Effects of different induced polarization methods on the proliferation, apoptosis and phagocytosis of rat bone marrow-derived macrophages

doi:10.12307/2021.016

Abstract

Abstract: BACKGROUND: Classically activated and alternative activated macrophages have different phenotypes and functions, and there are few studies to explore the effect of different induction methods on the biological functions of bone marrow-derived macrophages.
OBJECTIVE: To explore the effect of different induced polarization ways on biological behaviors of bone marrow-derived macrophages from rats.
METHODS: Rat bone marrow-derived macrophages were isolated and cultured in vitro, and identified by morphological observation, Wright Giemsa staining and flow cytometry. After being induced and activated by interferon-γ, lipopolysaccharide and interleukin-4 for 24 hours, the morphology of bone marrow-derived macrophages was observed by inverted microscope. The expression levels of cell surface markers Nos2 and Arg1 were detected by real time-PCR. CCK8 assay was performed to determine the proliferation ability of bone marrow-derived macrophages. Flow cytometry was performed to detect the apoptosis, and neutral red staining was performed to identify the phagocytic function of bone marrow-derived macrophages.
RESULTS AND CONCLUSION: Bone marrow-derived macrophages that stimulated by interferon-γ and lipopolysaccharide were highly expressing the cell surface marker Nos2 of M1, and apoptosis of bone marrow-derived macrophages can be slightly promoted as well as the phagocytic function of bone marrow-derived macrophages is reduced; while bone marrow-derived macrophages stimulated by interleukin-4 are highly expressing the cell surface marker Arg1 of M2. Cell proliferation and phagocytosis of bone marrow-derived macrophages are promoted, and apoptosis of bone marrow-derived macrophages is inhibited. The results confirm that the biological behavior of bone marrow-derived macrophages can be affected by different methods of induced polarization.

Key words: bone marrow, macrophages, type 1 macrophages, type 2 macrophages, polarization, γ-interferon, lipopolysaccharide, interleukin-4

CLC Number:

Li Li, Zhuo Jin, Zheng Ling, Zhou Ling, Wang Qisong, Luo Lan, Luo Kai. Effects of different induced polarization methods on the proliferation, apoptosis and phagocytosis of rat bone marrow-derived macrophages[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(25): 4032-4037.

Figures/Tables 7

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