Immune function monitoring for rejection and infection after renal transplantation

doi:10.3969/j.issn.2095-4344.2013.05.008

Abstract

Abstract:

BACKGROUND: Cylex ImmuKnow is the first and only U S Food and Drug Administration-cleared blood test to measure immune cell function, it can reflect the immune function of the cells directly, which is a valuable tool with good qualities of high sensitivity and specificity.
OBJECTIVE: To investigate the correlation between immune cell function and the rejection and infection after renal transplantation through monitoring of immune function intracellular adenosine triphosphatei value.
METHODS: We collected 140 renal transplant patients. The immune cell function levels were measured by ImmuKnowTM-Cylex®. The patients were divided into three groups according to clinical status of patients, namely, rejection group (n=18), infection group (n=35) and stability group (n=87). In addition, the cellular immune function data in the blood of 61 samples from healthy people were collected as control group. Then the correlation of the immune cell function and infection to rejection after renal transplantation was analyzed.
RESULTS AND CONCLUSION: The measurement of intracellular adenosine triphosphatei value showed that about 71.4% (n=25) patients in the infection group distributed in the low immune response zone, and the percentage in the infection group was significantly higher than that in the other three groups (P < 0.05). The intracellular adenosine triphosphatei value in the infection group was significantly lower than that in the other three groups. That indicated that the infection was correlated with low cellular mediated immune function, and the detection of immune cell function played an important role in monitoring the infection after renal transplantation. The immune cell function monitoring with ImmuKnowTM-Cylex® assay provides reliable and objective basis for the diagnosis and treatment of infection after renal transplantation, but it has no correlation with the rejection after renal transplantation, and that needs further investigation with a large-sample experiment.

Key words: organ transplantation, renal transplantation, immune cell function, immune, cell immune, rejection, infection, intracellular adenosine triphosphatei value, ImmuknowTM, provincial grants-supported paper

CLC Number:

R318

Wang Qing-hua, Shang Le-le, Chen Tai-chun, Wang Jin, Tang Min-ying, Wu Lin, Tan Jian-ming. Immune function monitoring for rejection and infection after renal transplantation[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(5): 811-816.

Figures/Tables 3

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