Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6749-6752.doi: 10.3969/j.issn.1673-8225.2011.36.023

Previous Articles     Next Articles

Endothelial progenitor cells pre-cultured with stromal cell derived factor 1 alpha transplantation to treat hind limb ischemia in diabetic rats

Wang Ning1, Wang Hu-guo2, Jiang Tao3, Duan Ming-jun3, Xie Zi-jing4   

  1. 1Department of No.1 Cadre Ward Medicine, 2Department of Vascular Thyroid Surgery, 3Animal Experimental Center, 4Department of Endocrinology, First Affiliated Hospital, Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Online:2011-09-03 Published:2011-09-03
  • Contact: Xie Zi-jing, Chief physician, Doctoral supervisor, Department of Endocrinology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China xiezijing@medmail.com.cn
  • About author:Wang Ning☆, Studying for doctorate, Associate chief physician, Master’s supervisor, Department of No.1 Cadre Ward Medicine, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China

PDF

356

Abstract:

BACKGROUND: Stromal cell derived factor 1 alpha (SDF-1α) has been proved to promote endothelial progenitor cells (EPCs) homing.
OBJECTIVE: To explore the effects of SDF-1α pre-cultured EPCs from autologous bone marrow on hind limb ischemia in diabetic rat models.
METHODS: Thirty male Wistar rats (successful model preparation in 25 rats) were randomly divided into 3 groups: normal saline control group (A group), EPCs transplantation in alloxan-induced diabetes rats (B group), EPCs cultured by SDF-1α transplantation in alloxan-induced diabetes rat (C group).
RESULTS AND CONCLUSION: MTT results showed SDF-1α could remarkably improve the proliferative ability of EPCs (P < 0.01). Angiography showed that angiogenesis index was significantly higher in the C group at the 28th day (P < 0.05). EPCs pre-cultured with SDF-1α have a promoted effect to reform the hind limb ischemia condition in diabetic rats; blood-supply mainly comes from new vessels and /or virgin minute blood vessel’s compensation thickening.

CLC Number: