Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (45): 8501-8505.doi: 10.3969/j.issn.2095-4344.2012.45.026

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Influence of various volume fractions of platelet-rich plasma on the proliferation of rat hair follicle stem cells

Peng Yu, Wang Yu-jie, Li Jia, Muratrixat   

  1. Department of Urology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
  • Received:2012-04-09 Revised:2012-07-12 Online:2012-11-04 Published:2012-11-04
  • Contact: Muratrixat, Studying for doctorate, Associate chief physician, Department of Urology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
  • About author:Peng Yu★, Studying for master’s degree, Department of Urology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China 267305117@qq.com

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Abstract:

BACKGROUND: Recent studies have found that the platelet-rich plasma has an important role in promoting bone regeneration, soft tissue repair and proliferation and differentiation of the seed cells in tissue engineering.
OBJECTIVE: To observe the effect of different methods and different volume fractions of platelet-rich plasma on the proliferation of rat hair follicle stem cells.
METHODS: Combination of microdissection and two-step enzyme digestion with differential adhesion methods were used to obtain the third generation of rat hair follicle stem cells with high purity and good growth state. The second centrifugation method and the third centrifugation method were used to obtain the rat platelet-rich plasma, and then diluted into the K-SFM medium with the volume fraction of 1.0%, 2.0% and 4.0%, while the platelet-rich plasma containing different volume fractions of medium was considered as the experimental group and the platelet-rich plasma without medium was used as a control.
RESULTS AND CONCLUSION: Highly purified hair follicle stem cells could be obtained by microdissection, two-step enzyme digestion and differential adhesion methods. There was no significant difference between experimental group and control group at 2 days before culture (P > 0.05); and then hair follicle stem cells in the experimental group showed higher proliferation activity after the platelet-rich plasma intervention for 2 days. After cultured for 4 days, the hair follicle stem cells grew into the growth logarithmic phase; and after 5 days, the cells reached to 70%-80% confluence, and then went into the platform after cultured for 6-7 days. The cell proliferation in the high dose platelet-rich plasma group was better than that in the low dose platelet-rich plasma group. Under the condition of the same volume fraction, the effect of the platelet-rich plasma obtained by third centrifugation method on the proliferation of hair follicle stem cells was significantly greater than that obtained by the second centrifugation method. The platelet-rich plasma prepared by the third centrifugation method with the volume fraction of 4.0% has the most significant effect on the proliferation of hair follicle stem cells.

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