Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (47): 8745-8748.doi: 10.3969/j.issn.1673-8225.2011.47.002

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Antibacterial property and biocompatibility of digital silver loaded hydroxyapatite as artificial bone

Zhu Hui1,2, Yin Qing-shui2, Zhang Yu2, Zhang Yu2, Zhao Hua-fu2, Zhou Ye2   

  1. 1Graduate School of Southern Medical University, Guangzhou  510515, Guangdong Province, China
    2Department of Orthopedics, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou  510010, Guangdong Province, China
  • Received:2011-08-17 Revised:2011-09-30 Online:2011-11-19 Published:2011-11-19
  • Contact: Yin Qing-shui, Doctoral supervisor, Professor, Department of Orthopedics, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China gz_yqs@126.com
  • About author:Zhu Hui☆, Studying for doctorate, Physician, Graduate School of Southern Medical University, Guangzhou 510515, Guangdong Province, China; Department of Orthopedics, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China zhuhui04@126.com
  • Supported by:

    Military Research Project of Science and Technology during the Eleventh Five-Year Plan Period, Second Group Topic Special Purpose, No. 08Z010*

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Abstract:

BACKGROUND: The silver loaded coral hydroxyapatite (CHA-Ag) has received increasing attention as a new type of antibacterial bone graft material. Implant materials need well biocompatibility with human body.
OBJECTIVE: To observe the antibacterial property and biocompatibility of digital CHA-Ag as artificial bone.
METHODS: The coral hydroxyapatite powder was immersed in silver nitrate solution (AgNO3) with different concentrations in order to prepare CHA-Ag containing different contents of Ag+. Then the prepared CHA-Ag was mixed with poly lactic acid and burned by selective laser to prepare digital artificial bone material containing silver with special shape rapidly.
RESULTS AND CONCLUSION: The continuum source atomic absorption spectrometer showed that the Ag+ contents of CHA-Ag prepared with 10-2, 10-3, 10-4 and 10-5 mol/L AgNO3 were 2.31×10-1%, 3.18×10-2%, 6.75×10-3%, and 6.05×10-4% respectively. The inhibition ring test demonstrated that the inhibition ring diameter of CHA-Ag by 10-2 mol/L AgNO3 against staphylococcus aureus and Escherichia coli were (13.00±1.52) mm and (12.30 ±1.65) mm respectively; while that of 10-3 mol/L were (11.50±0.73) mm and (11.00±0.4) mm respectively, and no inhibition ring occurred in that of 10-4 and 10-5 mol/L AgNO3. Cytotoxicity test manifested that the cytotoxicity grades were 3, 1, 0 and 0 respectively according to the CHA-Ag prepared with 10-2, 10-3, 10-4 and 10-5 mol/L AgNO3. General toxicity test indicated that the artificial bone leaching liquor with 10-3 mol/L AgNO3 had no obvious acute cytotoxicity in mice. The CHA-Ag prepared with 10-3 mol/L AgNO3 has good biocompatibility and biological security, can inhibit the growth of staphylococcus aureus and Escherichia coli in vitro.

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