Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (6): 1070-1074.doi: 10.3969/j.issn.1673-8225.2011.06.026

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Side population cells in human lymphoma Raji cell line: Possibility of lymphoma stem cells

Xie Jian1, Li Xiao-jin2, Jin Cong-guo3, Yu Mei-jia2, Li Hui-min1   

  1. 1Department of Hematology, First Affiliated Hospital, Kunming Medical University, Kunming  650021, Yunnan Province, China
    2Department of Hematology, Fourth Affiliated Hospital, Kunming Medical University, Kunming  650031, Yunnan Province, China
    3Institute of Tumor, Third Affiliated Hospital, Kunming Medical University, Kunming  650106, Yunnan Province, China
  • Received:2010-07-07 Revised:2010-10-18 Online:2011-02-05 Published:2011-02-05
  • Contact: Li Hui-min, Professor, Chief physician, Department of Hematology, First Affiliated Hospital, Kunming Medical University, Kunming 650021, Yunnan Province, China lihuimin@medmail.com.cn
  • About author:Xie Jian★, Master, Physician, Department of Hematology, First Affiliated Hospital, Kunming Medical University, Kunming 650021, Yunnan Province, China ls_xiejian@163.com
  • Supported by:

    the Natural Science Foundation of Yunnan Province, No. 2006C0076M*

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Abstract:

BACKGROUND: Previous studies have demonstrated that cancer stem cells enriched SP cells in the acute leukemia and multiple myeloma. Moreover, immunophenotype and biological characteristics have significant heterogeneity compared with main population cells. However, there is few study concerning lymphoma stem cells. 
OBJECTIVE: To examine whether the human lymphoma cell line-Raji contains SP cells, to investigate the difference in differentiation antigen, P-glycoprotein expression and cell cycle between SP cells and non-SP cells, and to explore the existing possibility of lymphoma stem cells.
METHODS: After Hoechst-33342 staining, Raji cell line SP cells were detected and sorted using flow cytometry with ultraviolet. Inhibitory effects of various mass concentrations of verapamil on SP cells were observed and an optimal inhibitory concentration was selected. After sorting, P-glycoprotein, differentiation antigen CD34, CD20, CD19, CD5 and cell cycle were determined and compared between SP and non-SP cells.
RESULTS AND CONCLUSION: SP cells accounted for about 2% of viable cells in Raji, and could be blocked when verapamil was 50 mg/L. SP expressed 18.2% CD20+ cells compared with non-SP (93.6% CD20+), 78.0% CD5+cells compared with non-SP (22.2% CD5+). No significant difference in P-glycoprotein expression was detected between the two populations, but the P-glycoprotein (+) cells in SP displayed more intensive fluorescence compared with non-SP. These indicated that there are significant differences in differentiation degree and multidrug resistance between SP cells and non-SP cells in Raji cell line. SP cells are heterogeneous subset. We supposed that Raji cell line might be organized similarly as cancer stem cell hierarchy, and it is possible that lymphoma stem cells are enriched in SP cells.

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