Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (32): 5155-5161.doi: 10.12307/2022.879
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Li Haoliang, Li Dongfang
Received:
2021-07-22
Accepted:
2021-11-29
Online:
2022-11-18
Published:
2022-05-12
Contact:
Li Dongfang, Master, Attending physician, Department of Traumatology and Orthopaedics, Second Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000, Henan Province, China
About author:
Li Haoliang, Master, Attending physician, Department of Traumatology and Orthopaedics, Second Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000, Henan Province, China
CLC Number:
Li Haoliang, Li Dongfang. Emodin promotes fracture healing in osteoporotic fracture rats by inhibiting miR-338-3p expression[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(32): 5155-5161.
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2.1 实验动物数量分析 53只大鼠全部进入结果分析。 2.2 各组大鼠骨密度比较 卵巢切除3个月后检测大鼠右侧股骨中段及L5骨密度,与假手术组大鼠相比,卵巢切除大鼠腰椎骨密度下降(P < 0.05),说明骨质疏松模型造模成功,见图1A。 大鼠经过不同浓度大黄素和miR-338-3p mimic处理,8周后检测大鼠股骨中段及L5骨密度,与单纯骨折组相比,大黄素低、中、高剂量组大鼠股骨中段及L5骨密度升高(P < 0.05),且呈剂量依赖性;大黄素高剂量+miR-338-3p mimic组股骨中段和腰椎骨密度高于骨折组(P < 0.05),低于大黄素高剂量组(P < 0.05),见图1B、C。 "
苏木精-伊红染色显示,大黄素高、中、低剂量组中软骨性骨痂组织少于骨性骨痂组织,且骨小梁较粗、致密、数量多,大黄素高剂量组骨小梁结构更密集、排列整齐,单纯骨折组骨小梁较细、间隙大、结构较乱和疏松;大黄素高剂量+miR-338-3p mimic组骨小梁也较细,但间隙较小,结构较为疏松。 定量分析结果显示,单纯骨折组骨小梁面积低于假手术组(P < 0.05),大黄素低、中、高剂量组骨小梁面积高于单纯骨折组(P < 0.05),且呈剂量依赖性;大黄素高剂量+miR-338-3p mimic组骨小梁面积低于大黄素高剂量组P < 0.05),高于单纯骨折组(P < 0.05)。 2.4 各组大鼠骨痂组织中miR-338-3p的表达 RT-qPCR检测结果显示,单纯骨折组miR-338-3p mRNA表达水平高于假手术组(P < 0.05);与单纯骨折组相比,大黄素低、中、高剂量组miR-338-3p mRNA表达水平降低,且呈剂量依赖性(P < 0.05);大黄素高剂量+miR-338-3p mimic组miR-338-3p mRNA表达水平高于大黄素高剂量组和单纯骨折组(P < 0.05),见图3。 "
与假手术组相比,单纯骨折组血清NO水平降低(P < 0.05);与单纯骨折组相比,大黄素低、中、高剂量组血清NO水平升高(P < 0.05),且具有剂量依赖性。大黄素高剂量+miR-338-3p mimic组血清NO水平高于单纯骨折组(P < 0.05),低于大黄素高剂量组(P < 0.05)。与假手术组相比,单纯骨折组血清肿瘤坏死因子α和白细胞介素1β水平升高(P < 0.05);与单纯骨折组相比,大黄素低、中、高剂量组血清肿瘤坏死因子α和白细胞介素1β水平降低(P < 0.05),且呈剂量依赖性(P < 0.05)。大黄素高剂量+miR-338-3p mimic组血清肿瘤坏死因子α和白细胞介素1β水平高于大黄素高剂量组(P < 0.05),低于单纯骨折组(P < 0.05)。 2.6 各组大鼠骨痂组织中成骨相关蛋白表达 见图5。 "
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