Abstract:

BACKGROUND: Mesenchymal stem cells from Wharton’s Jelly of the human umbilical cord present with low immunogenicity and better proliferative capacity than bone marrow mesenchymal stem cells, which attract widespread attention.
OBJECTIVE: To isolate and culture mesenchymal stem cells from Wharton’s Jelly of the human umbilical cord and to study their bionomics.
METHODS: Mesenchymal stem cells were isolated and cultured from Wharton’s Jelly of the human umbilical cord by tissue cultivation. The growth curve was determined by MTT. Immunophenotype was identified by flow cytometry after dissociation.
RESULTS AND CONCLUSION: Under inversed microscope, stem cells crawled from the human umbilical cord on the second day. About seven days later, fusiform cells appeared. The shape of cells was uniform in passage 3. There was no significant change on the shape of cells in passage 7 and passage 10. The cells were still to remain fusiform.The result of MTT showed that double time of UC-MSCs in passage 3 was 3 d to 4 d. There was no significant change in the shape of passages 7 and 10 cells. UC-MSCs expressed CD44, CD29, CD105. Cells were negative for the lineage markers of CD34, CD45, CD14.