Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (45): 8361-8366.doi: 10.3969/j.issn.2095-4344.2012.45.001

    Next Articles

Expression of transient receptor potential vanilloid receptor 5 during osteogenic differentiation of bone marrow mesenchymal stem cells

Li Fu-chun1, Ma Xue-ling2, Qu Yan-ping3, Gu Gui-shan4   

  1. 1First Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
    2Department of Neurology, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
    3Department of Out-patient, Heilongjiang Provincial Hospital, Harbin 150001, Heilongjiang Province, China
    4First Department of Orthopedics, the First Clinical Medical College of Jilin University, Changchun 130021, Jilin Province, China
  • Received:2012-08-09 Revised:2012-09-03 Online:2012-11-04 Published:2012-11-04
  • Contact: Gu Gui-shan, Doctoral supervisor, Professor, First Department of Orthopedics, the First Clinical Medical College of Jilin University, Changchun 130021, Jilin Province, China guguishan@sina.com
  • About author:Li Fu-chun☆, Doctor, Attending physician, First Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China lfcmxl@163.com

PDF

554

Abstract:

BACKGROUND: At present, the calcium ion channel research is confined in the electrophysiological properties demonstrated by transient receptor potential vanilla receptor 5 in the kidney, small intestine and smooth oocytes.
OBJECTIVE: To explore the expression and significance of new calcium ion channel transient receptor potential vanilla receptor 5 in rat bone marrow mesenchymal stem cells derived osteoblasts.
METHODS: The rat bone marrow mesenchymal stem cells were separated, extracted and cultivated, then the rat bone marrow mesenchymal stem cells were induced to differentiate into osteoblasts, the expression of transient receptor potential vanilla receptor 5 in osteoblasts derived from rat bone marrow mesenchymal stem cells was detected by immunohistochemistry.
RESULTS AND CONCLUSION: The in vitro cultured rat bone marrow mesenchymal stem cells were successfully induced to differentiate into osteoblasts. Alkaline phosphatase staining and alizarin red staining results showed that the purple-black and red mineralized nodules were visible in the cytoplasm of the osteoblasts derived from rat bone marrow mesenchymal stem cells. Immunohistochemical staining results showed the strongly positive expression of transient receptor potential vanilla receptor 5 in bone marrow mesenchymal stem cells derived osteoblasts. Results confirm that transient receptor potential vanilla receptor 5 calcium ion channel exists in rat bone marrow mesenchymal stem cells derived osteoblasts, and the transient receptor potential vanilla receptor 5 calcium ion channel may be the candidate channel which can regulate the osteoblasts proliferation and differentiation.

CLC Number: