Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (2): 225-231.doi: 10.12307/2022.037
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Lai Han1, Wang Jiao1, Dong Miaomiao1, Luo Meng1, Wang Wenhao1, Zhou Guoping1, 2
Received:
2020-09-17
Revised:
2020-09-17
Accepted:
2020-10-30
Online:
2022-01-18
Published:
2021-10-27
Contact:
Zhou Guoping, Chief physician, Doctoral supervisor, Hospital of Integrated Traditional Chinese Medicine and Western Medicine, Southern Medical University, Guangzhou 510315, Guangdong Province, China; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, Guangdong province, China
About author:
Lai Han, Master candidate, Hospital of Integrated Tranditonal Chinese Medicine and Western Medicine, Southern Medical University, Guangzhou 510315, Guangdong Province, China
Supported by:
CLC Number:
Lai Han, Wang Jiao, Dong Miaomiao, Luo Meng, Wang Wenhao, Zhou Guoping. Electroacupuncture intervenes with changes of mitogen-activated protein kinase pathway in a rat model of cerebral ischemia/reperfusion due to middle cerebral artery occlusion[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(2): 225-231.
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2.4 各组大鼠海马CA1区p-ERK、p-p38MAPK、p-JNK累积吸光度值的比较 2.4.1 p-ERK及p-p38MAPK双染结果的比较 假手术组中各时间亚组p-ERK、p38MAPK均有少量表达,组间表达水平比较无差异(P > 0.05)。模型组、电针组与假手术组比较差异有显著性意义(P < 0.05)。模型组p-ERK、p-p38MAPK两种指标在脑缺血再灌注2 h时表达均有升高趋势,p-ERK在6 h迅速到达峰值后逐渐回落;p-p38MAPK在3 d时达高峰(P < 0.05)。与模型组比较,脑缺血再灌注2 h时,电针组即出现p-ERK表达的上调(P < 0.05),1,3 d时间亚组上调明显,差异显著(P < 0.05);电针组p-p38MAPK表达水平与模型组相比较均有所下降,在6 h、3 d时间亚组较明显(P < 0.05)。见表3及图4,5。"
2.4.3 p-JNK及p-ERK双染结果的比较 假手术组各时间亚组p-ERK、p-JNK均有少量表达,各时间亚组间表达水平无统计学差异(P > 0.05)。模型组、电针组与假手术组比较差异有统计学意义(P < 0.05)。模型组p-ERK在2 h出现上调,在6 h迅速到达峰值后逐渐回落;p-JNK约在1 d时表达量达高峰后呈下降趋势。与模型组比较,脑缺血再灌注2 h时电针组即出现p-ERK表达的上调(P < 0.05)以及p-JNK表达的下调(P < 0.05),其中1 d、3 d时间亚组p-ERK上调明显(P < 0.05),而p-JNK的下调1 d、3 d亚组更显著(P < 0.05)。见表5,图10, 11。"
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