Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 5971-5974.doi: 10.3969/j.issn.1673-8225.2011.32.018

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Bone morphogenetic protein 9 induces skeletal muscle-derived stem cells differentiating into osteoblasts

Li Xiang, Lin Chun-yang, Qiu Bei-ming, Chen Liang, Deng Zhong-liang   

  1. Department of Orthopedics, Second Affiliated Hospital of Chongqing Medical University, Chongqing  400010, China
  • Received:2011-02-19 Revised:2011-03-28 Online:2011-08-06 Published:2011-08-06
  • Contact: Deng Zhong-liang, Doctor, Professor, Chief physician, Department of Orthopedics, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China Deng7586@gmail.com Correspondence to: Chen Liang, Doctor, Lecturer, Attending physician, Department of Orthopedics, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China Beneal.chen@gmail.com
  • About author:Li Xiang★, Studying for master’s degree, Department of Orthopedics, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China lixiang1200@gmail.com
  • Supported by:

    the Fund of Chongqing Science and Technology Committee, No. 2008BB5078*; the National Natural Science Foundation of China, No. 30772211*, 31000434*

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Abstract:

BACKGROUND: Previous studies found that some bone morphogenetic proteins can induce mesenchymal stem cells to differentiate into osteoblasts.
OBJECTIVE: To assess the capacity of bone morphogenetic protein 9 to induce the differentiation of skeletal muscle-derived stem cells into osteoblasts.
METHODS: Rabbit skeletal muscle-derived stem cells were obtained by the two-step method of collagenase-Ⅰ and trypsin, and transfected by bone morphogenetic protein 9 through recombinant adenoviruses. Then, the effect of bone morphogenetic protein 9 on the differentiation of skeletal muscle-derived stem cells into osteoblasts was observed by testing the alkaline phosphatase quantitation and calcium deposition.
RESULTS AND CONCLUSION: Muscle-derived stem cells were successfully isolated from the rabbit and over 80% of muscle-derived stem cells showed Sca-1 positive immunoreactivity. Bone morphogenetic protein 9 induced alkaline phosphatase activities in skeletal muscle-derived stem cells and promoted calcium deposition of the cells. From the above, bone morphogenetic protein 9 has the capability to induce the differentiation of skeletal muscle-derived stem cells into osteoblasts．

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