Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6718-6721.doi: 10.3969/j.issn.1673-8225.2011.36.016

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Isolation and aipogenic differentiation of gastric cancer stem cells

Kuang Tao1, Wang Lei2, Song Wen3, Meng Dong-mei4   

  1. 1Health Care Department, 3Department Radiology, Affiliated Hospital of Qingdao University Medical School, Qingdao  266003, Shandong Province, China; 2Department of Hematology, Qingdao Municipal Hospital, Qingdao  266071, Shandong Province, China; 4Qingdao Key Laboratory of Common Diseases-Gout Laboratory, Qingdao  266003, Shandong Province, China
  • Received:2011-04-29 Revised:2011-07-29 Online:2011-09-03 Published:2011-09-03
  • Contact: Meng Dong-mei, Doctor, Attending physician, Qingdao Key Laboratory of Common Diseases-Gout Laboratory, Qingdao 266003, Shandong Province, China mengdongmeiqd@gmail.com
  • About author:Kuang Tao, Associate chief physician, Health Care Department, Affiliated Hospital of Qingdao University Medical School, Qingdao 266003, Shandong Province, China kuangtk@126.com

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Abstract:

BACKGROUND: Whether cancer stem cells are present in all tumors is still controversial.
OBJECTIVE: To isolate and identify cancer stem cells from human gastric cancer cells, and to explore the mechanism of gastric cancer.
METHODS: Gastric cancer cells were primarily cultured and CD44 and CD29 positive expression rates were detected by flow cytometry to sort positive cells for adipogenic induction.
RESULTS AND CONCLUSION: CD44 and CD29 positive percentages in gastric cancer cells were 5.67% and 5.53%, respectively, indicating that CD44 and CD29 positive cells had an adipogenic capacity. It is proved that there are tumor stem cells with differentiation capacity in gastric cancer cells.

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