Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (47): 8787-8791.doi: 10.3969/j.issn.1673-8225.2011.47.012

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Histological study of enhancing the healing of anterior cruciate ligament autogenous graft with gene activated matrix materials

Qin Jian, Hou Zhi-qi, Wang Xin-liang, Liu Yong-yi, Gao Xing-hua, Lin Yu-jin, Huang Bao-hua   

  1. Department of Orthopaedics, the First People’s Hospital of Guangzhou, ?Guangzhou Medical University, Guangzhou  510180, Guangdong Province, China
  • Received:2011-04-03 Revised:2011-05-02 Online:2011-11-19 Published:2011-11-19
  • Contact: Hou Zhi-qi, Doctor, Professor, Department of Orthopaedics, the First People’s Hospital of Guangzhou, ?Guangzhou Medical University, Guangzhou 510180, Guangdong Province, China smilehou@yahoo.com.cn
  • About author:Qin Jian☆, Doctor, Associate chief physician, Department of Orthopaedics, the First People’s Hospital of Guangzhou, ?Guangzhou Medical University, Guangzhou 510180, Guangdong Province, China qinjian7537@126.com
  • Supported by:

    the Medical Health Research Program of Guangzhou City, No. 2009-ZDi-20*; the Science and Technology Plan Program of Guangdong Province, No. 2007B03100100*

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Abstract:

BACKGROUND: The basic concept of gene activated matrix (GAM) is a complex of biological materials and DNA plasmids to form a local gene delivery system.
OBJECTIVE: To learn the histological changes of rabbit anterior cruciate ligament reconstruction model treated with GAM materials, and to determine the difference in collagen express and miscrostrcture observation.
METHODS: Forty-eight New Zealand white rabbits were divided into two groups: experiment group and control group. The rabbit anterior cruciate ligament reconstruction model was established according to the previous method. In the experimental group, transforming growth factor β1 was locally injected in the bone tunnel but in the control group the wound was injected with blank plasmids.
RESULTS AND CONCLUSION: By light miscroscopy the number of fibroblasts in the control group was less than that in the experimental group, and the fibers arranged irregularly at different periods. In the experimental group, the number and appearance of fibroblasts were similar to normal strcture. There were more fibroblasts and collagens which became larger and arranged regularly. By electron miscroscopy we found the karyokinesis of cell miscrostructure was more active and some fibroblasts had a vibrant metabolism in the experimental group. There were plenty of endoplasm and mitochondria in cytoplast and more extracellularmatrix was filled in fibers. The findings indicate that transforming growth factor β1 can enhance the healing of reconstrctured ligament.

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