Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (28): 4554-4558.doi: 10.12307/2023.683
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Fu Dongge, He Jingzi
Received:
2022-08-22
Accepted:
2022-10-19
Online:
2023-10-08
Published:
2023-01-29
Contact:
He Jingzi, PhD, Physical Culture Institute, Yanan University, Yanan 716000, Shaanxi Province, China
About author:
Fu Dongge, PhD, Physical Culture Institute, Yanan University, Yanan 716000, Shaanxi Province, China
Supported by:
CLC Number:
Fu Dongge, He Jingzi. Key pathways and hub genes of osteoarthritis based on transcriptome data of sports injury synovial tissue[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(28): 4554-4558.
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2.1 差异表达基因的筛选 研究选择的数据集GSE55457,包括10例骨关节炎滑膜组织样本和10例健康者滑膜组织样本,所选样本转录组数据分布情况见图1A,其UMAP图见图1B。基于筛选标准adjust P < 0.05,共筛选出1 574个差异表达基因,包括1 122个上调表达基因,452个下调表达基因,火山图如图1C。数据集GSE55235,包括10例骨关节炎滑膜组织样本和10例健康者滑膜组织样本,所选样本转录组数据分布情况见图1D,其UMAP图见图1E。基于筛选标准adjust P < 0.05,共筛选出1 816个差异表达基因,包括721个上调表达基因,1 095个下调表达基因,火山图如图1F。随后,对上下调基因分别取两数据集的交集,见图2,其中上调基因200个,下调基因124个。取两数据集前100个上调基因的交集,共获得8个基因(JUN,ZFP36L2,DUSP1,MAFF,GRB10,APOLD1,VEGFA,FKBP5);取其前100个下调基因的交集,共获得9个基因(EPYC,SCRG1,GAP43,CD300A,WNT5B,CX3CR1,IGKC,TLR7,PART1)。"
2.2 差异基因的功能富集分析 将筛选得到的324个差异表达基因进行GO功能注释和KEGG富集分析,GO分析结果表明差异表达基因生物学过程主要和脂肪细胞分化、RNA剪接调控、通过剪接体调控mRNA的剪接、转移酶活性的负调控、激酶活性的负调控有关;细胞组分主要和核斑点、细胞质核糖核蛋白颗粒、核糖核蛋白颗粒、Copi涂层囊泡、细胞质应激颗粒有关,而分子功能主要与DNA结合转录因子结合、RNA聚合酶Ⅱ特异性DNA结合转录因子结合、poly(A) 结合、血小板源性生长因子受体结合、糖皮质激素受体结合有关,见图3。而KEGG主要富集在MAPK信号通路、胰岛素信号通路、破骨细胞分化、甲状旁腺激素的合成、分泌和作用等通路,见图4。"
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