Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 5975-5978.doi: 10.3969/j.issn.1673-8225.2011.32.019

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Detection of pluripotent transcription factors in fetal dermis-derived mesenchymal stem cells

Yang Shao-guang, Xing Wen, Tian Kun, Liu Meng, Lu Shi-hong, Zhao Qin-jun, Ren Hong-ying, Pang Ai-ming, Chi Ying, Ma Feng-xia, Han Zhong-chao   

  1. State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin  300020, China
  • Received:2011-02-01 Revised:2011-05-13 Online:2011-08-06 Published:2011-08-06
  • Contact: Han Zhong-chao, Doctor, Professor, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China tihzchan@public.tpt.tj.cn
  • About author:Yan Shao-guang, Associate chief technician, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China Yangsg666@163.com Xing Wen, Master, Assistant researcher, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China xw2318@yahoo.com.cn Yang Shao-guang and Xing Wen contributed equally to this paper.
  • Supported by:

    the National 973 Program of China, No. 2011CB964800*; the National Natural Science Foundation of China, No. 30900557*; the Applied Basic and Cutting-edge Technology Research Plan of Tianjin City, No.09JCYBJC11000*

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Abstract:

BACKGROUND: The induced pluripotent stem (iPS) cells have been reprogrammed from human dermal fibroblasts and mesenchymal stem cells (MSCs). Various combinations of transcription factors are used by different investigators.
OBJECTIVE: To detect the pluripotent transcription factors expressed in fetal dermis-derived MSCs. 
METHODS: Using previous methodology in isolation and cultivation of MSCs, fetal dermis-derived MSCs were obtained from 5-month-old fetus, inducing abortion by water bag.
RESULTS AND CONCLUSION: Transcription factors of Oct4 and C-myc were expressed highly and Sox2 was expressed moderately. These results suggest that fetal dermis-derived MSCs are better somatic cells for iPS cells.

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