Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (11): 1720-1727.doi: 10.12307/2022.357
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Liang Xuezhen1, Xie Guoxin2, Li Jiacheng1, Wen Mingtao2, Xu Bo1, Li Gang1, 3
Received:
2020-11-23
Revised:
2020-11-28
Accepted:
2021-03-27
Online:
2022-04-18
Published:
2021-12-11
Contact:
Li Gang, MD, Professor, Chief physician, The First Clinical Medical School, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong Province, China; Department of Orthopedic Microsurgery, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China
About author:
Liang Xuezhen, MD, Lecturer, Attending physician, The First Clinical Medical School, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong Province, China
Supported by:
CLC Number:
Liang Xuezhen, Xie Guoxin, Li Jiacheng, Wen Mingtao, Xu Bo, Li Gang. Identification and analysis of potential key genes in osteonecrosis of the femoral head based on miRNA-mRNA regulatory network[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(11): 1720-1727.
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2.1 差异表达的miRNA GSE89587数据集中股骨颈骨折术后发生股骨头坏死和股骨颈骨折术后愈合患者的血清样本相比,获取差异表达 miRNA有23个,包括10个表达上调 (hsa-miR-4325,hsa-miR-3654,hsa-miR-3194-3p,hsa-miR-384,hsa-miR-335-5p,hsa-miRPlus-C1066,hsa-miR-32-5p,hsa-let-7d-5p,hsa-miR-374c-5p,hsa-miR-452-5p)和13个表达下调(hsa-miR-4680-5p,hsa-miR-4711-3p,hsa-miR-3127-3p,hsa-miR-3157-3p,hsa-miR-3920,hsa-miR-1207-3p,hsa-miR-4725-3p,hsa-miR-3124-3p,hsa-miR-606,hsv1-miR-H1-3p,hsa-miR-3191-5p,hsa-miR-3675-5p,hsa-miR-4688),各miRNA的火山图和聚类图,见图1。"
2.5 网络靶基因功能分析结果 通过R软件clusterProfiler包对miRNA-mRNA调控网络中的靶基因进行GO功能和KEGG通路富集分析,得到218个GO富集条目,其中包括150个BP条目、33个CC条目和35个MF条目。BP主要富集在病毒过程的正调控、病毒基因组复制的正调控、RNA稳定、液泡组织、细胞色素沉着、调节mRNA稳定性、负调控mRNA分解过程、RNA稳定性的调节、RNA分解过程的负调控及调节mRNA分解过程等方面;CC主要富集在运输囊泡膜、细胞质应激颗粒、催化步骤2剪接体、跨高尔基网络膜、鞘氨醇涂层囊泡膜、核染色质、跨高尔基网络运输囊泡膜及间隔细胞骨架等;MF主要富集在单链RNA结合、poly(A)结合、转录因子结合、poly(U) RNA结合及嘌呤束结合等,富集前5个BP、CC和MF条目见图6A。为显示更小的高维数据子集,使用R软件GOplot包绘制弦图(Chord)和簇图(Cluster)以展示最显著富集的5个GO结果,见图6B,6C。KEGG通路富集分析得到4条信号通路,主要为转化生长因子β信号通路、糖胺聚糖生物合成-硫酸角聚糖、溶酶体及自噬等,见图7,表4。"
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