Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (1): 23-28.

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Angiogenesis in a rat model of focal cerebral ischemia following adipose tissue-derived neural stem cell transplantation

Liu Bin1, Liu Ning1, 2, Dong Jing1, Wang Rui-min3, Zhang Jin-xia1   

  1. 1First Department of Neurology, Affiliated Hospital of North China Coal Medical College, Tangshan  063000, Hebei Province, China
    2Emergency Department, Kailuan Hospital Affiliated to North China Coal Medical College, Tangshan  063000, Hebei Province, China
    3Experimental Center, North China Coal Medical College, Tangshan 063000, Hebei Province, China
  • Received:2010-07-26 Revised:2010-08-10 Online:2011-01-01 Published:2011-01-01
  • About author:Liu Bin★, Master, Professor, Chief physician, First Department of Neurology, Affiliated Hospital of North China Coal Medical College, Tangshan 063000, Hebei Province, China liubin919tsh@sina.com
  • Supported by:

    the Natural Science Foundation of Hebei Province, No. C2008000994; the Abroad Training Foundation for Outstanding Experts of Hebei Province, No. 2007; the Scientific Research Foundation of Tangshan City, No. 08130204a-1-21

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Abstract:

BACKGROUND: Adipose tissue-derived neural stem cells transplantation may improve neurological function in rats with cerebral ischemia injury, but its mechanism remains unclear.
OBJECTIVE: To study the effects of human adipose tissue-derived neural stem cells transplantation on angiogenesis in rats after focal cerebral ischemia.
METHODS: Adipose tissue-derived stromal cells cultured in vitro were differentiated into neural stem cells. A total of 60 healthy male Sprague-Dawley rats were assigned into four groups randomly: normal control group (n=6), sham-operated group (n=6), ischemia control group (n=24) and transplantation treated group (n=24). Models of 2-hour ischemia/reperfusion of rat middle cerebral artery were established by suture-occluded method in the ischemia control group and transplantation treated group. Ischemia control group and transplantation treated group were divided randomly into 7, 14, 21 and 28 days reperfusion groups (n=6). Middle cerebral artery was not occluded in the sham-operated group. On hour 24 following model induction, human adipose tissue-derived neural stem cell suspension (2×109 /L) was given in the transplantation treated group via the caudal vein. Saline was injected into the rats in ischemia control group via the caudal vein. Immunohistochemistry was used to identify microvessel density (MVD). The blood vessel proliferation in the rat focal cerebral ischemia area was observed. 
RESULTS AND CONCLUSION: Immunohistochemistry results have exhibited that compared with the ischemia control group, the MVD was significantly greater at 7, 14, 21, 28 days after transplantation in the transplantation treated group (P < 0.05-0.01). Results indicated that neural stem cells differentiated from human adipose tissue-derived stromal cells transplantation can promote the angiogenesis in cerebral ischemia regions.

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