Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (53): 9986-9990.doi: 10.3969/j.issn.1673-8225.2011.53.026

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Effects of RelB shRNA lentivirus infection on immunological response of mouse bone marrow dendritic cells

Xie Jin-min1, 2, Bao Jie3, Dong Rui-qiang4, Yang Ming4, Wen Hao 1   

  1. 1Postdoctoral Research Station, First Affiliated Hospital of Xinjiang Medical University, Urumqi  830011, Xinjiang Uyghur Autonomous Region, China
    2Department of General Surgery, the 474 Hospital of Chinese PLA, Urumqi 830011, Xinjiang Uyghur Autonomous Region, China
    3Department of Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou  510515, Guangdong Province, China
    4Medical College of Shihezi Medical University, Shihezi 834407, Xinjiang Uyghur Autonomous Region, China
  • Received:2011-05-24 Revised:2011-08-21 Online:2011-12-31 Published:2011-12-31
  • Contact: Wen Hao, Doctor, Professor, Postdoctoral Research Station, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uyghur Autonomous Region, China Dr.wenhao@163.com
  • About author:Xie Jin-min☆, Doctor, Master’s supervisor, Associate chief physician, Postdoctoral Research Station, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uyghur Autonomous Region, China; Department of General Surgery, the 474 Hospital of Chinese PLA, Urumqi 830011, Xinjiang Uyghur Autonomous Region, China platransplantation@163.com
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 8451051501000580*

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Abstract:

BACKGROUND: Whether nuclear factor-kappa B/RelB, a key gene to maturation of silenced dendritic cells, can construct novel tolerogenic dendritic cells remains poorly understood?
OBJECTIVE: To investigate the effects of RelB shRNA lentivirus infection on immunological response of mouse bone marrow derived bone marrow dendritic cells.
METHODS: Mouse bone marrow derived dendritic cells were cultured with recombinant mouse granulocyte-macrophage colony-stimulating factor (rmGM-CSF) and recombinant mouse interleukin-4 (rmIL-4). RelB shRNA lentivirus was transfected into mouse bone marrow derived bone marrow dendritic cells and then divided into four groups for observation: immature dendritic cells, mature dendritic cells stimulated by lipopolysaccharide, RelB-silenced dendritic cells, and RelB-silenced dendritic cells stimulated by lipopolysaccharide.
RESULTS AND CONCLUSION: After 6 days of in vitro culture, in the group of mature dendritic cells stimulated by lipopolysaccharide, a large number of slender branch-like processes on the cell surface; in the other three groups, cell morphology was similar, exhibiting a round and shrunk appearance, and MHC-II molecule, CD86 and CD40 expression levels were similar, but they were lower compared with the group of mature dendritic cells stimulated by lipopolysaccharide. There was no significant difference in ability to stimulate T cell stimulation among the latter three groups, but the ability in these three groups was significantly lower than that in the group of mature dendritic cells stimulated by lipopolysaccharide (P < 0.01). RelB-silenced dendritic cells showed poor ability to secrete Th1 cytokineγ- interferon and IL-2 and strong ability to secrete Th2 cytokine IL-10 and IL-4 (P < 0.01). The proportion of Th1/Th2 cytokines was similar between the group of RelB-silenced dendritic cells and group of immature dendritic cells. These findings suggest that after RelB shRNA lentivirus infection, bone marrow-derived dendritic cells exhibit similar cell morphology, surface molecule expression and immunological function to immature dendritic cells and cannot be stimulated by lipopolysaccharide to develop into mature cells.

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