Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (37): 6682-6688.doi: 10.3969/j.issn.2095-4344.2013.37.023

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Expression of minichromosome maintenance 3 from the peripheral blood of fluorosis patients and the liver and renal function

Zhang Ya-lou1, Zhao Yang2, Tang Li3, Wu Qi-qing1, Bai Sheng-bin1, Zhong Jin-jie1   

  1. 1Department of Histology and Embryology, Basic Medical Institute, Xinjiang Medical University, Urumqi  830011, Xinjiang Uygur Autonomous Region, China; 2Department of Burns and Plastic Surgery, 3Infectious Disease Centre, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830011, Xinjiang Uygur Autonomous Region, China
  • Received:2013-03-07 Revised:2013-04-28 Online:2013-09-10 Published:2013-09-10
  • Contact: Zhong Jin-jie, Professor, Department of Histology and Embryology, Basic Medical Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China zhongjinjie@gmail.com
  • About author:Zhang Ya-lou☆, M.D., Associate professor, Department of Histology and Embryology, Basic Medical Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China zhangyalou12@yahoo.com.cn Zhao Yang☆, Studying for doctorate, Associate chief physician, Department of Burns and Plastic Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China gorgezy@163.com Zhang Ya-lou and Zhao Yang contributed equally to this study.
  • Supported by:

    National Natural Science Foundation of China, No. 30860249*, 81102084*

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Abstract:

BACKGROUND: Previous studies have shown that minichromosome maintenance 3 is related with fluorosis, but the expression of minichromosome maintenance 3 in fluorosis patients is not clear yet.
OBJECTIVE: To analyze the mRNA expression level of minichromosome maintenance 3 in peripheral blood from patients exposed to fluoride and normal controls.
METHODS: Eleven patients with mild fluorosis by drinking water (exposure group) and 11 cases of control (non-exposure group) were selected for research. SYBRGreen1 real-time quantitative PCR was used to determine the mRNA expression of minichromosome maintenance 3 in peripheral blood mononuclear cells, and the liver and renal function indicators were detected.
RESULTS AND CONCLUSION: mRNA expressions of minichromosome maintenance 3 in the exposure group and non-exposure group were (0.573 60±0.102 59) and (0.550 0±0.171 81), respectively, and there was no significant difference between two groups (P > 0.05). There were no significant differences in the liver and renal function indicators between two groups. The results indicate that mild fluorosis has no significant effect on mRNA expression of minichromosome maintenance 3 in the peripheral blood mononuclear cells. More indicators are needed to compressively analyze the effect of fluoride on the liver and renal functions.

Key words: fluorine, genes, endemic diseases, reverse transcriptase polymerase chain reaction

CLC Number: