Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 5928-5931.doi: 10.3969/j.issn.1673-8225.2011.32.009

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Comparison of bone marrow-derived mesenchymal stem cells from aborted fetus and normal adult subjects

Cheng Yue-ai, Liu Rui-feng, Zhang Yong-cui, Zhang Kai-ming   

  1. Department of Dermatology, Taiyuan Central Hospital, Taiyuan  030009, Shanxi Province, China
  • Received:2011-02-28 Revised:2011-04-26 Online:2011-08-06 Published:2011-08-06
  • Contact: Zhang Kai-ming, Doctor, Chief physician, Department of Dermatology, Taiyuan Central Hospital, Taiyuan 030009, Shanxi Province, China zhangkaiming@sina.com
  • About author:Cheng Yue-ai, Nurse in charge, Department of Dermatology, Taiyuan Central Hospital, Taiyuan 030009, Shanxi Province, China 1053881306@qq.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30972657*

Abstract:

BACKGROUND: Bone marrow-derived mesenchymal stem cells are pluripotent cells and can be involved in immune response. Fetal and adult bone marrow is both important sources for mesenchymal stem cells.
OBJECTIVE: To reveal the differences of bone marrow derived mesenchymal stem cells from aborted fetus and normal adult subjects by comparing the biological characters (including morphology, immunophenotype, proliferative activity and cytokines secretion).
METHODS: Bone marrow mononuclear cells of aborted fetus and normal adult subjects were separated by density gradient centrifugation. Bone marrow derived mesenchymal stem cells were cultured with the adherent method. Cells at passage 3 and their culture supernatants were collected. Flow cytometry analysis was used to examine the immunophenotype. The proliferation activity of cells was measured by MTT colorimetric assay. ELISA was used to measure the concentrations of cytokines secreted from bone marrow derived mesenchymal stem cells in the two groups. 
RESULTS AND CONCLUSION: Cells from both groups have the same morphology, immunophenotype and the levels of cytokines secretion. But in comparison with normal adult subjects, bone marrow derived mesenchymal stem cells from aborted fetus showed higher proliferative activity.  Bone marrow derived mesenchymal stem cells from aborted fetus and normal adult subjects have the similar biological characteristics except the proliferative activity. Aborted fetus can provide bone marrow for research of stem cells.

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