Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (23): 4262-4266.doi: 10.3969/j.issn.1673-8225.2010.23.019

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Colonization and differentiation of allogeneic rat bone marrow mesenchymal stem cells transplanted into the intestine with ischemia/reperfusion injury

Gao Guang-zhou1, Li Da-wei2, Li Xin3, Sun Tao3   

  1. 1Navy Clinical Medical College, Second Military Medical University of Chinese PLA, Beijing  100037, China; 2ICU, 3Department of Gastroenterology, Second Military Medical University of Chinese PLA, Beijing  100037, China
  • Online:2010-06-04 Published:2010-06-04
  • Contact: Sun Tao, Professor, Chief physician, Master’s supervisor, Department of Gastroenterology, Second Military Medical University of Chinese PLA, Beijing 100037, China
  • About author:Gao Guang-zhou, Studying for master’s degree, Physician, Navy Clinical Medical College, Second Military Medical University of Chinese PLA, Beijing 100037, China gaoguangzhou@ yahoo.com.cn
  • Supported by:

    the Military General Program during the 11th Five-Year Plan Period, No. 06MA017*

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Abstract:

BACKGROUND: The small intestine is extremely sensitive to the hypoxia and ischemia, and it would be got severe damage after ischemia/reperfusion injury. Bone marrow mesenchymal stem cells (BMSCs) are multipotent cells which might participate in the repair of damaged tissue through a variety of ways.

OBJECTIVE: To investigate colonization and therapeutic effects of allogeneic rat BMSCs transplantation in the intestine of ischemia/reperfusion injury model.

METHODS: Wistar female rats were assigned to three groups. The abdominal cavity was opened and then sutured in the sham operation group. Remaining rats were used to establish ischemia/reperfusion models. Rats in the control group were only infused with saline following intestine with ischemia/reperfusion injury. Rats in the treatment group were treated with BMSCs from Wistar male rats via caudal vein. Following treatment, jejunal tissue was harvested at 12, 24 hours, 3, 7, 14 and 28 days separately to make frozen sections. The distribution of donor cells in the receptor intestine was observed under a fluorescence microscope. Reverse transcription-polymerase chain reaction was employed to determine the sex-determining gene (SRY) in male rats. The levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the jejunal tissues were detected.

RESULTS AND CONCLUSION: No donor cell transfused in intestinal villi was detected under the fluorescence microscope. The expression ratio of the SRY gene was 50%, 66.6%, 33.3%, 16.6% at 3, 7, 14, and 28 days respectively. The levels of MDA were lower, but SOD levels were higher in jejunum tissues in the treatment group than the control group on 12, 24 hours, 3 and 7 days. Results have found that BMSCs from allogeneic rats could colonize in the injured intestine of the receptor rat with ischemia/reperfusion injury. The BMSCs transplantation can promote the recovery of intestinal injury. The beneficial effects of BMSCs were primarily mediated via paracrine actions but not by their direct differentiation into target cells.

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