Occlusion of dentinal tubules using tricalcium silicate

doi:10.3969/j.issn.2095-4344.2013.38.006

Abstract

Abstract:

BACKGROUND: A number of in vitro experiments have confirmed that the tricalcium silicate not only can be closely integrated with the dentin through self-curing process, but also can induce dentin remineralization in the physiological environment, thereby effectively blocking the dentinal tubules.
OBJECTIVE: To further verify the effects of tricalcium silicate solution on the occlusion of dentinal tubules.
METHODS: Thirty-six dentinal discs were made of free first premolars from orthodontic patients, and divided into three pretreatment groups randomly. The teeth were soaked in pretreatment solution for 2 minutes, namely 0.29 mol/L ethylene diamine tetraacetie acid, 6% citric acid, and rinsed ultrasonically with deionized water 20 minutes, respectively. Every above-mentioned group was randomly assigned into experimental group (tricalcium silicate), control group (sodium fluoride) and blank group, and corresponding materials in each group were used to coat the outer dentinal tubules (2 minutes/time). Then, the dentinal discs were saved in artificial saliva in a 37 ℃ thermostat. After 14 days, the morphology of the dentinal tubules before and after treatment was observed using scanning electron microscope. Diameter and area of open dentinal tubules were calculated.
RESULTS AND CONCLUSION: After pretreatment, the dentinal tubules were at open state; except for the blank control group to maintain the original state, acid etching and ethylene diamine tetraacetie acid pretreatment solutions had a stronger capacity of demineralization, which led to the dentinal tubules open. After the dentinal tubules were treated with sodium fluoride and tricalcium silicate, there were varying degrees of sediments, and open dentinal tubule area and average diameter in the sodium fluoride and tricalcium silicate groups were lower than those in the control group (P < 0.05). The dentinal tubule treated with tricalcium silicate was almost entirely closed homogeneously, and occasionally, a single open dentinal tubule was seen. Open dentinal tubule area and average diameter in the tricalcium silicate group were significantly lower than those in the sodium fluoride group (P < 0.05). The findings verify that dentin occlusion using tricalcium silicate is superior to that using sodium fluoride; and dentin tubule pretreatment with acid etching or ethylene diamine tetraacetie acid is beneficial to desensitization effects.

Key words: biocompatible materials, dentin, dentin sensitivity, pit and fissure sealants

CLC Number:

R318

Yang Qing-ling, Chen Si-jie, Wan Yin, Geng Cong, Rong Guang-ying. Occlusion of dentinal tubules using tricalcium silicate[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.38.006.

Figures/Tables 4

References

[1] 中华口腔医学会牙本质敏感专家组.牙本质敏感的诊断和防治指南[J].中华口腔医学杂志,2009,44(3):132-134.
[2] PaesLeme AF,dosSantos JC,Giannini M,et al.Occlusion of dentin tubles by desensitizing agents.Am J Dent.2004;17(5): 3682372.
[3] Al-Sabbagh M,Andreana S,Ciancio SG.Dentinal hypersensitivity: review of aetiology,differential diagnosis, prevalence,and mechanism.J IntAcad Periodontol. 2004;6(1): 8212.
[4] 樊明文.牙体牙髓病学[M].北京:人民卫生出版社,2003:134-137.
[5] 孙卫斌,袁留平,俞未一,等.复方高良姜糊剂牙本质小管堵塞作用的实验研究(Ⅱ)[J].牙体牙髓牙周病学杂志,1996,6(3):139-141.
[6] Hirvonen TJ,Närhi MVO,Hakumaki MOK.The excitability of dog pulp nerves in relation to the condition of dentine surface. J Endod.1984;10:294-298.
[7] Uchida A,Ishikawa Y,Ishida H.Transmission electron microscopic characterization of hypersensitive human radicular dentin. J Dent Res.1990;69:1293.
[8] 郑麟藩,张震康,俞光岩.实用口腔科学[M].2版.北京:人民卫生出版社,2000:34-36.
[9] American Dental Association.Survey of consumer attitudes and behaviors regarding dental issues.Chicago:American Dental Association,1997.
[10] Dederich DN,Bushick RD.Laser in dentistry separating science from hype. J Am Dent Assoc.2004;135(2):204-212.
[11] 刘颖婷, 高辉.氟保护漆治疗牙本质敏感症临床疗效观察[J].中国误诊学杂志,2007,7(2):256.
[12] 周红文,陈亚明,祁惠兰,等.氟离子导入治疗牙本质敏感症的临床研究[J].口腔医学,2007,27(10):523-524.
[13] Itota T,Torii Y,Nakabo S,et al.Effect of fluoride-re-leasing adhesive system on decalcified dentin.J Oral Rehabil. 2003; 30(2):178-183.
[14] Suge T,Kawasaki A,Ishikawa K,et al.Ammonium hexafluorosilicate elicits calcium phosphate precipitation and shows continuous dentin tubule occlusion Dent Mater.2008; 24(2):192-198.
[15] 王晓虹,常江,孙宏晨.硅酸三钙阻塞牙本质小管的实验研究[J].口腔医学研究,2007,23(2): 176-178.
[16] Pashley DH.Dentin permeability,dentin sensitivity,and treatment through tubule occlusion.J Endod.1986;12(10): 465-467.
[17] 熊伯刚,熊世江.牙本质过敏症治疗的研究进展[J].国际口腔医学杂志,2008,35(3):292-294.
[18] 梁登忠,关淑妮.牙本质过敏症治疗进展[J].广东牙病防治,2001, 9(4): 313-314.
[19] 周红文,陈亚明,祁惠兰,等.氟离子导入治疗牙本质敏感症的临床研究[J].口腔医学,2007,27(10):523-524.
[20] 李玉晶,李建英,杨玉琴.氟化氨银抑制牙本质龋机制的探讨[J].北京口腔医学,1997,42 (4):18-20.
[21] Sauro S,Gandolfi MG,Prati C,et al.Oxalate-containing phytocomplexes as dentine desensitisers:An in vitrostudy. Arch Oral Biol. 2006;51(8):655-664.
[22] Dragolich WE,Pashley DH,Brennan WA,et al.An in vitro study ofdentinal tubule occlusion by ferric oxalate. J Periodontol. 1993;64:1045-1051.
[23] Pereira JC,Angela D.Effect of desensitizing agents on the hydraulic conductance ofhuman dentin subjected to different surface pre-treatments-an invitro study. Dent Mater.2005; 21:129-138.
[24] Imai Y,Akimoto T.A new method of treatment for dentin hypersensitivity by precipitation of calcium phosphate in situ. Dent Mater J.1990;9:167-172.
[25] Ishikawa K,Suge T,Yoshiyama M,et al.Occlusion of dentinal tubules with calcium phosphate using acidic calcium phosphate solution followed byneutralization.J Dent Res. 1994;73(6):1197-1204.
[26] Gillam DG,Tang JY,Mordan NJ,et al.The effects of a novel bioglass? dentifrice on dentine sensitivity: a scanning electron microscopy investigation.J Ora Reha.2002;29:305-313.
[27] Zhao WY,Wang JY,Zhai WY,et al.The self-setting properties and in vitro bioactivity of tricalcium silicate. Biomaterials. 2005;26(31):6113-6121.
[28] Siriphannon P,Kameshima Y,Yasumori A,et al.Formation of hydroxyapatite onCaSiO3 powders in simulated body fluid.J Euro Ceram Soc.2002;22:511-520.
[29] Kokubo T,Kushitani C,Ohtsuki C,et al.Process of formation of bone-like apatite layer on silica gel.J Mater Sci Mater Med. 1993; 4:127-131.
[30] Cho SB,Miyaji F,Kokubo T,et al.Apatite-forming ability of silicate ion dissolved from silica gels. J Biomed Mater Res. 1996;32(3):375-381.
[31] Torabinejad M,Hong CU,Pitt Ford TR,et al.Cytotoxicity of four root-end filling materials.J Endod.1995;21(8):489-492.
[32] 张向宇,孙志滢.深龋综合治疗法的疗效[J].牙体牙髓牙周病学杂志,2003,13(11):621.