Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (45): 8398-8405.doi: 10.3969/j.issn.2095-4344.2012.45.008

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Superparamagnetic iron oxide nanoparticles label human bone marrow and umbilical cord mesenchymal stem cells

Ma Yan1, Zhang De-qing2, Chen Le2, Wang Jian2, Zhang Xue3, Hou Yan4, Bi Xiao-juan1, Yang Rong4, Hu An-hua5   

  1. 1Laboratory of Stem Cell Research, 3Flow Cytometry Laboratory, Clinical Medicine Research Institute of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; 2Imaging Center, 4Department of Obstetrics, 5Department of Blood Transfusion, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Received:2012-07-17 Revised:2012-08-07 Online:2012-11-04 Published:2012-11-04
  • Contact: Hu An-hua, Associate chief technician, Department of Blood Transfusion, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China Huanhua0811@126.com
  • About author:Ma Yan, Associate chief technician, Laboratory of Stem Cell Research, Clinical Medicine Research Institute of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China mayan1121@126.com

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Abstract:

BACKGROUND: Nowadays, it is becoming more and more important to optimize safety of human derived cells, label cells efficiently and track cells after cells transplantation both in basic research and clinic application.
OBJECTIVE: To compare the cell viability, labeling efficiency and imaging effect of the T2* weight image (WI) magnetic resonance (MR) between the human bone marrow and umbilical cord derived mesenchymal stem cells labeled with the superparamaganetic iron oxide nanoparticles, as well as to optimize their treatment efficiency.
METHODS: The third generation of human bone marrow and umbilical cord derived mesenchymal stem cells were cultured, and labeled with 5-30 mg/L Feridex Ⅳ and protamine sulfate.
RESULTS AND CONCLUSION: The viability of human bone marrow mesenchymal stromal cells was similar with human umbilical cord derived mesenchymal stem cells (P > 0.05). There was no significant difference of labeling rate between the bone marrow msenchymal stem cells labeled with 5-30 mg/L Feridex Ⅳ (P > 0.05); while there was significant difference of labeling rate between the umbilical cord derived mesenchymal stem cells labeled with 5 mg/L Feridex Ⅳ and 20 and 30 mg/L Feridex Ⅳ (P < 0.05); the positive labeling rate of umbilical cord derived mesenchymal stem cells was lower than that of bone marrow msenchymal stem cells after labeled with 10 mg/L Feridex Ⅳ (P < 0.05). When two sources of cells were labeled with Feridex Ⅳ more than 2 mg/L, the iron oxide particles were found in the cell suspension and could not be removed by elution and filtration. The signal intensity from 3.0T MR GRE T2*WI scan was decreased with the increasing of Feridex Ⅳ concentration in both cell types. It is safe and effective to label the two tissue-derived mesenchymal stem cells with 10 mg/L Feridex Ⅳ-protamine sulfate complex, and can be observed with T2*WI MR.

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