhang Ming, Liu Chang-jian, Liu Chen, Qiao Tong
    2011, 15 (47):  8833-8836.  doi: 10.3969/j.issn.1673-8225.2011.47.023
    Abstract ( 392 )   PDF (1253KB) ( 436 )   Save

    BACKGROUND: After various types of vein grafts are implanted in the body, due to the incompatibility of blood vessels with artificial surface and slow venous blood flow, low pressure, it is easy to lead to intravascular thrombosis
    OBJECTIVE: To study a new way to immobilize the heparin molecule on the expanded polytetrafluoroethylene (ePTFE) graft’s surface and to evaluate its anticoagulation effect by replacing the canine inferior vena cava.
    METHODS: Chitosan molecule with photoallergic radical was firstly immobilized to the surface of ePTFE graft, secondly heparin was immobilized to the chitosan molecule under acid condition. Thus the ePTFE graft’ surface was immobilized with a layer of heparin molecule. Local canines’ vena cava was replaced with control and experiment ePTFE grafts. Angiography from the femoral vein and scanning electron microscope (SEM) observation were performed.
    RESULTS AND CONCLUSION: All canines’ vena cava which was replaced with untreated ePTFE grafts was thrombosed since the first week after operation, while the canines’ vena cava which was replaced with ePTFE grafts immobilized with heparin kept unobstructed until the fourth week after operation. SEM observation found that mass of platelets, red blood cells and fibrin deposited on the untreated grafts’ surface, while seldom blood components could be observed on the grafts’ surface which were immobilized with heparin. ePTFE graft which surface is immobilized with heparin has an excellent anticoagulant ability, and it is an ideal venous graft in clinical usage.

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    Microdialysis in a conscious rabbit ocular posterior segment and the pharmacokinetics study of vancomycin after intravenous and intravitreal administration
    Wang Ya-ling, Yu Hai-tao, Chen Xiao-long
    2011, 15 (47):  8837-8841.  doi: 10.3969/j.issn.1673-8225.2011.47.024
    Abstract ( 392 )   PDF (1614KB) ( 555 )   Save

    BACKGROUND: Currently, in the pharmacokinetic studies of the eye, the sample selection is often carried out in vitro, and there are many disadvantages.
    OBJECTIVE: To develop a novel ocular microdialysis technology in conscious rabbit eyes, and to compare the difference of the pharmacokinetics of vancomycin after intravenous and intravitreal administration.
    METHODS: Fifteen healthy and mature rabbits were used to prepare endophthalmitis models and were randomly divided into three groups (intravenous administration, intravitreal administration and intravenous+intravitreal administration) at 24 hours after microdialysis probe was implanted. Vitreous vancomycin concentration was detected by high performance liquid chromatography-ultraviolet (HPLC-UV).
    RESULTS AND CONCLUSION: The vancomycin concentration in the intravenous administration group was much lower than the other two groups. At 72 hours after administration, the vancomycin concentrations in the intravitreal administration and intravenous+intravitreal administration groups reached a therapeutic concentration. It is indicated that microdialysis combined with HPLC-UV can continuously and in vivo detect the vancomycin concentration in the vitreous body, and single administration of vancomycin cannot reach a therapeutic concentration.

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    Chronic biocompatibility of chitosan/poly(lactic-co-glycolic acid) tissue engineered nerve transplanted into Beagles
    Zhu Qi, Yang Yu-min, Wu Jian, Hu Wen, Gu Xiao-song
    2011, 15 (47):  8842-8846.  doi: 10.3969/j.issn.1673-8225.2011.47.025
    Abstract ( 281 )   PDF (1500KB) ( 492 )   Save

    BACKGROUND: Short-term biocompatibility test within 2 months has showed that chitosan, polylactic acid and polyglycolic acid have no toxicity in rat peripheral nerve, which can be used as tissue engineered nerve materials.
    OBJECTIVE: To evaluate the chronic biocompatibility of the chitosan/poly(lactic-co-glycolic acid) (PLGA) tissue engineered nerve transplanted into beagles.
    METHODS: The tissue engineered nerve was prepared through inserting the PLGA filaments into chitosan conduits, and then transplanted into beagles bridging sciatic nerve 50 mm defect, and autologous nerve graft was taken as control at the same time.
    RESULTS AND CONCLUSION: The status, appetite and activity of beagles which were transplanted with chitosan/PLGA tissue engineered nerve were good and the weight gain were similar to the autologous nerve graft group. Hematology and serum biochemical tests results after 2, 4, 6 months were also similar to the control group and there were no significant difference between two groups. Necrosis and degeneration was not found in the regenerated nerve as well as the surrounding tissues. And the tissue biopsies of the heart, liver, spleen, lung, kidney and other major organs were not abnormal. The chronic biocompatibility of the chitosan/PLGA tissue engineered nerve which was transplanted into Beagles for 6 months was good.

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    Determination of entrapment efficiency of albendazole chitosan nanoparticles
    Lian Wen-yuan, He Cheng-hui, Xing Jian-guo, Wang Xin-chun, Huang Chuan-sheng
    2011, 15 (47):  8847-8850.  doi: 10.3969/j.issn.1673-8225.2011.47.026
    Abstract ( 362 )   PDF (1272KB) ( 392 )   Save

    BACKGROUND: In the course of studying nanoparticles, the entrapment efficiency is a very important index of nanoparticle quality evaluation.
    OBJECTIVE: To establish a method for determination of the entrapment efficiency of albendazole chitosan nanoparticles.
    METHODS: Reverse dialysis method was used to determine the entrapment efficiency of albendazole chitosan nanoparticles by analyzing the amount of albendazole with ultraviolet spectrophotometry.
    RESULTS AND CONCLUSION: The reverse dialysis method could separate albendazole chitosan nanoparticles from free albendazole, its equilibrium time and the dialyzed volume ratio was 9 hours and 1:15, respectively. The sample recovery rate was (99.59±0.36)%. The average entrapment efficiency of albendazole chitosan nanoparticles was (51.73±0.18)%. Reverse dialysis method was convenient and accurate. It was suitable for determining the entrapment efficiency of albendazole chitosan nanoparticles.

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    Effect of surface properties of modified hydroxyapatite on the adherent growth of osteoblasts
    Wei Jun-chao, Chen Lei, Chen Yi-wang, Chen Xue-si
    2011, 15 (47):  8851-8854.  doi: 10.3969/j.issn.1673-8225.2011.47.027
    Abstract ( 302 )   PDF (1290KB) ( 566 )   Save

    BACKGROUND: The alteration of biomaterial surface properties may affect interaction between the biomaterials and cells, which will elicit diverse cell behaviors, such as cell adhesion, gene expression and so on.
    OBJECTIVE: To investigate the effects of surface properties of a new type of polymer modified hydroxyapatite (HA) and to determine its effects on adhesion ability of osteoblasts.
    METHODS: HA and modified HA were prepared according to author’s previous experiments. The zeta potential of modified HA was measured by using zeta potential analyzer. The water contact angle and cell attachment test were carried out to investigate the effect of surface modification on the cell adhesion ability and the biocompatibility of modified HA.
    RESULTS AND CONCLUSION: The surface of modified HA was hydrophobic, and its water contact angle was 93°. Cell attachment test showed that the number of osteoblasts attached on the modified HA surface was higher than that on the HA surface. It is indicated that the surface modification enhances the biocompatibility of HA.

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    Comparison of the fracture resistance of different fiber posts in combination with auxiliary piles in restoration of flared roots
    Zhang Qin, Yu Hai-tao
    2011, 15 (47):  8855-8858.  doi: 10.3969/j.issn.1673-8225.2011.47.028
    Abstract ( 295 )   PDF (1267KB) ( 597 )   Save

    BACKGROUND: In recent years, the clinical use of glass fiber and carbon fiber post has obtained a more satisfactory result, but the bonding between the fiber post and root canal dentin also need further research.
    OBJECTIVE: To compare the fracture resistance ability of three post-core systems in the repair of the first premolar, and to analyze the influential factors.
    METHODS: Totally 36 single rooted first premolars from orthodontic patients were divided into A, B and C three groups, respectively after endodontic treatment (group A: MACRO-LOCK quartz fiber with FIBERCONETM post-core system, group B: Nr-Cr post-core system; group C: MACRO-LOCK quartz fiber post-core system). After restoration with Nr-Cr PFM full crown, they were fixed in universal testing machine for loading, until the specimen destroyed instantly, to record the load to fracture (in Newton) and the load-displacement curve.
    RESULTS AND CONCLUSION: The median fracture values of three groups were as follows: Group A: (403.99±15.91) N, Group B: (304.31±16.95) N, Group C: (233.48±22.56) N. The group A showed a significantly higher level of fracture strength compared to the other groups and the fracture model of MACRO-LOCK quartz fiber with FIBERCONETM post-core system allowed the possibility of re-preparation (P < 0.05). The MACRO-LOCK quartz fiber with FIBERCONETM post-core system showed a significantly higher level of fracture strength compared to the other groups and strengthened the flared roots.

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    Effect of organic modifiers on the size of hydroxyapatite prepared by hydrothermal method
    Wu Zhan-ao, Xuan Zhi-gang, Wang Chun-feng, Liu Si, Sun Min
    2011, 15 (47):  8859-8862.  doi: 10.3969/j.issn.1673-8225.2011.47.029
    Abstract ( 359 )   PDF (980KB) ( 389 )   Save

    BACKGROUND: Nanosized hydroxyapatite with different size and morphology has different surface performance and bioactivity. Different sized nanohydroxyapatite was synthesized via surface modification of hydroxyapatite; by this method, the application of nanosized hydroxyapatite can be extended.
    OBJECTIVE: To analyze the effect of experimental parameters, such as type of organic modifiers and pH value, etc. on the size of hydroxyapatite synthesized by hydrothermal method.
    METHODS: Hydroxyapatite with different sizes was synthesized starting from phosphoric acid and calcium nitrate and using D-sorbitol, sodium polyacrylate and Tween 80 as organic modifiers at different pH values.
    RESULTS AND CONCLUSION: In the absence of organic modifier, the size of hydroxyzpatite was decreased rapidly with the increase in pH values. The presence of sodium polyacrylate was beneficial to the formation of small-size hydroxapatite. The addition of D-sorbitol favored the formation of large-size hydroxyapatite. The presence of Tween 80 decreased the size of hydroxyapatite in some extent.

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    Two inlay processing techniques effects on the mechanical function of resin inlays
    Liu Cheng-guang, Deng Jing, Yuan Chang-qing
    2011, 15 (47):  8863-8865.  doi: 10.3969/j.issn.1673-8225.2011.47.030
    Abstract ( 314 )   PDF (326KB) ( 488 )   Save

    BACKGROUND: Composite resin functions as a practical resin restoration material with beautiful outlook, modifying its mechanical properties has become a hot spot in research.
    OBJECTIVE: To prepare resin specimens with two kinds of inlay curing machines: CERAMAGE and TESCERA, and to compare the mechanical properties of these specimens.
    METHODS: The resin specimens supporting two machines were cross-matched with these machines and then divided into four groups: Group A was Tescrea resin prepared with TESCERA machine; group B was Tescrea resin prepared with CERAMAGE machine; group C was Ceramage resin prepared with CERAMAGE machine; group D was Ceramage resin prepared with TESCERA machine. The standard specimens were determined for compressive strength, hardness and flexural strength. 
    RESULTS AND CONCLUSION: The compressive strength and hardness in group A were higher than those in other three groups, and group B exhibited higher compressive strength and hardness than groups C and D (P < 0.05). The flexural strength in groups C and D was higher than that in groups A and B (P < 0.05), there was no significant difference between groups C and D, neither betweens group A and B. The experimental findings indicate that TESCERA inlay machine and Tescera resin achieve the optimal mechanical properties.

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    Application of gelfoam in embolization for treatment of hemoptysis in pulmonary tuberculosis patients
    Li Lin-zhong, Li Na
    2011, 15 (47):  8866-8868.  doi: 10.3969/j.issn.1673-8225.2011.47.031
    Abstract ( 357 )   PDF (1114KB) ( 371 )   Save

    BACKGROUND: Current materials used for bronchial artery embolization are gelatin sponge, PVA particles, alginate beads, coils, etc.
    OBJECTIVE: To summarize the effect of gelfoam on bronchial artery embolization for treatment of hemoptysis.
    METHODS: A computer based search of CNKI and VIP databases was performed to retrieve relevant articles published 1995-01/2011-05. The keywords were “hemoptysis, embolization, tuberculosis, gelfoam” in Chinese and English. Repetitive articles or Meta analysis were excluded.
    RESULTS AND CONCLUSION: Totally 17 articles were included in result analysis, which reviewed the application of gelfoam in bronchial artery embolization for treatment of hemoptysis, including the introduction of gelfoam embolization agents, arterial embolization techniques, and curative effect and deficiency of gelfoam in the treatment of hemoptysis through bronchial artery embolization. Gelfoam with high-pressure and high-temperature treatment is satisfactory and very economical for treatment of hemoptysis, which is preferred in China.

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    Effectiveness of prophylactic antibiotic administration for inguinal hernia repair with biological patch: A Meta-analysis
    Liang Zhao-ke, Sun Lin
    2011, 15 (47):  8869-8872.  doi: 10.3969/j.issn.1673-8225.2011.47.032
    Abstract ( 301 )   PDF (1212KB) ( 429 )   Save

    BACKGROUND: Prophylactic antibiotics are very common following tension-free inguinal hernia repair; however, indiscriminate use of antibiotics will increase the cost and antibiotic resistance of patients.
    OBJECTIVE: To systematic review the effectiveness of prophylactic antibiotic administration in reducing postoperative wound infection rates in inguinal hernia repair.
    METHODS: We searched PubMed, EMBASE, the Cochrane Library, CBM, CNKI, VIP databases, and the data were analyzed using Review Manager 5.0.
    RESULTS AND CONCLUSION: Eight randomized controlled trials were included. The Meta analysis showed that there was significant difference between prophylactic antibiotic group and placebo group in surgical site infection rate [RR=0.68, 95%CI(0.49, 0.94)]; The heterogeneity analysis did not reach statistical significance neither for the sensitivity analysis [RR=0.78, 95%CI(0.56, 1.10)] nor in the sub-groups analysis[RR=0.63, 95%CI(0.16, 2.57) for comparison between β-lactam antibiotics and placebo] [RR=0.66, 95%CI(0.41, 1.06) for comparison between cephalosporins and placebo]. There was not enough evidence to support routine prophylactic antibiotic administration for inguinal hernia repair.

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    Effect of gold alloy restorations and nickel-chromium restorations to the periodontal tissue of the Chinese: A systematic review
    Li Jing-wen, Li Chun-jie, Lü Jun, Liu Wen-wen, Liang xing
    2011, 15 (47):  8873-8876.  doi: 10.3969/j.issn.1673-8225.2011.47.033
    Abstract ( 407 )   PDF (1592KB) ( 409 )   Save

    BACKGROUND: Chinese researchers have done many corresponding clinical trials and mostly agree that, compared with nickel-chromium restorations, gold alloy restorations are better to the Chinese periodontal tissue in the long term. However, there is still no relevant systematic review analyzing and evaluating those results in a more rigorous and convincing way.
    OBJECTIVE: To assess the effect of nickel-chromium restorations and gold alloy restorations to the periodontal tissue of the Chinese.
    METHODS: Electronic searches were conducted in China Biology Medicine disc (CBMdisc, 1978-01/2011-03) and China National Knowledge Infrastructure (CNKI, 1979-01/2011-03). Hand-searching covered 10 Chinese dental journals. Randomized controlled trials satisfying the eligible criteria were selected and the risks of bias were assessed. With the data extracted by two well-trained investigators independently, Meta-analysis was processed by Revman 5.0.
    RESULTS AND CONCLUSION: Six studies with moderate risk of bias were included. The results indicated gold alloy restorations could reduce 79% of the risk of post-restorative gingivitis in nickel-chromium restorations (P < 0.05), and gold alloy restorations could also reduce the risk of gingival coloration by 94% of nickel-chromium restorations (P < 0.05). The results on the restoration fitness with four studies pooled indicated better restoration fitness in gold alloy restorations than nickel-chromium restorations (P < 0.05). Present evidences indicated that gold alloy restorations have lower risk in inducing gingivitis and gingival coloration compared to nickel-chromium. But more high quality studies are still needed to confirm the conclusion.

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    Research advances of chitosan nanoparticles as genetic carriers
    Li Guang-feng, Yang Jian-dong
    2011, 15 (47):  8879-8882.  doi: 10.3969/j.issn.1673-8225.2011.47.035
    Abstract ( 391 )   PDF (1499KB) ( 834 )   Save

    BACKGROUND: In recent years, studies of chitosan as a genetic carrier multiplied for its own characteristics have been increased gradually.
    OBJECTIVE: To review the research advances of chitosan nanoparticles as a genetic carrier, and to further improve the effect of gene therapy.
    METHODS: Web of Science and CNKI databases were searched (2000-01/2011-04) with the key words of “chitosan, gene” in English and Chinese, respectively. 120 articles related to gene carrier and chitosan were selected firstly, and finally 31 articles were included in result analysis.
    RESULTS AND CONCLUSION: Chitosan gene nanoparticle as the non viral gene carrier will play a vital role in this field in the future. Research of chitosan genetically modified system will be more deeply. How to mark, visually track the process of chitosan DNA complex into different cells, make the genetic carrying mechanism clear, improve efficiency, make gene therapy into clinical application as soon as possible will be the main research points in the future.

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    Preparation and application of medical chitosan membranes
    Liu Gui-chun, Wang Ling-feng, Ba Te
    2011, 15 (47):  8883-8886.  doi: 10.3969/j.issn.1673-8225.2011.47.036
    Abstract ( 364 )   PDF (1489KB) ( 517 )   Save

    BACKGROUND: Chitosan membranes in artificial kidney-dialysis membranes, artificial skin, oral-membranes ulcers, periodontal guided tissue regeneration membranes, controlled-release drug delivery and other film-related tissue engineering have made gratifying achievements.
    OBJECTIVE: To summarize the preparation of chitosan membranes and the current application for local drug delivery.
    METHODS: A computer online search was performed to find papers published between 1980 and 2011 in databases of CNKI and Duxiu academic search. Documents concerning the reviews and experimental studies about the preparation of chitosan membranes used in drug delivery carrier were reviewed.
    RESULTS AND CONCLUSION: A total of 22 literatures were included in this study. Preparation condition of chitosan membranes is closely related with their application in drug delivery as carrier, but the summary specification and its relevance is not yet clear, still looking forward to a lot of research. Preparation conditions of chitosan membrane used in drug delivery still need further research to explore the suitable condition to prepare chitosan membranes as optimized drug delivery carriers, which is the focus of researchers.

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    Application and progress in the materials for drug controlled release
    Wang Hong-xin, Chen Xiao-ming
    2011, 15 (47):  8887-8890.  doi: 10.3969/j.issn.1673-8225.2011.47.037
    Abstract ( 402 )   PDF (1730KB) ( 1031 )   Save

    BACKGROUND: Polymer materials play an important role in drug controlled release system. But nano-inorganic materials are studied as a new drug and gene controlled delivery carrier and appears to be very promising, so more considered are paied attention to the design and research of drug controlled release carrier materials.
    OBJECTIVE: To summarize the applications and research progress of drug controlled release materials.
    METHODS: Database of CNKI and Elsevier SD (1999-01/2011-01) were retrieved to search the related articles about the materials for drug controlled release using the keywords of “polymer, mesoporous materials, inorganic silicon, calcium phosphate, controlled release” in Chinese and English. Finally, 25 articles were included in review analysis.
    RESULTS AND CONCLUSION: Drug controlled release carrier materials are widely drawn by their low dosage, long duration and good target effect. But there are still some deficiencies such as the drug inactivation and loss of biological activity after drug loading. The clinical application of drug controlled release carrier materials will be used more widely along with the development of composite carriers for drug delivery and transdermal drug delivery systems.

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    Selection and application of oral all-ceramic crown materials
    Cheng Jin-chun
    2011, 15 (47):  8891-8894.  doi: 10.3969/j.issn.1673-8225.2011.47.038
    Abstract ( 414 )   PDF (1545KB) ( 856 )   Save

    BACKGROUND: Compared with porcelain fused to metal crown, the all-ceramic crown materials have been more and more applied in clinical anterior teeth restoration due to natural and stable color, low thermal conductivity, non-electric conductivity, wear resistance, good biocompatibility, non-transparent metallic, and relatively simple operations.
    OBJECTIVE: To summarize the characteristics, production methods and clinical application of different all-ceramic crown materials.
    METHODS: A computer-based online retrieval was performed in Wanfang database and Medline database between 2001 and 2011 for articles regarding the characteristics, production methods, physical and mechanical strength, chemical and biological compatibility, aesthetic appearance and clinical application of different all-ceramic crown materials.
    RESULTS AND CONCLUSION: Currently, the clinically used all-ceramic materials can be divided into slip casting porcelain, casting ceramic, heat-pressed glass ceramic, glass-infiltrated ceramics, densely-sintered aluminum oxide polycrystalline, partially stabilized tetragonal zirconium oxide polycrystalline, and nano-composite ceramic. Different materials have its specific mechanical properties and clinical indications, so clinical selection must be based on specific conditions. With the advance of high-strength ceramic studies, all-ceramic restorations become widely applied, and high-aluminum ceramic represented by In-ceram and zirconia all-ceramic crowns can achieve good effects within a short and long term, foreign clinical experiments have conducted the enlargement from anterior crown to rear crown and bridge. To insure optimum long-term clinical results of all-ceramic crown restorations, the key is to overcome the fragmentation of dental prosthesis, mainly through modifying the composition, improving the anti-smash property of ceramic materials, and a series of prevention measures.

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    Role of biological materials in preventing postsurgical adhesion and promoting tendon healing after sports injury
    Zhang Hai-ling
    2011, 15 (47):  8895-8898.  doi: 10.3969/j.issn.1673-8225.2011.47.039
    Abstract ( 358 )   PDF (1461KB) ( 512 )   Save

    BACKGROUND: Tendon adhesion following sports injury is a big problem puzzling athletes for a long time, which affects training and sport technique. Its prevention has been a medical issue of common concern.
    OBJECTIVE: To review the effect of biological materials on preventing postsurgical adhesion and promoting tendon healing following sports injury.
    METHODS: Using “biological material, tendon injury, prevent antistickm repair” in Chinese and English as the key words, a computer search was performed for articles published between January 1980 and December 2010. Articles related to the biological materials and tissue engineering femoral defects were included; repeated study or Meta analysis articles were excluded.
    RESULTS AND CONCLUSION: Tendon healing following injury includes two forms, endogenous healing and exogenous healing. The ideal way to prevent tendon adhesion is to improve the nutritional state and the surrounding environment of the tendon so as to promote endogenous healing and restrain/ease exogenous healing. This paper explores the action mechanism and characteristics of several biomaterials in order to better play the role of biomaterials in prevention of tendon adhesion.

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    Biomedical materials for adhesion repair after tendon injury
    Jin Dong, Zhang Guo-zhong
    2011, 15 (47):  8899-8902.  doi: 10.3969/j.issn.1673-8225.2011.47.040
    Abstract ( 323 )   PDF (1814KB) ( 372 )   Save

    BACKGROUND: Selection of biomaterials against tendon adhesion is a hotspot in the medical field.
    OBJECTIVE: To summarize the clinical application of biomaterials in adhesion repair following tendon injury.
    METHODS: A computer-based search of VIP database (1999-01/2009-10) was performed for articles about biomaterials for postoperative adhesions after tendon injury. Totally 30 articles were included.
    RESULTS AND CONCLUSION: Chitosan has good biological properties, which is a non-toxic, non-irritating, non-antigenic, and biodegradable biomaterial with good tissue compatibility, and it has a significant role in the prevention of tendon adhesion. Biomedical fibrin glue has a fine hematischesis and sealing function, good biocompatibility, favorable biodegradation function and a certain sterilization, which can reduce and control traumatic inflammation reactions. Numerous clinical studies and animal studies have shown that medical sodium hyaluronate, gelatin sponge, and chymotrypsin have a preventive role in postoperative tissue adhesion.

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    Materials for repairing calvarial defects: A 20-year bibliometric analysis based on Web of Science
    Xu Zai-hua, Tang Tao, Pan Dong-sheng, Fan Di, Song Zhen-quan, Xue Hong-li
    2011, 15 (47):  8903-8906.  doi: 10.3969/j.issn.1673-8225.2011.47.041
    Abstract ( 287 )   PDF (913KB) ( 401 )   Save

    BACKGROUND: Materials for repairing calvarial defects is a hot topic both in China and abroad, however, bibliometric analysis addressing materials for repairing calvarial defects is insufficient.
    OBJECTIVE: To summarize the current status and frontier of studies addressing materials for repairing calvarial defects via analyzing relative literatures with the method of bibliometric analysis.
    METHODS: Literatures addressing the materials for repairing calvarial defects in the Web of Science from 1990 to 2010 were analyzed with the method of bibliometrics. It mainly discusses materials for repairing calvarial defects in the aspects of distribution of publication years, countries, institutions, journals, citations, as well as science categories.
    RESULTS AND CONCLUSION: From 1990 to 2010, 174 literatures on the materials for repairing calvarial defects were added to SCI. Literature is annual increased, and the United States ranks the first with 58 papers, including 8 core institutes, 2 core journals and 4 classical references. From the analysis of research dynamic and development trend, we put forward some proposes for China's in-depth research on materials for repairing calvarial defects.

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    Relationship of biomaterials related infections and transforming growth factor levels in the peripheral blood of lung cancer patients
    Lei Yu-jie, Huang Yun-chao, Yang Li, Guo Feng-li, Zhao Guang-qiang, Xu Yu-shan, Chen Xiao-bo, Yang Kai-yun
    2011, 15 (47):  8907-8910.  doi: 10.3969/j.issn.1673-8225.2011.47.042
    Abstract ( 245 )   PDF (421KB) ( 407 )   Save

    BACKGROUND: Permanent or transient implantation of biomaterials can result in biomaterials-centered infections (BCI) in lung cancer patients.
    OBJECTIVE: To investigate the relationship between BCI and peripheral blood transforming growth factor β1 (TGF-β1) in patients with lung cancer.
    METHODS: A total of 248 lung cancer patients undergoing in vivo intravascular catheter indwelling > 7 days were included. Quantitative method was used for intubation, bacteriological culture and paired blood culture, and API Staph strips were adopted for positive patients. While enzyme-linked immunosorbent assay was used to detect TGF-β1 levels in the peripheral blood of patients with lung cancer and 75 healthy volunteers as normal controls.
    RESULTS AND CONCLUSION: Among the 248 patients, there were 82 BCI-positive cases, and 166 BCI-negative cases. Thirteen patients were confirmed to have catheter-related bloodstream infection. There were 48 Gram-positive bacteria, 24 Gram-negative bacilli, and 10 fungal. The levels of TGF-β1 were higher in BCI-positive patients than BCI-negative patients (P < 0.05); the levels of TGF-β1 in the BCI-negative group were higher than those in the normal control group (P < 0.05). For lung cancer patients with nosocomial infection induced BCI, there are various species of pathogenic bacteria, and Gram-positive bacteria are more common. To detect TGF-β1 levels in patients with lung cancer is of significance for early prevention of BCI.

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    Postural reduction and percutaneous vertebroplasty for severe osteoporotic vertebral compression fractures
    Chu Ge, Ka Ha-er•Ai Ken-mu
    2011, 15 (47):  8911-8914.  doi: 10.3969/j.issn.1673-8225.2011.47.043
    Abstract ( 376 )   PDF (1151KB) ( 339 )   Save

    BACKGROUND: In the severely collapsed vertebrae of more than two-thirds of their original height, vertebroplasty is regarded as a contraindication.
    OBJECTIVE: To verify percutaneous vertebroplasty combined with postural reduction can treat severe osteoporotic vertebral compression fractures.
    METHODS: Seventy-five patients with single-level vertebral compression fracture were treated with postural reduction and vertebroplasty. They were divided into three groups according to the onset duration, < 4 weeks group, 4-8 weeks group and >   8 weeks group. In 30 of 75 patients, the vertebral body was severely collapsed more than two-thirds of its original height. Compression ratio (anterior height/posterior height) was calculated, the Cobb angle was measured and the degree of re-expansion was analyzed according to the onset duration.
    RESULTS AND CONCLUSION: The mean compression ratio was 0.60±0.15 initially and increased to 0.75±0.17 after vertebroplasty. The mean Cobb angle was (16.14±11.29)° before treatment and corrected to (10.71±12.08)° after vertebroplasty. Over the onset duration, the compression ratio and Cobb angle decreased. Twenty-eight of thirty (93%) severely collapsed vertebrae re-expanded after postural reduction, which made vertebroplasty possible. Patients’ pain relieved after vertebroplasty. It is indicated that the onset duration of vertebral fractures is shorter, and the degree of re-expansion, correction of Cobb angle, improvement of spinal sagittal kyphosis and pain relieve after vertebroplasty degree are higher.

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    Ceramic to metal combination properties of self-made Co-Cr alloy
    Zhao Xiang-yu, Zhan De-song, Sun Yi, Fan Zhe
    2011, 15 (47):  8915-8918.  doi: 10.3969/j.issn.1673-8225.2011.47.044
    Abstract ( 358 )   PDF (1204KB) ( 434 )   Save

    BACKGROUND: Co-Cr alloy has better biocompatibility, anti-corrosion and stability than Ni-Cr and Ti alloy. It is more and more used extensively.
    OBJECTIVE: Compared to the Germany BEGO cobalt-chromium alloy, to make initial evaluation to the combination of ceramic to metal of the new-type Co-Cr alloys.
    METHODS: VITA MK95 porcelain powder of 8 mm×3 mm×1 mm was fused to the two types of alloys of 25 mm×3 mm×0.5 mm and the combination of ceramic to metal of the two types of alloys was tested using three-point flexure bond test and scanning electron microscope.
    RESULTS AND CONCLUSION: For two kinds of alloys after strength loading, ceramic layer cracked and detached completely at the same time, gray-black oxide film formed on the alloy surface, and there was no significant residual porcelain (P > 0.05). The bonding of ceramic to metal of the new type Co-Cr alloys is greater than the basic value of the required ISO (25 MPa), which do not different from that of the Germany BEGO and meet the clinical requirements.

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    Acellular dermal matrix patch for treatment of pediatric anal fistula
    Wang Li-ya, Dong Yan-qing, Zhang Peng-ju, Gong Ying-xin, Liu Feng, Niu Hui-zhong, Liu Chun-yi
    2011, 15 (47):  8919-8922.  doi: 10.3969/j.issn.1673-8225.2011.47.045
    Abstract ( 368 )   PDF (1719KB) ( 487 )   Save

    BACKGROUND: Allogenous acellular dermal matrix patches have a good clinical effect on adult anal fistula.
    OBJECTIVE: To investigate the feasibility and approach of allogenous acellular dermal matrix patches in repair of pediatric anal fistula.
    METHODS: Totally 138 cases of pediatric anal fistula were randomized into two groups to receive allogenous acellular dermal matrix patch suture: group A, double-layer suture closure; group B, non-free suture.
    RESULTS AND CONCLUSION: All the cases were followed for 6 months. There were no perianal swelling, abnormal appearance, induration, anal stenosis and anal shape change. After repair, some children presented with reuben from the fistula that was healed without treatment. The number of children with reuben from the fistula in group A was higher than that of group B (P < 0.01), and the appearance time was also earlier in group A (P < 0.05). Allogenous acellular dermal matrix patch has a good effect on pediatric anal fistula.

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    Application of calcium sulfate artificial bone in 34 cases of tibial plateau fractures
    Hu Hai-bo, Li Ping-sheng, Gong Jun
    2011, 15 (47):  8923-8926.  doi: 10.3969/j.issn.1673-8225.2011.47.046
    Abstract ( 378 )   PDF (1658KB) ( 610 )   Save

    BACKGROUND: The current treatment of tibial plateau fractures is bone graft repair of bone defects after reduction surgery, but the selection of filling materials for bone defects is controversial.
    OBJECTIVE: To evaluate the clinical effect of injectable artificial bone combined with plate fixation for treatment of tibial plateau fractures.
    METHODS: From January 2009 to December 2010, 34 cases of collapse tibial plateau fractures were treated by injectable calcium sulfate bone graft combined with supportive plate reconstruction. All patients preoperatively underwent radiography, CT scanning and three-dimensional reconstruction in order to accurately understand the extent of fracture and fracture collapse and the flip direction of shattered fragments.
    RESULTS AND CONCLUSION: All patients were followed-up for 6 months to 2.5 years. The X-ray films showed anatomic reduction or near anatomic reduction, but nonunion or infection was not found. Wound exudations were observed in two cases and the bacterial culture was negative. According to evaluation of Lysholm score, the results were excellent in 13 cases, good in 17 cases, and fair in 4 cases. The Lysholm scoring of knee function showed that the mean score was (88.3±5.1) points. Minimally invasive injectable calcium sulfate bone graft combined with plate fixation for collapse tibial plateau fracture can effectively prevent the further loss after reduction, improve the long-term results. Minimally invasive injectable calcium sulfate as an artificial bone substitute materials has good prospects for clinical application.

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    Application of acellular dermal matrix in treatment of unilateral vocal cord paralysis
    Liu Rong-rong, Li Hong-tao, Zhang Min, Xiao Ya
    2011, 15 (47):  8927-8930.  doi: 10.3969/j.issn.1673-8225.2011.47.047
    Abstract ( 405 )   PDF (1452KB) ( 541 )   Save

    BACKGROUND: Acellular dermal matrix (ADM), as a new material which has good biologic and structural compatibility, is generally applied to tissue repair and padding in operations on head and neck.
    OBJECTIVE: To investigate the short- and long-term effect of ADM on vocal cord paralysis.
    METHODS: Totally 20 patients with unilateral complete recurrent laryngeal nerve paralysis were selected (the affected vocal cord was fixed at the side-centric position). The curative effect and risk of the operation of padding ADM under the affected vocal cord were observed by windowing on the lamina cartilaginis thyroidea under local anesthesia to improve trachyphonia.
    RESULTS AND CONCLUSION: Through the subjective assessment by patients and analysis of vocal acoustic parameters at perioperative stage, the curative effect was satisfactory. No patients suffered from special risk and adverse reaction. ADM with perfect histocompatibility and easy operability can be applied as the new padding material to migrate the paralytic vocal cord inward.

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