Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (1): 46-50.doi: 10.3969/j.issn.1673-8225.2011. 01.010

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Construction of rat models of myocardial ischemia/reperfusion injury following bone marrow mesenchymal stem cell pretransplantation for 1 week  

Xu Feng, Wang Ai-ling, Chen Feng, Zhou Mei-ling   

  1. Department of Cardiology, First Affiliated Hospital, Anhui Medical University, Hefei  230022, Anhui Province, China
  • Received:2010-08-24 Revised:2010-11-01 Online:2011-01-01 Published:2011-01-01
  • Contact: Wang Ai-ling, Doctoral supervisor, Department of Cardiology, First Affiliated Hospital, Anhui Medical University, Hefei 230022, Anhui Province, China
  • About author:Xu Feng★, Studying for master’s degree, Department of Cardiology, First Affiliated Hospital, Anhui Medical University, Hefei 230022, Anhui Province, China xufeng929@sina.com

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Abstract:

BACKGROUND: Blocked blood vessels were opened following acute myocardial infarction with thrombolysis or intervention method. It is difficult to reach a satisfactory outcome due to myocardial ischemia/reperfusion injury.
OBJECTIVE: To observe the repair effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on myocardial ischemia/reperfusion injury in rats.
METHODS: BMSCs were cultured and expanded from healthy 3-week-old Sprague-Dawley rats in vitro. Recipient Sprague-Dawley rats were injected with BMSCs or serum-free DMEM. 1 week later, rat models of myocardial ischemia/reperfusion injury were established. Following myocardial ischemia for 0.5 hour and reperfusion for 2 hours, serum lactate dehydrogenase (LDH) activity, total superoxide dismutase (TSOD) vitality and malondialdehyde (MDA) levels of myocardial tissue were measured. Cell apoptotic index was examined by terminal deoxynucleotidyl transferase-mediated d-UTP nick end labeling. The expressions of Bcl-2 and Bax proteins were measured by immunohistochemical technique.
RESULTS AND CONCLUSION: BMSCs not only had a high purification, homogeneity and stability, but also had unlimited proliferation. Compared with ischemia/reperfusion group, LDH leakage was reduced, TSOD activity was increased and MDA levels were diminished in the BMSCs group (P < 0.01). Apoptotic index was significantly lower in the BMSCs group compared with ischemia/reperfusion group (P < 0.01). Bcl-2 expressions were greater in the BMSCs group compared with ischemia/reperfusion group (P < 0.05), but the Bax/Bcl-2 ratio in the BMSCs group was lower than the ischemia/reperfusion group (P < 0.05). Results suggest that BMSCs transplantation can promote cell proliferation of rats with myocardial ischemia/reperfusion injury.

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