Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (53): 9991-9995.doi: 10.3969/j.issn.1673-8225.2011.53.027

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Effect of sensitized dendritic cells with kinectin-maltose-binding protein on T cell differentiation activity

Zhao Fei-lan, Chao Nai-xia , Li Ri-lun, Zeng Qing-tang, Huang Tian-ming, Mo Fa-rong, Xiao Fei, Luo Guo-rong   

  1. Department of Histology and Embryology, Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-03-25 Revised:2011-06-11 Online:2011-12-31 Published:2011-12-31
  • Contact: Luo Guo-rong, Doctor, Professor, Doctoral supervisor, Department of Histology and Embryology, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China gwongluo@yahoo.com
  • About author:Zhao Fei-lan☆, Doctor, Associate professor, Department of Histology and Embryology, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China zhaofeilan@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 81060168/H1602*; the Natural Science Foundation of Guangxi Zhuang Autonomous Region (Major Program), No. 2010GXNSFD013048*; a grant from Guangxi College of Traditional Chinese Medicine, No. P2008009*

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Abstract:

BACKGROUND: Kinectin, as a dendritic cell tumor seed, can be used for clinical immunotherapy of hepatocellular carcinoma.
OBJECTIVE: To evaluate the effect of kinectin-maltose-binding protein (MBP)-sensitized dendritic cells (DC) on autologous T cell differentiation activity.
METHODS: The proliferation of CD4+, CD8+ T cell subset stimulated by DC incubated with kinectin-MBP was detected by immunohistochemistry. Simultaneously, kinectin-MBP, non-sensitized DC, and commonly cultured T cells groups were set for comparison.
RESULTS AND CONCLUSION: The number of CD8+ T cells was increased significantly in the kinectin-MBP-DC-T cell group than in the kinectin-MBP, non-sensitized DC, and commonly cultured T cells groups (P < 0.05). There was no significant difference in the number of CD+ T cells and the ratio of CD4 to CD8 among the groups (P > 0.05). The results showed that kinectin-MBP-sensitized DC can effectively stimulate the proliferation of autologous CD8+ T cells and kinectin-MBP as a hepatocellular carcinoma-related antigen can exert obvious anti-tumor function via the sensitized DC.

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