Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (6): 967-971.doi: 10.3969/j.issn.1673-8225.2011.06.005

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Promoting effects of laminin on osteogenetic differentiation of umbilical cord mesenchymal stem cells and focal adhesion kinase expression

Zha La Ga-hu1, Xu Zhong-wei1, Lan Xiao-xia2, Chen Xiao3, Chen Xiao-yi1   

  1. 1Department of Cell Biology, Medical College of Chinese People’s Armed Police Forces, Tianjin  300162, China
    2Teaching Room of Epidemiology, Department of Preclinical Medicine, Medical College of Chinese People’s Armed Police Forces, Tianjin  300162, China
    3Department of Gynaecology and Obstetrics, Affiliated Hospital, Medical College of Chinese People’s Armed Police Forces, Tianjin  300162, China
  • Received:2010-09-04 Revised:2010-10-10 Online:2011-02-05 Published:2011-02-05
  • Contact: Chen Xiao-yi, Master, Professor, Department of Cell Biology, Medical College of Chinese People’s Armed Police Forces, Tianjin 300162, China chenxiaoyi6111@163.com
  • About author:Zha La Ga-hu★, Master, Lecturer, Department of Cell Biology, Medical College of Chinese People’s Armed Police Forces, Tianjin 300162, China zhalaga2003@sina.com
  • Supported by:

    the Scientific Research Program of Chinese People’s Armed Police Forces, No. WY200806*

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Abstract:

BACKGROUND: Recent studies on umbilical cord mesenchymal stem cells (UCMSCs) are focused on the isolation, purification and induction and differentiation of UCMSCs, and there are a few reports about the studies of the effects of extracellular matrix components and correlations to signaling pathway.
OBJECTIVE: To study the effects of laminin on the osteogenesis differentiation of UCMSCs and expression of focal adhesion kinase (FAK).
METHODS: The logarithmic cells were divided in to four groups. In laminin induction group, laminin concentrations were at 50, 25, 5, 1 mg/L. In osteogenic induction group, osteogenic solution was used. In co-induction group, laminin and osteogenic solution were used. In control group, no Laminin or osteogenic solution. Observation of cell morphology, Alizarin red staining analysis of calcium salt deposits, detection of alkaline phosphatase (ALP) activity, histochemical and western blotting analysis of ERK1/2, P-ERK and FAK expression were performed after 4 weeks of culture.
RESULTS AND CONCLUSION: ①The cells of induction groups were formed to short column and linked together with short neurites, and nucleus appeared roundness under inverted microscopes. ② Alizarin red staining analysis indicated that the cells were positive with red color. Co-induction group appeared more calcium salt deposits than other groups. Calcium salt deposits were higher in 21-day cells than other time points cells (P< 0.05). ③ ALP activity analysis indicated that laminin induction group had higher ALP activity than control group, and 7-day induction cells had statistical significant differences (P < 0.05). There were no differences among the cells treated with different laminin concentrations (P > 0.05). ④ Histochemical analysis indicated that the expressions of ERK1/2, P-ERK and FAK were improved in laminin induction group compared with control group (P < 0.05). ⑤ Western blotting analysis indicated that there were ERK1/2, P-ERK and FAK proteins in induction and co-induction groups, but no above-mentioned expression in laminin induction and control groups. Results indicated that laminin promoted the differentiation of UCMSCs into osteoblasts and upregulated ERK1/2, P-ERK and FAK protein expression.

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