Echinops latifolius, a Mongolian medicine, for treating cartilage injury in a rabbit model of knee osteoarthritis

doi:10.12307/2022.529

Abstract

Abstract: BACKGROUND: Osteoarthritis is clinically common, which is the most common cause of chronic disability in middle-aged and elderly people. Echinops latifolius, as a Mongolian medicine, has positive therapeutic effect in bone diseases. However, its effect and mechanism on osteoarthritis remain to be investigated.
OBJECTIVE: To investigate the effect of Echinops latifoliuss on knee osteoarthritis and on the expression of type II collagen and matrix metalloproteinase 13.
METHODS: Thirty healthy Chinese white rabbits were selected and randomly divided into a control group (n=10) and a knee osteoarthritis group (n=20). Rabbits in the knee osteoarthritis group were fixed with cast plaster for 8 weeks to prepare knee osteoarthritis models. After successful modeling, rabbits in the knee osteoarthritis group were subdivided into an Echinops latifolius group and a model control group. In the Echinops latifolius group, 3.25 g/L Echinops latifolius was administered at a dose of 20 mL/kg by gavage. The control and model control groups were intragastrically administered normal saline at a dose of 20 mL/kg. Rabbits in each group were given continuous gastric gavage for 90 days, and then killed under anesthesia. Knee joint cartilage was taken and sliced. Hematoxylin-eosin staining was use to observe the histological changes of cartilage. RT-PCR was used to detect the mRNA expression of type II collagen and matrix metalloproteinase 13, and immunohistochemical staining was used to detect the protein expression of type II collagen and matrix metalloproteinase 13.
RESULTS AND CONCLUSION: Echinops latifolius could improve osteoarthritis-induced cartilage damage of and promote the proliferation of chondrocytes and matrix. In addition, Echinops latifolius could promote the mRNA and protein expression of type II collagen in osteoarthritis, and inhibit the high expression of matrix metalloproteinase 13 at mRNA and protein levels caused by osteoarthritis. To conclude, Echinops latifolius can promote the expression of type II collagen and inhibit the expression of matrix metalloproteinase 13, thereby playing a positive role in the prevention and treatment of osteoarthritis.

Key words: osteoarthritis, Mongolian medicine, Echinops latifolius, type II collagen, matrix metalloproteinase 13, ethnic medicine

CLC Number:

Kong Lingyue, Hu Yongcheng, Han Changxu. Echinops latifolius, a Mongolian medicine, for treating cartilage injury in a rabbit model of knee osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(17): 2649-2653.

Figures/Tables 3

References

[1] IANNONE F, LAPADULA G. The pathophysiology of osteoarthritis. Aging Clin Exp Res. 2003;15(5):364-372.
[2] MOLLOY MG, MOLLOY CB. Contact sport and osteoarthritis. Br J Sports Med. 2011;45(4):275-277.
[3] CORYELL PR, DIEKMAN BO, LOESER RF. Mechanisms and therapeutic implications of cellular senescence in osteoarthritis. Nat Rev Rheumatol. 2021;17(1):47-57.
[4] CHARLIER E, DEROYER C, CIREGIA F, et al. Chondrocyte dedifferentiation and osteoarthritis (OA). Biochem Pharmacol. 2019;65:49-65.
[5] MESSINA OD, VIDAL WILMAN M, VIDAL NEIRA LF. Nutrition, osteoarthritis and cartilage metabolism. Aging Clin Exp Res. 2019;31(6): 807-813.
[6] ATTUR MG, PATEL IR, PATEL RN, et al. Autocrine production of IL-1 beta by human osteoarthritis-affected cartilage and differential regulation of endogenous nitric oxide, IL-6, prostaglandin E2, and IL-8. Proc Assoc Am Physicians.1998; 110(1):65-72.
[7] ZHENG L, ZHANG Z, SHENG P, et al. The role of metabolism in chondrocyte dysfunction and the progression of osteoarthritis. Ageing Res Rev. 2021;66:101249.
[8] HENROTIN Y, ADDISON S, KRAUS V, et al. Type II collagen markers in osteoarthritis: what do they indicate? Current Opinion in Rheumatology. 2007;19(5):444-450.
[9] VINCENTI M, BRINCKERHOFF C. Transcriptional regulation of collagenase (MMP- 1, MMP-13) genes in arthritis: integration of complex signaling pathways for the recruitment of gene-specific transcription factors. Arthritis Res. 2002;4(3):157-164.
[10] SHIOMI T, LEMAÎTRE V, D’ARMIENTO J, et al. Matrix metalloproteinases, a disintegrin and metalloproteinases, and a disintegrin and metalloproteinases with thrombospondin motifs in non-neoplastic diseases. Pathol Int. 2010;60(7):477-496.
[11] GAO F, ZHANG S. Salicin inhibits AGE-induced degradation of type II collagen and aggrecan in human SW1353 chondrocytes: therapeutic potential in osteoarthritis. Artif Cells Nanomed Biotechnol. 2019; 47(1):1043-1049.
[12] 王宏伟,杨婷,董玉. 蓝刺头的研究进展[J].疾病监测与控制杂志, 2011,5(12):734-735.
[13] 巴音额古乐,金鸿宾. 蒙药蓝刺头对骨折愈合过程中BMP-2及VEGF表达影响[J].中国民族医药杂志,2017,6(6):53-55.
[14] 杨斌,萨茹丽,张月梅,等.蓝刺头多糖体外抗氧化及抗菌活性的研究[J].华北农学报,2016,31(4):233-238.
[15] 姜梦如,刘丹丹,朱浩,等.蓝刺头及蓝刺头复方水提物对实验性Ⅱ型糖尿病大鼠血糖及血脂的影响[J]. 中国兽医学报,2017, 37(5):15-17.
[16] WANG J, DONG X, MA F,et al. Metabolomics profiling reveals Echinops latifolius Tausch improves the trabecular micro-architecture of ovariectomized rats mainly via intervening amino acids and glycerophospholipids metabolism. J Ethnopharmacol. 2020;260: 113018.
[17] 刘岩,鞠亚波,常虹.蒙药蓝刺头对去卵巢模鼠骨钙素影响的实验研究[J].内蒙古医科大学学报,2014,36(s1):7-11.
[18] LIU Y, WANG X, CHANG H, et al. Mongolian Medicine echinops prevented postmenopausal osteoporosis and induced ER/AKT/ERK pathway in BMSCs. Biosci Trends. 2018;12(3):275-281.
[19] 邬波,马旭,柳椰,等.膝关节骨关节炎患者软骨炎症因子表达与病变程度的相关性[J]. 中国组织工程研究,2020,24(2):78-83.
[20] BLOM AB, LENT P, LIBREGTS S, et al. Crucial role of macrophages in matrix metalloproteinase-mediated cartilage destruction during experimental osteoarthritis: involvement of matrix metalloproteinase 3. Arthritis Rheumatol. 2010;56(1):147-157.
[21] JI B, MA Y, WANG H, et al. Activation of the P38/CREB/MMP13 axis is associated with osteoarthritis. Drug Des Devel Ther. 2019;13:2195-2204.
[22] ZHANG W, ZHANG C, LUO C, et al. Design, cyclization, and optimization of MMP13-TIMP1 interaction-derived self-inhibitory peptides against chondrocyte senescence in osteoarthritis. Int J Biol Macromol. 2019; 121:921-929.
[23] ZHANG J, ZHANG W, SHI J, et al. Dlx2 overexpression enhanced accumulation of type II collagen and aggrecan by inhibiting MMP13 expression in mice chondrocytes. Biochem Biophys Res Commun. 2018; S0006291X18311215.
[24] SACITHARAN PK. Ageing and Osteoarthritis. Subcell Biochem. 2019;91: 123-159.
[25] DICKSON BM, ROELOFS AJ, ROCHFORD JJ, et al. The burden of metabolic syndrome on osteoarthritic joints. Arthritis Res Ther. 2019;21(1):289.
[26] VERZIJL N, DEGROOT J, ZAKEN CB, et al. Crosslinking by advanced glycation end products increases the stiffness of the collagen network in human articular cartilage: a possible mechanism through which age is a risk factor for osteoarthritis. Arthritis Rheuma. 2002;46(1):114-123.
[27] QU H, LI J, WU L, et al. Trichostatin A increases the TIMP-1/MMP ratio to protect against osteoarthritis in an animal model of thedisease. Mol Med Rep. 2016;14:2423-2430.
[28] HAO HQ, ZHANG JF, HE QQ, et al. Cartilage oligomeric matrix protein, C-terminal cross-linking telopeptide of type II collagen, and matrix metalloproteinase-3 as biomarkers for knee and hip osteoarthritis (OA) diagnosis: a systematic review and meta-analysis. Osteoarthritis Cartilage. 2019;27(5):726-736.
[29] DAI M, SUI B, XUE Y, et al. Cartilage repair in degenerative osteoarthritis mediated by squid type II collagen via immunomodulating activation of M2 macrophages, inhibiting apoptosis and hypertrophy of chondrocytes. Biomaterials. 2018;180:91-103.
[30] SUN K, LUO J, JING X, et al. Hyperoside ameliorates the progression of osteoarthritis: An in vitro and in vivo study. Phytomedicine. 2021;80: 153387.
[31] 廖建钊, 章晓云, 张璇. 骨性关节炎发生发展中的分子信号通路[J]. 中国组织工程研究,2020,24(21):3394-3400.