Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6714-6717.doi: 10.3969/j.issn.1673-8225.2011.36.015

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Observation of morphology and distribution of CD44+/CD166+ colorectal cancer stem cells by tissue microarray

Luo Yan1,2,3, Liu Bin1,2, An Ning4, Dong Liang1, Qian Zhen1, Zhang Yong-dong3   

  1. 1Key Laboratory of Stem Cell and Gene Medicine, Department of Pathology, Lanzhou General Hospital of Lanzhou Military Command of Chinese PLA, Lanzhou  730050, Gansu Province, China
    2Department of Pathology, Basic Medical College, Lanzhou University, Lanzhou  730030, Gansu Province, China
    3Gansu Academy of Medical Science, Lanzhou 730050, Gansu Province, China
    4Tumor Hospital of Gansu Province, Lanzhou  730050, Gansu Province, China
  • Received:2011-03-02 Revised:2011-03-31 Online:2011-09-03 Published:2011-09-03
  • Contact: Liu Bin, Chief physician, Professor, Master’s supervisor, Key Laboratory of Stem Cell and Gene Medicine, Department of Pathology, Lanzhou General Hospital of Lanzhou Military Command of Chinese PLA, Lanzhou 730050, Gansu Province, China; Department of Pathology, Basic Medical College, Lanzhou University, Lanzhou 730030, Gansu Province, China liumb@189.cn
  • About author:Luo Yan★, Studying for master’s degree, Key Laboratory of Stem Cell and Gene Medicine, Department of Pathology, Lanzhou General Hospital of Lanzhou Military Command of Chinese PLA, Lanzhou 730050, Gansu Province, China; Department of Pathology, Basic Medical College, Lanzhou University, Lanzhou 730030, Gansu Province, China; Gansu Academy of Medical Science, Lanzhou 730050, Gansu Province, China Luoy1982@yeah.net
  • Supported by:

    the Natural Science Foundation of Gansu Province, No. 096RJZA096*

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Abstract:

BACKGROUND: More and more research employs CD44 as a specific marker of colorectal cancer stem cells.
OBJECTIVE: To investigate the quantity, location and distribution of colorectal cancer stem cells (Co-CSCs) CD44+/CD166+ in primary colorectal carcinoma.
METHODS: A totally of 61 cases of human colorectal carcinoma and 10 cases of normal mucosa, 18 cases of adenoma were collected and made into three tissue microarrays each containing of 42 dots respectively.
RESULTS AND CONCLUSION: The results of double-label immunohistochemical staining demonstrated there was no CD44+/CD166+ cell in normal intestine mucosa, a very small amount of CD44+/CD166+ cells in adenoma, also double-positive cells could be seen in colorectal carcinoma, the number of double-positive cells was rare and the cells were scattered or distributed focally along the basement of gland basal side. The cells with scarcely cytoplasm were square, and its nucleus was oval or high cylindrical, deep stained and homogeneous. The quantity of double-positive cells was negatively correlated with the differentiation of colorectal carcinoma. The more depth was infiltrated, the more quantity of double-positive cells was observed. To observe the number, location, distribution and morphology of Co-CSCs can contribute to the diagnosis and prognostic evaluation of colorectal carcinoma.

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