Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (1): 7-11.

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Effects of injured spinal cord extracts on brain-derived neurotropic factor and myelin proteolipid protein in bone marrow mesenchymal stem cells

Liu Ran1, 2, Fan Dong-yan3, Jin Peng4, Fan Hong-xue1, Wang Ping4   

  1. 1College of Public Health, Jilin University, Changchun  130021, Jilin Province, China
    2Changchun Blood Center, Changchun  130031, Jilin Province, China
    3Medical College of Xizang University, Lasa  850000, Xizang Autonomous Region, China
    4Department of Otolaryngology, Head and Neck Surgery, First Hospital, Jilin University, Changchun  130021, Jilin Province, China
  • Received:2010-09-20 Revised:2010-11-22 Online:2011-01-01 Published:2011-01-01
  • Contact: Wang Ping, Doctor, Associate researcher, Department of Otolaryngology, Head and Neck Surgery, First Hospital, Jilin University, Changchun 130021, Jilin Province, China wangping99@hotmail.com
  • About author:Liu Ran★, Master, Attending physician, College of Public Health, Jilin University, Changchun 130021, Jilin Province, China; Changchun Blood Center, Changchun 130031, Jilin Province, China lr770920@163.com

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Abstract:

BACKGROUND: The component of injured spinal cord extracts is complex and includes some chemical substances or cytokines. It remains unclear if these factors may influence cellular proliferation and secretion of bone marrow mesenchymal stem cells (BMSCs) or not.  
OBJECTIVE: To investigate the effect of injured spinal cord extracts on secretion of brain-derived neurotropic factor (BDNF) or myelin proteolipid protein (PLP) in BMSCs.
METHODS: BMSCs were obtained from Wistar rats. After 3 generation of cell proliferation, BMSCs were induced with normal or injured spinal cord extracts for 20 days. Neuron specific enolase (NSE) positive cells were detected by immunofluorescence staining. Levels of BDNF and PLP in BMSCs cultured supernatant were evaluated using enzyme linked immunosorbent assay (ELISA) and BDNF and PLP mRNA was determined by real time polymerase chain reaction assay.
RESULTS AND CONCLUSION: The rate of NSE positive cells and levels of BDNF and PLP that were treated with injured spinal cord extracts were greater significantly at different cultured time points than normal spinal cord extracts. These results showed that the injured spinal cord extracts could induce secretion of BDNF and PLP in BMSCs that is to the benefit of cellular differentiation into neuronal cells.

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