Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 5932-5935.doi: 10.3969/j.issn.1673-8225.2011.32.010

Previous Articles     Next Articles

In vitro differentiation capacity of adipose-derived stromal stem cells and bone marrow stromal stem cells

Zhou Jin, Tian Guo-ping, Wang Jing-e, Xu Bing, Li Li, Zhu Feng, Han Jian, Hu Feng-jie   

  1. Department of Neurology, First People’s Hospital of Shenyang, Shenyang  110041, Liaoning Province, China
  • Received:2011-03-13 Revised:2011-06-28 Online:2011-08-06 Published:2011-08-06
  • About author:Zhou Jin☆, Doctor, Chief physician, Department of Neurology, First People’s Hospital of Shenyang, Shenyang 110041, Liaoning Province, China zhoujinhaoyun@yahoo.com.cn
  • Supported by:

    the Fund of Shenyang Science and Technology Committee in 2009, No. 1091161-0-00*

PDF

354

Abstract:

BACKGROUND: Adipose-derived stromal stem cells (ADSCs) have many biological properties similar to bone marrow stromal stem cells (BMSCs).
OBJECTIVE: To compare the directed differentiation of ADSCs and BMSCs co-cultured with damaged PC12 cells.
METHODS: Stromal stem cells were isolated from adipose tissue and bone marrow. The fifth passage cells were selected. Two kinds of cells were co-cultured, respectively, with or without normal or damaged PC12 cells supernatant.
RESULTS AND CONCLUSION: CD44 and CD29 highly expressed in ADSCs and BMSCs, while CD45 and CD56 were only detected in BMSCs, but rare in ADSCs. After single culture, two kinds of cells highly expressed Nanog, Oct4, Sox2, but not neuron-specific enolase (NSE). The levels of Nanog, Oct4, Sox2 in the cells co-cultured with damaged PC12 cells were significantly lowered, while NSE positive cells were increased in ADSCs. These indicate that damaged PC12 cells may have a stronger effect on induce and differentiation of ADSCs. 

CLC Number: