Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 5967-5970.doi: 10.3969/j.issn.1673-8225.2011.32.017

Previous Articles     Next Articles

Effects of bone morphogenetic proteins 2 on neural stem cells of 14-day-old fetal rat telencephalon differentiating into cholinergic neurons

Liang Jun1, Li Ming-qiu2, Zhao Fu-sheng1, Dong Jian-jiang1, Li Yue-zhen1   

  1. 1Department of Histology and Embryology, 2Department of Anatomy, Mudanjiang Medical College, Mudanjiang  157011, Heilongjiang Province, China
  • Received:2011-02-08 Revised:2011-03-10 Online:2011-08-06 Published:2011-08-06
  • About author:Liang Jun☆, Studying for doctorate, Associate professor, Department of Histology and Embryology, Mudanjiang Medical College, Mudanjiang 157011, Heilongjiang Province, China mdjzplj@126.com
  • Supported by:

    the Sci-Technique Project of Mudanjiang, No. G2010s0020*

PDF

409

Abstract:

BACKGROUND: Bone morphogenetic protein-2 (BMP2) may be an extracellular regulatory factor involved in cholinergic differentiation of neuronal precursor cells.
OBJECTIVE: To explore the effects of BMP2 on neural stem cells of 14-day-old fetus rat telencephalons induced into cholinergic neurons.
METHODS: Fetus telencephalons of 14-day-old SD rats were isolated, and tissues were divisively treated with collagenase typeⅠcontained EDTA following trituration. The cells were raised in polylysine culture plates with serum-free medium. Primary culture medium was changed half after 24 hours, and 10 μg/L BMP2 was added.
RESULTS AND CONCLUSION: The adherent monoculture neural stem cells could been obtained using collagenase. The Nestin positve cells were obtained, and the purity was over 99%. After induced by BMP2, the ChAT positve cells were obtained, and the purity was over 97%. BMP2 can induce neural stem cells of 14-day-old fetus rat telencephalons into cholinergic neurons.

CLC Number: