Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6745-6748.doi: 10.3969/j.issn.1673-8225.2011.36.022

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Effects of cardiotrophin-1 on glutamate induced apoptosis of neural stem cells

Shen Dong-lin1, Shu Xiao-mei2, Li Zhen-hong2, Chen Xue-mei2, Du Shu-zhen2   

  1. 1Department of Pediatrics, Affiliated Hospital of Xuzhou Medical College, Xuzhou  221002, Jiangsu Province, China
    2Department of Pediatrics, the First Affiliated Hospital, Zunyi Medical College, Zunyi  563003, Guizhou Province, China
  • Received:2011-03-23 Revised:2011-06-23 Online:2011-09-03 Published:2011-09-03
  • Contact: Shu Xiao-mei, Doctor, Chief physician, Department of Pediatrics, the First Affiliated Hospital, Zunyi Medical College, Zunyi 563003, Guizhou Province, China shuxiaomei1993@sina.com
  • About author:Shen Dong-lin★, Master, Attending physician, Department of Pediatrics, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China sdlin99@sina.com
  • Supported by:

    Guizhou Governor Fund for Talents, No. 2005-86*; the Doctoral Start-up Fund of Zunyi Medical College, No. 2004-010*

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Abstract:

BACKGROUND: Cardiotrophin-1 (CT-1) appertains to interleukin-6 cytokine family, which is found in recent years to have an important protective role in the central nervous system.
OBJECTIVE: To observe the protective effects of CT-1 on the apoptosis of neural stem cells (NSCs) induced by glutamate.
METHODS: Hippocampal neural stem cells isolated from newborn Wistar rats were passaged and divided into 3 groups: glutamate, CT-1 (transfected with Adv CT-1) and control groups. NSCs survivals were estimated by MTT colorimetric method, and the apoptosis of NSCs was detected by means of TdT-Mediated dUTP nick end labeling (TUNEL).
RESULTS AND CONCLUSION: Glutamate treatment alone induced cell death that was associated with obvious morphological changes, while these changes were inhibited by transfection of CT-1 into NSCs. At 12, 24, 48 and 72 hours after treatment with glutamate, the absorbance values of MTT in the lutamate group were significantly lower than those in the CT-1 group, the apoptosis rates of NSCs in the glutamate group were significantly higher than those in the CT-1 group. At the same time, the absorbance values of MTT in the control group were significantly higher than those in the glutamate group and CT-1 group, the apoptosis rates of NSCs were significant lower than those in the glutamate group and CT-1 group. These indicated that CT-1 has a protective effect on NSCs injured by glutamate, can inhibit NSCs apoptosis and improve NSCs survival.

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