Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 5983-5986.doi: 10.3969/j.issn.1673-8225.2011.32.021

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Wound healing related trophic factors derived from human umbilical cord mesenchymal stem cells

Zhang Mei-rong, Sun Ya-ru, Wang Li, Zhang Xue-feng, Hu Jian-xia, Zhou Qian, Gao Hong, Wang Yan-gang   

  1. Stem Cell Research Center, Affiliated Hospital of Medical College, Qingdao University, Qingdao  266003, Shandong Province, China
  • Received:2011-02-14 Revised:2011-04-11 Online:2011-08-06 Published:2011-08-06
  • Contact: Sun Ya-ru, Master, Stem Cell Research Center, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China thomassun2008@yahoo.com.cn
  • About author:Zhang Mei-rong★, Master, Stem Cell Research Center, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China zhangmeirong0431@yahoo.com.cn

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Abstract:

BACKGROUND: Few reports have quantitatively determined the levels of wound healing related factors in the conditioned media of human umbilical cord mesenchymal stem cells (HUMSCs).
OBJECTIVE: To measure the secretion levels of interleukin-6 (IL-6), IL-8, transforming growth factor 1 (TGF-1), monocyte chemoattractant protein 1 (MCP-1), vascular endothelial growth factor (VEGF), GM-CSF and TIMP-1 in HUMSCs derived conditioned media of 10 different individuals.
METHODS: For culturing HUMSCs, CCS1107 was used as fetal bovine serum supplement to the culture medium. 1.1x104/cm2 P2 cells were seeded in 100 mm diameter dishes, and after 96 hours’ culture, the supernatant was collected and applied to ELISA measurement.
RESULTS AND CONCLUSION: No significant secretion difference among 10 different individuals and between genders was observed. Of total factors being measured, the levels of MCP-1 and TIMP-1 were the highest. These results indicated that HUMSCs derived conditioned media contains numerous kinds of wound healing related factors, which may clinically provide a novel therapeutic method for treating various wound healing related impairments.

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