Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (15): 2716-2720.doi: 10.3969/j.issn.1673-8225.2011.15.014

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Establishment of a new rat model of portal vein arterialization

Wu Jun-zheng1, Jiang Yi1, Zhang Xiao-jin2, Lü Li-zhi2   

  1. 1School of Clinical Medicine, Fuzhou General Hospital of Nanjing Military Region, Fujian Medical University, Fuzhou  350025, Fujian Province, China
    2Department of Hepatobiliary Surgery, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou  350025, Fujian Province, China
  • Received:2010-10-19 Revised:2010-12-19 Online:2011-04-09 Published:2013-11-06
  • Contact: Jiang Yi, Doctor, Professor, Doctoral supervisor, School of Clinical Medicine, Fuzhou General Hospital of Nanjing Military Region, Fujian Medical University, Fuzhou 350025, Fujian Province, China jiangyi183@yahoo. com.cn
  • About author:Wu Jun-zheng★, Studying for master’s degree, School of Clinical Medicine, Fuzhou General Hospital of Nanjing Military Region, Fujian Medical University, Fuzhou 350025, Fujian Province, China dr.wujunzheng@ gmail.com
  • Supported by:

    the Scientific Research Foundation for Medical Science of Nanjing Military Region, China, No. 08MA104*

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Abstract:

BACKGROUND: At present literature reported that portal vein arterializations model of rats with suture method or stent method in the blood patency and operating simplicity are not perfect.
OBJECTIVE: To establish a stable and simple model of portal vein arterialization in rats. 
METHODS: A total of 40 cases of portal vein arterializations model in rats were established by the allogeneic vascular embolia suture method and cuff method. Left kidney was removed. Left renal artery was connected with portal vein stump by allogeneic vascular; the left renal vein was connected with superior mesenteric vein by cuff method. Another 10 rats served as sham operation group. The body weight and survival rate were observed after surgery. The rats were executed magnetic resonance imaging (MRI) after 2 weeks. Hepatic function of rats was detected at 1 month after surgery. Two months after surgery, all rats were killed to probe portal vein patency, and hepatic pathological changes were observed.
RESULTS AND CONCLUSION: One rat dead during modeling, the remaining 39 rats survived after two months. The successful rate of operation was 97.5% (39/40). At one month after surgery, there was no significant difference in body weight, albumin, alanine aminotransferase, alkaline phosphatase, and cholinesterase between model group and sham operation group (P > 0.05). Two months after surgery, the patency rate of portal vein was 94.9% (37/39) in model group. Both MRI and pathological results showed no obvious abnormalities. Based on the allogeneic vascular embolia suture method and cuff method, the rat model of portal vein arterialization have properties in simple operation, time-saving and high successful rate, which is a viable, stable, reliable and reproducible method.

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