Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6741-6744.doi: 10.3969/j.issn.1673-8225.2011.36.021

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Effect of bone marrow stromal cells in combination with exogenous cytokines on antigen expression of cord blood mononuclear cells

Zeng Jin-long1, Zeng Lian-kun1, Wang Zhi-chao1, Zhang Hai-liang1, Mao Ping2   

  1. 1People’s Hospital of Zengcheng, Zengcheng  511300, Guangdong Province, China
    2Department of Hematology, First Guangzhou Municipal People’s Hospital Affiliated to Guangzhou Medical University, Guangzhou  510182, Guangdong Province, China
  • Received:2011-06-15 Revised:2011-08-06 Online:2011-09-03 Published:2011-09-03
  • About author:Zeng Jin-long★, Master, Physician, People’s Hospital of Zengcheng, Zengcheng 511300, Guangdong Province, China chinalong_jin@163.com
  • Supported by:

    Guangdong Provincial Science and Technology Program, No. B30202*; the Science and Technology Tackle Key Program of Guangzhou Science and Technology Bureau, No. 2002z2-E0241*

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Abstract:

BACKGROUND: In vitro hematopoietic culture system of fetal bone marrow stromal cells (BMSCs) combined with exogenous cytokines has been established. It remains to verify whether this culture system can improve the proliferation of hematopoietic cells at various developmental stages.
OBJECTIVE: To investigate the effects of the culture system containing BMSCs in combination with exogenous cytokines on umbilical cord blood (UCB) mononuclear cells (MNCs) expressing CD133 and CD34.
METHODS: MNCs separated from UCB were cultured in a serum-free system with BMSCs or exogenous cytokines or both of them. On days 0, 6, 10 and 14, total nucleated cells (TNCs) were counted, CD133+, CD34+, and CD133+CD34+cells were quantitated by FACS, and hematopoietic progenitor cells were assessed by semisolid culture assay. 
RESULTS AND CONCLUSION: The number of TNCs was remarkably more in BMSCs and cytokine group compared with the other two groups. The expression of CD133+ and CD133+CD34+ cells were higher in BMSCs and cytokine group than the other two groups at days 6 and 10. The ratio of CD133+, CD34+ and CD133+CD34+ cells was significantly more in the system containing BMSCs. BMSCs in combination with cytokines can expand UCB-MNCs and CD133+, CD34+, CD133+CD34+ cells effectively. BMSCs exert an important role in remaining primitive characteristics of hematooietic stem/progenitor stem cells.

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