Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (15): 2699-2702.doi: 10.3969/j.issn.1673-8225.2011.15.010

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Establishment of rabbit intervertebral disc degeneration models using percutaneous annulus fibrosus puncture

Wan Zhong-yuan1, 2, Li Fang2, Li Jin-zhen2, 3, Ren Da-jiang2   

  1. 1Fourth Military Medical University of Chinese PLA, Xi’an  710032, Shaanxi Province, China
    2Orthopaedic Research Institute, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing  100700, China
    3Shanxi Medical University, Taiyuan  030001, Shanxi Province, China
  • Received:2011-01-13 Revised:2011-03-04 Online:2011-04-09 Published:2013-11-06
  • Contact: Li Fang, Professor, Master’s supervisor, Orthopaedic Research Institute, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing 100700, China FANGL6722@sina. com
  • About author:Wan Zhong-yuan★, Studying for master’s degree, Physician, Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China; Orthopaedic Research Institute, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing 100700, China wanzhongyuan@ yeah.net

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Abstract:

BACKGROUND: Although an advisable experimental animal model is necessary for the intervertebral disc tissue engineering, currently, there is not an accepted experimental animal models preparation method.
OBJECTIVE: To detect the feasibility of the establishment of the rabbit intervertebral disc degeneration animal model by means of C-arm-assisted percutaneous annulus fibrosus puncture.
METHODS: L2/3 and L3/4 disc of New Zealand white rabbit were selected as puncture intervention discs, the L1/2, L5/6 disc were selected as blank control, the intervention group discs were treated by percutaneous annulus fibrosus puncture with the aids of C-arm. Two rabbits were anesthetized at 2, 4, 8, and 12 weeks respectively after operation for MRI images. Then the animals were killed, the change of lumbar intervertebral discs was observed by MRI, and nucleus proteoglycan contents were measured by dimethylmethyleneblue (DMMB) dye spectrophotometry.
RESULTS AND CONCLUSION: Signal intensity and proteoglycan content of the nucleus pulposus of the intervention group showed a progressive downtrend at 4 weeks after intervention (P < 0.05), but, by contrast, signal intensity and proteoglycan content of the nucleus pulposus in the control group were gradually decreased. C-arm-assisted percutaneous annulus fibrosus puncture is a feasible method in intervertebral disc degeneration animal model preparation.

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