Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (19): 3539-3543.doi: 10.3969/j.issn.1673-8225.2010.19.024

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Intervention of Taohong Siwu Decoction on adipogenic differentiation of steroid-induced bone marrow mesenchymal stem cells

Li Shu-qiang1, Yu Tao1, Qi Zhen-xi2   

  1. 1Department of Orthopaedics and Traumatology, Fujian Traditional Chinese Medical College, Fuzhou  350108, Fujian Province, China;
    2Institute of Bone Disease, Fujian Traditional Chinese Medical College Academy of Integrated Traditional and Western Medicine, Fuzhou  350108, Fujian Province, China
  • Online:2010-05-07 Published:2010-05-07
  • Contact: Qi Zhen-xi, Doctor, Professor, Doctoral supervisor, Institute of Bone Disease, Fujian Traditional Chinese Medical College Academy of Integrated Traditional and Western Medicine, Fuzhou 350108, Fujian Province, China zxqi@fjtcm.edu.cn
  • About author:Li Shu-qiang, Master, Department of Orthopaedics and Traumatology, Fujian Traditional Chinese Medical College, Fuzhou 350108, Fujian Province, China lshq921@yahoo.com. cn
  • Supported by:

    the Development Fund for Integrative Medicine of Chen Keji, No. CKJ2007006*

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Abstract:

BACKGROUND: Clinical pathological and histological research has verified that the early pathological changes of steroid-induced avascular necrosis of femoral head are reduced bone marrow hematopoietic tissue and increased adipose tissue in femoral head and which may be related to adipogenic differentiation of steroid-induced bone marrow mesenchymal stem cells (BMSCs). These pathological changes are currently being used to explain the pathogenesis of steroid-induced avascular necrosis of the femoral head.
OBJECTIVE: To investigate effects of Taohong Siwu Decoction on adipogenic differentiation of steroid-induced BMSCs.
METHODS: Rat BMSCs were cultured in vitro. High-dose steroid was used to induce adipogenic differentiation of BMSCs in vitro, at the same time, Taohong Siwu Decoction containing serum was given. After 6 days of intervention, adipogenic markers triglyceride, PPARγ mRNA and aP2 mRNA expression were determined.
RESULTS AND CONCLUSION: Taohong Siwu Decoction containing serum could resist triglyceride, PPARγ mRNA and aP2 mRNA expression in steroid-induced BMSCs. These indicated that mechanism of Taohong Siwu Decoction to prevent and cure the avascular necrosis of the femoral head is not only to improve the microcirculation, but also is associated with inhibitory effects on steroid-induced adipogenic differentiation of BMSCs.

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