Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6722-6726.doi: 10.3969/j.issn.1673-8225.2011.36.017

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Effect of transforming growth factor beta 1 on differentiation of bone marrow mesenchymal stem cells into nucleus pulposus-like cells

Hu He1,2, Han Cheng-long1, Jiang Chao1, Shen Hong-tao1, Liu Yang1, Yan Feng1, Yu Chang-shui1, Yong Mei3   

  1. 1Department of Orthopedics, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
    2School of Medicine, Inner Mongolia University, Tongliao  028000, Inner Mongolia Autonomous Region, China
    3Inner Mongolia High School, Tongliao  028000, Inner Mongolia Autonomous Region China
  • Received:2011-03-02 Revised:2011-05-23 Online:2011-09-03 Published:2011-09-03
  • Contact: Han Cheng-long, Doctor, Associate chief physicians, Master’s supervisor, Department of Orthopedics, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China hanchenglong1973@126.com
  • About author:Hu He★, Studying for master’s degree, Department of Orthopedics, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China; School of Medicine, Inner Mongolia University, Tongliao 028000, Inner Mongolia Autonomous Region, China huhe800@163.com
  • Supported by:

    the Youth Science Foundation of Heilongjiang Province, No. QC2008C32*; Heilongjiang Postdoctoral Startup Funding & Youth Funding of the First Affiliated Hospital of Harbin Medical University, No. 2007097*

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Abstract:

BACKGROUND: Transforming growth factor beta 1 (TGF-β1) is a first selected growth factor in differentiation of bone marrow mesenchymal stem cells (BMSCs) into nucleus pulposus (NP)-like cells. Suitable concentration can induce proliferation and differentiation of BMSCs.
OBJECTIVE: To observe the effects of different-concentration TGF-β1 on differentiation of BMSCs into NP-like cells, and to optimize culture conditions.
METHODS: BMSCs were separated and purified from adult rat femur bone marrow in vitro. The third-generated BMSCs were taken and induced with different concentrations of TGF-β1-contained in HG-DMEM culture medium in the experimental group, while in control group HG-DMEM medium containing 10% fetal bovine serum was used in general state. Immunohistochemical method was used to detect collagen type Ⅱ, and RT-PCR to detect the mRNA expression of collagen type Ⅱ and proteoglycan.
RESULTS AND CONCLUSION: The proteoglycans and collagen type Ⅱ of BMSCs was significantly higher than other experimental and control groups when TGF-β1 concentration was 10 μg/L (P < 0.01). The expression of proteoglycans at 14 days were higher than that at 3, 7, 21 days in the experimental groups (P < 0.01). The expression of proteoglycans and collagen type Ⅱ in the control group was negative. TGF-β1 can increase the number of differentiated BMSCs into NP-like cells at concentration of 10 μg/L, and make BMSCs play a greater treatment efficiency.

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