Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (15): 2703-2706.doi: 10.3969/j.issn.1673-8225.2011.15.011

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Interventional effect of pravastatin on vascular endothelial growth factor expression in rabbits with steroid-induced avascular necrosis of the femoral head

Hu Min1, Fan Jian-nan2, Zhao Hong-bin3, Qian Chuan-yun3, Wei Wei3, Liu Yan-kun4, Zhang Yang3   

  1. 1Clinical Specialty, Medical College of Kunming University, Kunming  650214, Yunnan Province, China
    2Department of Orthopedics, Affiliated Hospital of Guiyang Medical College, Guiyang  550004, Guizhou Province, China
    3Department of Orthopedics, First Affiliated Hospital of Kunming Medical College, Kunming  650032, Yunnan Province, China
    4Kunming Science and Technology Agency, Kunming  650500, Yunnan Province, China
  • Received:2010-12-04 Revised:2011-03-06 Online:2011-04-09 Published:2013-11-06
  • Contact: Zhao Hong-bin, Doctor, Associate professor, Department of Orthopedics, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China 576794680@qq.com
  • About author:Hu Min, Associate professor, Clinical Specialty, Medical College of Kunming University, Kunming 650214, Yunnan Province, China humin999@ yahoo.cn Fan Jian-nan, Associate professor, Department of Orthopedics, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China Hu Min and Fan Jian-nan contributed equally to this paper.
  • Supported by:

    the National Natural Science Foundation of China, No. 81060361*, 30860389*; Natural Science Foundation of Yunnan Province, No. 2010ZC169*; Research Foundation of Department of Education of Yunnan Province, No.2010C212*; Research Foundation of Science and Technology of Kunming, No.10S090202*

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Abstract:

BACKGROUND: Previous research has demonstrated that pravastatin can effectively promote endogenous vascular endothelial growth factor (VEGF) mRNA in endothelial cells from human umbilical vein cultured in vitro, but the research of protein VEGF expression in the femoral heads of rabbits with steroid-induced avascular necrosis of the femoral head (SANFH) after treatment with pravastatin is rare now.
OBJECTIVE: To evaluate the endogenous protein VEGF expression in the femoral heads of SANFH rabbit models after treatment with pravastatin.
METHODS: Totally 80 New Zealand white rabbits were randomly divided into a normal control group (n=18) and experimental group (n=62). Rabbits in the experimental group were prepared for SANFH models. At 5 weeks after model preparation, 36 rabbits in the model group were assigned into the model and pravastatin groups, with 18 animals in each group. Rabbits in the pravastatin group received intragastric administration with 1.2 mg/kg pravastatin once per day, and the same volume of distilled water was given to those in the model and control groups. Six rabbits in each group were killed at the 8th, 12th and 16th week respectively to evaluation of protein VEGF expression in the femoral heads by immunohistochemistry.
RESULTS AND CONCLUSION: In the normal control group, endogenous expression of VEGF mRNA was positive at each time point. In the model group, 8 weeks after modeling, there was negative VEGF mRNA expression; 12 weeks after modeling positive expression; 16 weeks after modeling weakly positive expression. In the pravastatin group, 8 weeks after modeling, VEGF mRNA showed weak positive expression; 12 weeks after modeling positive expression; 16 weeks after modeling positive expression, but lower than 12 weeks after modeling slightly weakened. Pravastatin can effectively promote endogenous VEGF mRNA in early SANFH rabbit models.

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