Platelet-rich plasma effects on grainy fat transplantation

doi:10.3969/j.issn.2095-4344.2013.31.011

Abstract

Abstract:

BACKGROUND: The higher long-term absorption rate greatly influence the widely application of fat transplantation. Platelet-rich plasma contains a high concentration of growth factors, which benefits to the tissue healing and regeneration.
OBJECTIVE: To observe the effects of platelet-rich plasma on grainy fat transplantation and to investigate the mechanisms preliminarily.
METHODS: Ten 6-week-old nude mice were prepared. The right or left dorsal subcutaneous tissues were randomly selected as the platelet-rich plasma group (0.5 mL fat granule+0.1 mL platelet-rich plasma), and the contralateral side was regarded as the control group (0.5 mL fat granule+0.1 mL phosphate-buffered saline). At 10 and 90 days after implantation, five nude mice were selected from each group, and then the mice were sacrificed to obtain the grafts in each group for general appearance observation, volume determination and histological detection. Furthermore, we isolated adipose-derived stem cells from human subcutaneous fat tissue during the in vitro experiment. Cell counting kit-8 and real-time PCR were used to evaluate the influence of platelet-rich plasma on adipose-derived stem cell proliferation and adipogenic differentiation in vitro, respectively.
RESULTS AND CONCLUSION: Comparison of the grafts obtained at 10 and 90 days after implantation, the residual volume in the platelet-rich plasma group was significantly larger than that in the control group (P < 0.05), Moreover, more normal adipocytes and capillary formation were observed in the platelet-rich plasma group (P < 0.05). For in vitro experiment, platelet-rich plasma could significantly improve adipose-derived stem cell proliferation, and the expressions of adipogenic-related genes were up-regulated in platelet-rich plasma-induced adipose-derived stem cells. All results demonstrate that platelet-rich plasma can improve the survival of fat grafts, which might be closely related to that platelet-rich plasma can promote the proliferation and adipogenic differentiation of adipose-derived stem cells and the revascularization in grafted fat tissue.

Key words: organ transplantation, cell transplantation, platelet-rich plasma, adipose-derived stem cells, angiogenesis, cell proliferation, adipogenic differentiation, National Natural Science Foundation of China

CLC Number:

R318

Li Feng, Li Kun, Li Jie, Tian Wei-dong. Platelet-rich plasma effects on grainy fat transplantation[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.31.011.

Figures/Tables 7

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